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多环芳烃荧蒽诱导拟南芥氧化胁迫

刘泓1;叶媛蓓1;崔波2;郑荔敏1;黄炎和1;王宗华2   

  1. 1福建农林大学资源与环境学院, 福州 350002; 2福建农林大学生命科学学院, 福州 350002
  • 收稿日期:2007-05-31 修回日期:1900-01-01 出版日期:2008-02-21 发布日期:2008-02-21

Responses of Arabidopsis thaliana to oxidative stress induced by polycyclic aromatic hydrocarbon fluoranthene.

LIU Hong1; YE Yuan-bei1; CUI Bo2; ZHENG Li-min1; HUANG Yan-he1; WANG Zong-hua2   

  1. 1College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 2College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China
  • Received:2007-05-31 Revised:1900-01-01 Online:2008-02-21 Published:2008-02-21

摘要: 选用模式植物拟南芥为材料,以四环的多环芳烃(PAHs)荧蒽为研究对象,从植物对非生物胁迫响应紧密相关的抗氧化酶及膜保护系统的变化入手,研究了植物对多环芳烃胁迫的生理响应.结果表明:荧蒽胁迫下拟南芥经历了氧化胁迫和膜脂过氧化过程.0.75 mmol·L-1的荧蒽使拟南芥光合作用过程受到抑制;1.00 mmol·L-1的荧蒽使拟南芥丙二醛(MDA)含量极显著增加, 抗坏血酸过氧化物酶(APX)活性极显著下降, 膜脂过氧化作用加剧,1.25 mmol·L-1的荧蒽使拟南芥过氧化物酶(POD)活性极显著下降,H2O2在细胞内累积,拟南芥明显受害.

关键词: 车流量, 城市绿地系统, 叶面积指数, CO2浓度, 稳定碳同位素

Abstract: With the seedlings of model plant Arabidopsis thaliana and the polycyclic aromatic hydrocarbon fluoranthene as test materials, this paper studied the physiological responses of plants to polycyclic aromatic hydrocarbons from the aspects of antioxidases and membrane protection system that are tightly related to abiotic stresses. The results showed that under fluoranthene stress, the seedlings experienced oxidative stress and membranelipid peroxidization. At 0.75 mmol·L-1 of fluoranthene, the photosynthetic process was inhibited; at 1.00 mmol·L-1of fluoranthene, the malonaldehydic acid (MDA) content increased and the ascorbate peroxidase (APX) activity decreased significantly, indicating an accelerated membrane-lipid peroxidization; and at 1.25 mmol·L-1 of fluoranthene, the peroxidase (POD) activity decreased significantly and H2O2 had a high accumulation, making the seedlings injured severely.

Key words: CO2 concentration, traffic volume, stable carbon isotope, urban green space system, leaf area index