季节性温度变化对CANON型潮汐流人工湿地脱氮的影响

doi:10.13287/j.1001-9332.202005.034

应用生态学报 ›› 2020, Vol. 31 ›› Issue (5): 1715-1724.doi: 10.13287/j.1001-9332.202005.034

季节性温度变化对CANON型潮汐流人工湿地脱氮的影响

钱昊, 王勇超, 孙峰, 武威, 陈乐意, 李苏青, 王振^*

安徽农业大学资源与环境学院农田生态保育与污染防控安徽省重点实验室, 合肥 230036

收稿日期:2019-10-07 出版日期:2020-05-15 发布日期:2020-05-15
通讯作者: * E-mail: zwang@ahau.edu.cn
作者简介:钱昊, 男, 1997年生, 硕士研究生. 主要从事污水生态处理与回用研究. E-mail: haoqianahau@163.com
基金资助:
国家自然科学基金项目(51508002)、安徽省重点研究与开发计划项目(201834040011)和安徽省高校优秀青年人才支持计划项目(gxyqZD2017016)资助

Effects of seasonal temperature variation on nitrogen removal from a tidal flow constructed wetland system with CANON process

QIAN Hao, WANG Yong-chao, SUN Feng, WU Wei, CHEN Le-yi, LI Su-qing, WANG Zhen^*

Anhui Province Key Laboratory of Farmland Ecological Conservation and Pollution Prevention, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China

Received:2019-10-07 Online:2020-05-15 Published:2020-05-15
Contact: * E-mail: zwang@ahau.edu.cn
Supported by:
This work was supported by the National Natural Science Foundation of China (51508002), the Key Research and Development Program of Anhui Province (201834040011), and the Program for Outstanding Young Talents in Colleges and Universities of Anhui Province (gxyqZD2017016).

摘要/Abstract

摘要： 探究了温度的季节性变化对基于亚硝化的全程自养脱氮(CANON)型潮汐流人工湿地(TFCW)脱氮性能及其微生物特性的影响。CANON型TFCW中的脱氮微生物群落在温度的季节性变化下会发生不同程度的改变,其脱氮途径及性能随之会出现周期性的波动。填料层温度在20.0 ℃以上时对TFCW脱氮性能及其中的优势脱氮菌群无显著影响,CANON作用是系统脱氮的主要途径。当填料层温度低于20.0 ℃时,厌氧氨氧化菌丰度与活性显著降低,在9.3~20.0 ℃时,亚硝酸盐氧化菌(NOB)的增殖及其活性的提高使TFCW中脱氮的主要途径由CANON作用演替为硝化/反硝化作用,系统对总氮(TN)的去除率仅为(34.8±13.0)%;在2.2~9.0 ℃时,TFCW中的厌氧氨氧化菌在受到抑制的同时仍保持着对NOB和反硝化菌群的相对竞争优势,系统脱氮重新依赖于CANON作用,其对TN的去除率为(54.8±4.8)%。该研究可为CANON型TFCW工艺的优化及工程化应用提供参考。

关键词: 基于亚硝化的全程自养脱氮(CANON), 潮汐流人工湿地(TFCW), 厌氧氨氧化(ANAMMOX), 温度, 脱氮

Abstract: We investigated the effects of seasonal temperature variation on the treatment perfor-mance and underlying mechanisms of nitrogen transformation in a tidal flow constructed wetland (TFCW) with the complete autotrophic nitrogen removal over nitrite (CANON) process. Different temperatures resulted in periodical variations in nitrogen transformation pathways and removal performance of the TFCW with CANON process, which was mainly due to the changes of dominant bacterial communities for nitrogen removal in the system. When temperature was higher than 20.0 ℃, nitrogen transformation and associated microbial characteristics in the TFCW were significantly affected, and the CANON process remained to be the principal pathway for nitrogen removal. The abundance and activity of anammox bacteria experienced different degrees of reduction when temperature dropped below 20.0 ℃. At the temperature of 9.3-20.0 ℃, the proliferation and increased activities of nitrite oxidizing bacteria (NOB) made the nitrification/denitrification process instead of the CANON process became the primary total nitrogen (TN) removal route in the TFCW, and the TN removal efficiency of the system declined to 34.8%±13.0%. Under the temperature range of 2.2-9.0 ℃, anammox bacteria, which was inhibited at the low temperatures, presented competitive advantage in comparison with NOB and denitrifiers, resulting that nitrogen removal in the TFCW relied on the CANON process again. Correspondingly, nitrogen removal rate of the system was 54.8%±4.8%. This study was conductive to the optimization of the TFCW with CANON process, as well as its engineering application.

Key words: completely autotrophic nitrogen removal over nitrite (CANON), tidal flow constructed wetland (TFCW), anaerobic ammonia oxidation (ANAMMOX), temperature, nitrogen removal

钱昊, 王勇超, 孙峰, 武威, 陈乐意, 李苏青, 王振. 季节性温度变化对CANON型潮汐流人工湿地脱氮的影响[J]. 应用生态学报, 2020, 31(5): 1715-1724.

QIAN Hao, WANG Yong-chao, SUN Feng, WU Wei, CHEN Le-yi, LI Su-qing, WANG Zhen. Effects of seasonal temperature variation on nitrogen removal from a tidal flow constructed wetland system with CANON process[J]. Chinese Journal of Applied Ecology, 2020, 31(5): 1715-1724.

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季节性温度变化对CANON型潮汐流人工湿地脱氮的影响

Effects of seasonal temperature variation on nitrogen removal from a tidal flow constructed wetland system with CANON process

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