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应用生态学报 ›› 2020, Vol. 31 ›› Issue (9): 3023-3032.doi: 10.13287/j.1001-9332.202009.028

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H2S作为下游信号参与SA对低温弱光下黄瓜幼苗光合作用的调控

潘东云, 付鑫, 张晓伟, 刘丰娇, 毕焕改, 艾希珍*   

  1. 山东农业大学园艺科学与工程学院/作物生物学国家重点实验室/农业部黄淮地区园艺作物生物学与种质创制重点开放实验室/山东果蔬优质高效生产协同创新中心, 山东泰安 271018
  • 收稿日期:2020-03-13 接受日期:2020-06-28 出版日期:2020-09-15 发布日期:2021-03-15
  • 通讯作者: * E-mail: axz@sdau.edu.cn
  • 作者简介:潘东云, 女, 1994年生, 硕士研究生。主要从事黄瓜耐冷性研究。E-mail: pdy19940429@163.com
  • 基金资助:
    国家自然科学基金项目(31872155,31572170)、山东省重大科技创新工程项目(2019JZZY010715)、山东省现代农业产业技术体系建设专项(SDAIT-05-10)和“双一流”奖补资金项目(SYL2017YSTD06)资助

Hydrogen sulfide acted as a downstream signal was involved in the regulation of salicylic acid on photosynthesis of cucumber seedlings under low temperature and low light intensity

PAN Dong-yun, FU Xin, ZHANG Xiao-wei, LIU Feng-jiao, BI Huan-gai, AI Xi-zhen*   

  1. College of Horticulture Science and Engineering, Shandong Agricultural University/State Key Laboratory of Crop Biology/Key Laboratory of Horticultural Crop Biology and Germplasm Innovation in Huanghuai Region, Ministry of Agriculture/Shandong Collaborative Innovation Center of Fruit & Vegetable Qua-lity and Efficient Production, Tai’an 271018, Shandong, China
  • Received:2020-03-13 Accepted:2020-06-28 Online:2020-09-15 Published:2021-03-15
  • Contact: * E-mail: axz@sdau.edu.cn
  • Supported by:
    the National Natural Science Foundation of China (31872155, 31572170), the Major Scientific Innovation Project of Shandong Province (2019JZZY010715), the Modern Agricultural Industry Technology System in Shandong Province (SDAIT-05-10), and the Fund of Shandong “Double Tops” Program (SYL2017YSTD06).

摘要: 水杨酸(SA)和硫化氢(H2S)在调控非生物胁迫下植物生长发育和生理代谢中均起着非常重要的作用,但二者作为信号分子在调控低温弱光下黄瓜光合作用中的互作关系还不清楚。本试验以黄瓜幼苗为试材,分别用SA和硫氢化钠(NaHS,H2S供体)及其清除剂或抑制剂喷撒叶面,以适宜温光下去离子水处理为对照(CK),研究低温(8 ℃/5 ℃,昼/夜)弱光(100 μmol·m-2·s-1)下SA和H2S对黄瓜幼苗光合作用的调控及互作关系。结果表明: SA可明显增强L-/D-半胱氨酸脱巯基酶(LCD、DCD)活性及其mRNA表达,促进内源H2S产生;NaHS对苯丙氨酸解氨酶和异分支酸合成酶活性、mRNA表达量及内源SA含量影响不大。SA和NaHS可使低温弱光下黄瓜幼苗的光合速率、气孔导度和蒸腾速率明显提高,胞间CO2浓度显著降低;同时增强核酮糖-1,5-二磷酸羧化酶、Rubisco活化酶、景天庚酮糖-1,7-二磷酸酯酶和果糖-1,6-二磷酸醛缩酶活性及其mRNA表达,促进光合碳同化;提高光下PSⅡ实际光化学效率和暗下PSⅡ最大光化学效率,从而减轻低温弱光胁迫对黄瓜幼苗的光合机构的损伤和生长量的影响。H2S清除剂次牛磺酸(HT)可使SA对低温弱光下黄瓜幼苗的光合作用和生长促进效应明显减弱,而SA抑制剂多效唑和氨基茚磷酸对H2S诱导的黄瓜幼苗光合机构对低温弱光的耐受性无显著影响,说明H2S作为SA的下游信号,参与调控低温弱光下黄瓜幼苗的光合作用。

关键词: 光合作用, 低温光抑制, 水杨酸, 硫化氢, 黄瓜

Abstract: Both salicylic acid (SA) and hydrogen sulfide (H2S) play an important role in regulating plant growth and development and physiological metabolism under abiotic stresses. As signal molecules, the interaction between them in regulating cucumber photosynthesis under low temperature and low light is still unclear. Here, we examined the regulation and interaction of SA and H2S on photosynthesis in cucumber seedlings under low temperature (8 ℃/5 ℃, day/night) and low light (100 μmol·m-2·s-1). Seedlings were foliar-sprayed with SA, sodium sulfide (NaHS, H2S donor), and their scavenger or biosynthesis inhibitors, respectively. Seedlings treated with deionized water at suitable temperature and light condition were used as the control. The results showed that SA increased the L-/D-cysteine desulfhydrase (LCD, DCD) activities and relative mRNA expression, and consequently promoted the endogenous H2S production. However, NaHS did not affect the activities and gene expressions of phenylalnine ammonialyase and isochorismate and endogenous SA level. Compared with the H2O-treated seedlings under low temperature and low light, SA- and NaHS-treated seedlings showed an increase in the photosynthetic rate, stomatal conductance and transpiration rate, while a decrease in intercellular CO2 concentration. SA and NaHS increased the CO2 assimilation, which mainly attributed to the increases in the activities of the ribulose-1, 5-bisphosphate carboxylase, rubisco activase, sedoheptulose-1, 7-bisphosphatase and fructose-1, 6-bisphosphatase, as well as their mRNA expression. Meanwhile, SA and NaHS improved the actual photochemical efficiency and maximal photochemical efficiency of PSII, and therefore alleviated the damage in photosynthetic apparatus and negative effect on growth from low temperature and low light stress. The SA-induced higher photosynthesis and growth in stressed seedlings were suppressed by addition of H2S scavenger hypotaurine. However, the H2S-induced tolerance of photosynthetic apparatus to low temperature and low light was not affected by SA biosynthesis inhibitor paclobutrazol and 2-aminoindan-2-phosphonic acid. Our results suggested that H2S, as a downstream signal of SA, was involved in regulating photosynthesis in cucumber seedlings under low temperature and low light.

Key words: photosynthesis, chilling-induced photoinhibition, salicylic acid, hydrogen sulfide, cucumber