芦芽山华北落叶松林不同深度土壤原生动物群落分布格局及驱动机制

doi:10.13287/j.1001-9332.202305.031

应用生态学报 ›› 2023, Vol. 34 ›› Issue (5): 1395-1403.doi: 10.13287/j.1001-9332.202305.031

芦芽山华北落叶松林不同深度土壤原生动物群落分布格局及驱动机制

任倩茹¹, 毛晓雅¹, 齐晓君¹, 刘晋仙¹, 贾彤¹, 吴铁航², 柴宝峰^1*

¹山西大学黄土高原研究所, 黄土高原生态恢复山西省重点实验室, 太原 030006;
²佐治亚州南部大学生物系, 斯泰茨伯勒GA 30460-8042, 美国

收稿日期:2022-10-18 接受日期:2023-03-01 出版日期:2023-05-15 发布日期:2023-11-15
通讯作者: *E-mail: bfchai@sxu.edu.cn
作者简介:任倩茹, 女, 1999年生, 硕士研究生。主要从事土壤微生物生态学研究。E-mail: 384892570@qq.com
基金资助:
山西省第十二批百人计划项目(2020)、中央引导地方科技发展基金项目(YDZJSX2022B001)和国家自然科学基金项目(31772450)

Distribution patterns and driving mechanism of soil protozoan community at the different depths of Larix principis-chinensis forest in the Luya Mountain, China

REN Qianru¹, MAO Xiaoya¹, QI Xiaojun¹, LIU Jinxian¹, JIA Tong¹, WU Tiehang², CHAI Baofeng^1*

¹Shanxi Key Laboratory of Ecological Restoration on Loess Plateau, Institute of Loess Plateau, Shanxi University, Taiyuan 030006, China;
²Department of Biology, Georgia Southern University, Statesboro GA 30460-8042, USA

Received:2022-10-18 Accepted:2023-03-01 Online:2023-05-15 Published:2023-11-15

摘要/Abstract

摘要： 为揭示亚高山森林生态系统土壤原生动物群落的构建机制,本文利用Illumina Miseq高通量测序技术,以芦芽山亚高山华北落叶松林土壤剖面的6个分层(枯枝落叶层、腐殖质层、0～10、10～20、20～40和40～80 cm)原生动物群落为研究对象,分析了其组成和多样性及驱动因素。结果表明: 土壤剖面中原生动物分属于 8个界21个门57个纲114个目206个科335个属。主要的优势门有5个(相对丰度>1%),优势科有10个(相对丰度>5%)。α多样性沿土壤深度的增加显著降低。主坐标分析表明,原生动物群落空间结构在土壤不同深度梯度上存在显著差异。冗余分析表明,土壤pH和含水量是决定土壤剖面原生动物群落结构的重要因子。零模型分析证实,异质选择主导了原生动物群落构建过程。分子生态网络分析显示,土壤原生动物群落的复杂性随土壤深度的增加不断降低。研究结果可为亚高山森林生态系统土壤微生物群落的构建机制研究提供数据支持。

关键词: 原生动物, 群落多样性, 华北落叶松, 土壤剖面, 群落构建

Abstract: To reveal the assembly mechanisms of soil protozoan community in subalpine forest ecosystems, we analyzed the composition and diversity of protozoan communities and their drivers at the six strata (the litter profile, humus profile, 0-10 cm, 10-20 cm, 20-40 cm and 40-80 cm) of soil profiles in subalpine Larix principis-rupprechtii forest in Luya Mountain using Illumina Miseq high-throughput sequencing technology. The results showed that protozoa in the soil profiles belonged to 335 genera, 206 families, 114 orders, 57 classes, 21 phyla, and 8 kingdoms. There were five dominant phyla (relative abundance >1%) and 10 dominant families (relative abundance >5%). The α diversity decreased significantly with increasing soil depth. Results of PCoA analysis showed that the spatial composition and structure of protozoan community differed significantly across soil depths. The results of RDA analysis showed that soil pH and soil water content were important factors driving protozoan community structure across soil profile. Null model analysis suggested that the heterogeneous selection dominated the processes of protozoan community assemblage. Molecular ecological network analysis revealed that the complexity of soil proto-zoan communities decreased continuously with increasing depth. These results elucidate the assembly mechanism of soil microbial community in subalpine forest ecosystem.

Key words: protozoa, community diversity, Larix principis-rupprechtii, soil profile, community assemblage

任倩茹, 毛晓雅, 齐晓君, 刘晋仙, 贾彤, 吴铁航, 柴宝峰. 芦芽山华北落叶松林不同深度土壤原生动物群落分布格局及驱动机制[J]. 应用生态学报, 2023, 34(5): 1395-1403.

REN Qianru, MAO Xiaoya, QI Xiaojun, LIU Jinxian, JIA Tong, WU Tiehang, CHAI Baofeng. Distribution patterns and driving mechanism of soil protozoan community at the different depths of Larix principis-chinensis forest in the Luya Mountain, China[J]. Chinese Journal of Applied Ecology, 2023, 34(5): 1395-1403.

参考文献

[1] Geisen S, Mitchell EAD, Adl S, et al. Soil protists: A fertile frontier in soil biology research. FEMS Microbio-logy Reviews, 2018, 42: 293-323
[2] Xiong W, Song Y, Yang K, et al. Rhizosphere protists are key determinants of plant health. Microbiome, 2020, 8: 27-35
[3] Ceja-Navarro JA, Wang Y, Ning DL, et al. Protist diversity and community complexity in the rhizosphere of switchgrass are dynamic as plants develop. Microbiome, 2021, 9: 96-113
[4] Grossmann L, Jensen M, Heider D, et al. Protistan community analysis: Key findings of a large-scale mole-cular sampling. The ISME Journal, 2016, 10: 2269-2279
[5] Howe AT, Bass D, Vickerman K, et al. Phylogeny, taxonomy, and astounding genetic diversity of Glissomo-nadida ord. Nov., the dominant gliding zooflagellates in soil (Protozoa: Cercozoa). Protist, 2009, 160: 159-189
[6] Khanipour Roshan S, Dumack K, Bonkowski M, et al. Taxonomic and functional diversity of heterotrophic protists (Cercozoa and Endomyxa) from biological soil crusts. Microorganisms, 2021, 9: 205-318
[7] Ji L, Shen FY, Liu Y, et al. Contrasting altitudinal patterns and co-occurrence networks of soil bacterial and fungal communities along soil depths in the cold-tempe-rate montane forests of China. Catena, 2022, 209: 105844-105856
[8] 韦中, 宋宇琦, 熊武, 等. 土壤原生动物——研究方法及其在土传病害防控中的作用. 土壤学报, 2021, 58: 14-22
[9] Bates ST, Clemente JC, Flores GE, et al. Global biogeo-graphy of highly diverse protistan communities in soil. The ISME Journal, 2013, 7: 652-659
[10] 刘株秀, 刘俊杰, 金剑, 等. 土壤剖面微生物群落分布规律研究进展. 土壤与作物, 2022, 11(2): 129-138
[11] Islam W, Saqib HSA, Tayyab M, et al. Natural forest chronosequence maintains better soil fertility indicators and assemblage of total belowground soil biota than Chinese fir monoculture in subtropical ecosystem. Journal of Cleaner Production, 2022, 334: 130228-130240
[12] 罗正明, 刘晋仙, 周妍英, 等. 亚高山草地土壤原生生物群落结构和多样性海拔分布格局. 生态学报, 2021, 41(7): 2783-2793
[13] Chen Y, Yang X, Fu W, et al. Conversion of natural grassland to cropland alters microbial community assembly across Northern China. Environmental Microbiology, 2022, 24: 5630-5642
[14] Xiong W, Jousset A, Li R, et al. A global overview of the trophic structure within microbiomes across ecosystems. Environment International, 2021, 151: 106438-106447
[15] 陈志豪, 梁雪, 李永春, 等. 不同施肥模式对雷竹林土壤真菌群落特征的影响. 应用生态学报, 2017, 28(4): 1168-1176
[16] 陈瑞蕊, 张建伟, 董洋, 等. 盐度对滨海土壤细菌多样性和群落构建过程的影响. 应用生态学报, 2021, 32(5): 1816-1824
[17] Stegen JC, Lin X, Fredrickson JK, et al. Quantifying community assembly processes and identifying features that impose them. The ISME Journal, 2013, 7: 2069-2079
[18] Scharroba A, Dibbern D, Hünninghaus M, et al. Effects of resource availability and quality on the structure of the micro-food web of an arable soil across depth. Soil Bio-logy and Biochemistry, 2012, 50: 1-11
[19] Fiore-Donno AM, Richter-Heitmann T, Bonkowski M. Contrasting responses of protistan plant parasites and phagotrophs to ecosystems, land management and soil properties. Frontiers in Microbiology, 2020, 11: 1823-1835
[20] Degrune F, Dumack K, Fiore-Donno AM, et al. Distinct communities of Cercozoa at different soil depths in a temperate agricultural field. FEMS Microbiology Eco-logy, 2019, 95, doi: 10.1093/femsec/fiz041
[21] Bass D, Tikhonenkov DV, Foster R, et al. Rhizarian ‘novel clade 10' revealed as abundant and diverse planktonic and terrestrial flagellates, including Aquavolon n. gen. Journal of Eukaryotic Microbiology, 2018, 65: 828-842
[22] Xu R, Zhang MM, Lin HZ, et al. Response of soil protozoa to acid mine drainage in a contaminated terrace. Journal of Hazardous Materials, 2022, 421: 126790-126799
[23] Sosa-Hernández MA, Roy J, Hempel S, et al. Subsoil arbuscular mycorrhizal fungal communities in arable soil differ from those in topsoil. Soil Biology and Biochemistry, 2018, 117: 83-86
[24] Hussain S, Liu H, Liu S, et al. Distribution and assembly processes of soil fungal communities along an altitudinal gradient in Tibetan Plateau. Journal of Fungi, 2021, 7: 1082-1105
[25] Dini-Andreote F, Stegen JC, van Elsas JD, et al. Disen-tangling mechanisms that mediate the balance between stochastic and deterministic processes in microbial succession. Proceedings of the National Academy of Sciences of the United States of America, 2015, 112: E1326-E1332
[26] Du X, Deng Y, Li S, et al. Steeper spatial scaling patterns of subsoil microbiota are shaped by deterministic assembly process. Molecular Ecology, 2021, 30: 1072-1085
[27] Guo X, Feng J, Shi Z, et al. Climate warming leads to divergent succession of grassland microbial communities. Nature Climate Change, 2018, 8: 813-818
[28] Geisen S. Soil water availability strongly alters the community composition of soil protists. Pedobiologia, 2014, 57: 205-213
[29] Asiloglu R, Samuel SO, Sevilir B, et al. Biochar affects taxonomic and functional community composition of protists. Biology and Fertility of Soils, 2020, 57: 15-29
[30] Jiao S, Chen WM, Wang JL, et al. Soil microbiomes with distinct assemblies through vertical soil profiles drive the cycling of multiple nutrients in reforested ecosystems. Microbiome, 2018, 6: 146-158
[31] Carr A, Diener C, Baliga NS, et al. Use and abuse of correlation analyses in microbial ecology. The ISME Journal, 2019, 13: 2647-2655
[32] Jia T, Liang X, Guo T, et al. Bacterial community succession and influencing factors for Imperata cylindrica litter decomposition in a copper tailings area of China. Science of the Total Environment, 2022, 815: 152908-152921
[33] Brazeau HA, Schamp BS. Examining the link between competition and negative co-occurrence patterns. Oikos, 2019, 128: 1358-1366

芦芽山华北落叶松林不同深度土壤原生动物群落分布格局及驱动机制

Distribution patterns and driving mechanism of soil protozoan community at the different depths of Larix principis-chinensis forest in the Luya Mountain, China

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