1,2,4-三氯苯胁迫对萌发大豆种子中活性氧的影响

摘要/Abstract

摘要： 以盆栽法研究了不同浓度1,2,4三氯苯(TCB)胁迫对萌发大豆种子中活性氧代谢的影响.结果表明,100～300μg·g^-1TCB胁迫初期(1～3天)促使萌发大豆种子呼吸强度升高及其峰值提前出现,超氧阴离子自由基(O₂^-)及过氧化氢(H₂O₂)的积累显著增加,同时伴随丙二醛(MDA)含量升高,显示发生膜脂质过氧化作用.TCB胁迫1～6天使活性氧清除酶功能紊乱,其中过氧化物酶(POD)活性升高,超氧化物岐化酶(SOD)活性开始上升后转为下降.在萌发大豆种子受TCB胁迫伤害过程中,活性氧代谢失衡造成的膜脂质过氧化将起着重要作用.

关键词: 1,2,4-三氯苯胁迫, 大豆种子, 活性氧, 膜脂质过氧化, 抗氧化酶, 有机物沟埋还田, 玉米, 灌水量, 光合特性, 干物质生产

Abstract: Pot culture experiment on the effects of 1,2,4 trichlorobenzene (TCB) stress on active oxygen metabolism of germinated soybean seeds indicated that during the beginning (1st～3rd day) of 100～300 μg·g^-1 TCB treatment,the respiratory peak appeared earlier,and the respiratory rate,the accumulation of superoxide radical (O₂^-) and H₂O₂,as well as the malondialdehyde (MDA) content were all enhanced. During 1～6 days of TCBstress,peroxidase (POD) activity gradually increased,and superoxide dismutase (SOD) activity increased firstly,and decreased afterward. These results suggested that the injury effects of TCBstress on germinated soybean seeds were achieved by excessive accumulation of active oxygen resulted from the degradation of active oxygen scavenging enzyme system.

Key words: trichlorobenzene stress, Soybean seeds, Active oxygen, Membrane-lipid peroxidation, Antioxidant enzymes, dry matter production, ditch-buried organic matter, irrigation amount, maize, photosynthetic characteristics

中图分类号:

X173

刘宛, 孙铁珩, 李培军, 周启星, 梁文举, 台培东, 许华夏, 张海荣. 1,2,4-三氯苯胁迫对萌发大豆种子中活性氧的影响[J]. 应用生态学报, 2002, (12): 1655-1658.

LIU Wan, SUN Tieheng, LI Peijun, ZHOU Qixing, LIANG Wenqu, TAI Peidong, XU Huaxia, ZHANG Hairong . Effects of 1, 2, 4-trichlorobenzene stress on active oxygen in germinated soybean seeds[J]. Chinese Journal of Applied Ecology, 2002, (12): 1655-1658.

参考文献

[1] Chris B,Marc VH,et al.1992.Ⅰ.Superoxide dismutase and stress tolerance.Annu Rev Plant Physiol Plant Mol Biol,43:83～87
[2] Chen W-L(陈文利),Xu L-L(徐朗莱).1999.Changes of hydrogen peroxide accumulation and hydrogen peroxide-scavenging enzyme activities in the leaves of two barley species under salt stress.J Nanjing Agric Univ(南京农业大学学报),22(2):97～100(in Chinese)
[3] Fu A-G(傅爱根),Wang A-G(王爱国),Luo G-H(罗广华).1997.Active oxygen metabolism in soybean during germination.J Trop Subtr Bot(热带亚热带植物学报),5(4):32～38(in Chinese)
[4] Fridorich I.1989.The biology in oxygen radical.Science,201:875～880
[5] Gan P(甘平).2001.Experiment of biodegradation of chlorobenzenes.Environ Sci(环境科学),22(3):93～96(in Chinese)
[6] Giannopolitis N,Ries SK.1977.Superoxide dismutaseⅠ.Occurrence in higher plants.Plant Physiol,59:309～314
[7] Ge X-C(葛秀春),Song F-M(宋凤鸣).2000.Active oxygen production in rice seedlings infected by Magnaporthe grisea is involved in the blast resistance.Acta Phytophysiol Sin(植物生理学报),26(3):227～231(in Chinese)
[8] Health RL,Packer L.1981.Photoperoxidation in isolated chloroplasts Ⅰ.Kinetics and stoichiometry of fatty acid and peroxidation.Archives Biochem Biophy,125:189 ～198
[9] Jita P,Brahma BP.1998.A comparison of biochemical responses to oxidative and metal stress in seedlings of barley,Hordeum vulgare L.Environ Poll,101:99～105
[10] Luo L-X(罗立新),Sun T-H(孙铁珩),Jin Y-H(靳月华).1998.Accumulation of superoxide radical in wheat leaves under Cadmium stress.Acta Sci Circ(环境科学学报),18(5):495～499(in Chinese)
[11] Nohl H,Hengner D.1978.Do mitochondria produce oxygen radicals in vivo? Eur J Biochem,82:563～571
[12] Patterson BD,Mackae EA,et al.1984.Estimation of hydrogen peroxide in plant extracts using titanium(Ⅳ).Anal Biochem,139:487～492
[13] Pang X(庞欣),Wang D-H(王东红),Peng A(彭安).2001.Effects of mercury stress on the activity of antioxidant enzymes.Environ Chem(环境化学),20(4):351～355 (in Chinese)
[14] Wang A-G(王爱国),Luo G-H(罗广华).1990.Quantitative relation between the reaction of hydroxylamine and superoxide anion radicals in plants.Plant Physiol Commun(植物生理学通讯),(6):55～57(in Chinese)
[15] Winston GW.1990.Physiological basis for free radical formation in cell:Production and defenses.In:Alscher RG,Cumming JG eds.Stress Response in Plants:Adaption and Acclimtion.New York:Wiley-Liss.57～86
[16] Yang J-R(杨居荣),et al.1995.Effects of Cd pollution on physiological and chemical indexes in plants.Agro-Environ Prot(农业环境保护),14(5):193～197(in Chinese)
[17] Zhang L-S(张丽珊).1992.Purification effect of slow rate land treatment system on organic pollutants in municipal wastewater from Shenyang.Chin J Appl Ecol(应用生态学报),3(4):355～363(in Chinese)
[18] Zhu G-L(朱广廉).1990.Plant Physiological Experimentation.Beijing:Peking University Press.51～53 (in Chinese)
[19] Zhang Z-L(张志良).1990.Guide for Plant Physiological Experimentation.Beijing:Higher Education Press.133～135 (in Chinese)
[20] Zhu X-Z(朱雪竹),Dong B(董斌),Xie Y-F(谢翼飞),et al.2001.Effects of Al speciation on the antioxidant system in wheat.Environ Chem(环境化学),20(2):119～123 (in Chinese)
[21] Zhu J-M(朱锦懋),Chen Y-Q(陈由强).2001.A quantitative analysis on the osmotic stress on active oxygen damage in varieties of peanut.J Plant Resour Environ(植物资源与环境学报),10(2):14～20(in Chinese)