短期氮添加下不同树种土壤可溶性有机质的变化及其与真菌群落的关联

doi:10.13287/j.1001-9332.202501.016

应用生态学报 ›› 2025, Vol. 36 ›› Issue (1): 67-76.doi: 10.13287/j.1001-9332.202501.016

短期氮添加下不同树种土壤可溶性有机质的变化及其与真菌群落的关联

柏欣宇^1,2, 元晓春^1,2,3*, 曾泉鑫^1,2, 张晓晴^1,2, 孙浩^1,2, 张秋芳^1,2, 卢姣宏^1,2, 崔琚琰^4,5, 陈岳民^1,2

¹福建师范大学地理科学学院, 福州 350007;
²福建师范大学湿润亚热带山地生态国家重点实验室培育基地, 福州 350007;
³武夷学院旅游学院, 福建武夷山 354300;
⁴安徽大学资源与环境工程学院, 合肥 230601;
⁵铜陵学院建筑工程学院, 安徽铜陵 244061

收稿日期:2024-06-24 修回日期:2024-11-13 出版日期:2025-01-18 发布日期:2025-07-18
通讯作者: *E-mail: Yuanxc@wuyi.edu.cn
作者简介:柏欣宇, 男, 2001年生, 硕士研究生。主要从事全球变化背景下森林土壤养分循环研究。E-mail: 2592834634@qq.com
基金资助:
国家自然科学基金项目(32201532,32371846)和安徽省高校自然科学研究项目(2023AH051667)

Change of soil dissolved organic matter and its association with fungal communities after short-term nitrogen addition in different tree species

BAI Xinyu^1,2, YUAN Xiaochun^1,2,3*, ZENG Quanxin^1,2, ZHANG Xiaoqing^1,2, SUN Hao^1,2, ZHANG Qiufang^1,2, LU Jiaohong^1,2, CUI Juyan^4,5, CHEN Yuemin^1,2

¹School of Geographical Science, Fujian Normal University, Fuzhou 350007, China;
²Cultivation Base of State Key Laboratory of Subtropical Mountain Ecology, Fujian Normal University, Fuzhou 350007, China;
³College of Tourism, Wuyi University, Wu-yishan 354300, Fujian, China;
⁴School of Resources and Environmental Engineering, Anhui University, Hefei 230601, China;
⁵School of Architectural Engineering, Tongling College, Tongling 244061, Anhui, China

Received:2024-06-24 Revised:2024-11-13 Online:2025-01-18 Published:2025-07-18

摘要/Abstract

摘要： 土壤可溶性有机质(DOM)是土壤碳库的重要组成部分,其与土壤真菌群落的相互作用对土壤碳循环至关重要。本研究在黄山松和罗浮栲林中分别设置3个氮添加水平,包括对照(0 kg N·hm^-2·a^-1)、低氮(40 kg N·hm^-2·a^-1)、高氮(80 kg N·hm^-2·a^-1),并采用三维荧光与平行因子分析联用,结合高通量测序,探讨了氮添加对土壤DOM的数量和光学特性的影响及其与真菌群落的关联。结果表明: 在黄山松林中,氮添加下土壤DOM数量和光学特性以及土壤真菌Chao1指数均无显著变化;而在罗浮栲林中,氮添加下土壤可溶性有机碳含量、DOM的腐殖化指数和类腐殖质组分的相对含量降低,尤其在高氮处理下,土壤真菌群落中被孢霉门的相对丰度显著增加。冗余分析结果显示,黄山松林土壤DOM数量和光学特性主要受土壤真菌Chao1指数的影响,而罗浮栲林土壤DOM数量和光学特性的主要影响因子为真菌群落结构、β-葡糖苷酶活性和土壤pH。这说明短期氮添加下不同树种土壤DOM数量和光学特性的主要影响因子不同。网络分析发现,土壤真菌群落与DOM组分之间存在密切联系,而氮添加下土壤真菌群落与DOM组分的网络关联边减少,说明两者的相互作用被削弱。本研究可为氮沉降下森林土壤真菌群落与土壤可溶性碳库之间的联系提供新的见解。

关键词: 氮沉降, 树种, 可溶性有机质, 真菌群落, 共现网络

Abstract: Soil dissolved organic matter (DOM) is an important component of soil carbon pool, and its interaction with soil fungal communities is crucial for soil carbon cycle. We set up three nitrogen (N) addition levels, including control (0 kg N·hm^-2·a^-1), low N (40 kg N·hm^-2·a^-1), and high N (80 kg N·hm^-2·a^-1), in Pinus taiwanensis and Castanopsis faberi forests to explore the effect of N addition on the DOM quantity and optical properties, as well as the relationship between soil fungal communities and DOM by using three-dimensional fluorescence spectroscopy coupled with parallel factor analysis and high-throughput sequencing. The results showed that N addition did not significantly change the quantity or optical properties of soil DOM and Chao1 index in P. taiwanensis forest. In contrast, N addition, especially the high addition rate, significantly reduced the content of soil dissolved organic carbon, humification index, and relative content of humic-like components of DOM in C. faberi forest. Additionally, the relative abundance of Mortierellomycota in the fungal community significantly increased after N addition. Redundancy analysis indicated that soil DOM content and optical properties in P. taiwanensis forest were mainly influenced by Chao1 index after N addition. In contrast, soil fungal community structure, β-glucosidase activity, and pH were the main factors influencing DOM content and optical properties in C. faberi forests. This suggested that the key factors affecting soil DOM quantity and optical properties after short-term N addition depended on tree species. Furthermore, network analysis demonstrated a close relationship between soil fungal communities and DOM components. Fewer associative edges were observed, suggesting that the association between soil fungal communities and DOM components was weakened by N addition. Overall, this study provided novel insights into the relationship between fungal communities and dissolved organic carbon pools in forest soils with N addition.

Key words: nitrogen deposition, tree species, dissolved organic matter, fungal community, co-occurrence network

柏欣宇, 元晓春, 曾泉鑫, 张晓晴, 孙浩, 张秋芳, 卢姣宏, 崔琚琰, 陈岳民. 短期氮添加下不同树种土壤可溶性有机质的变化及其与真菌群落的关联[J]. 应用生态学报, 2025, 36(1): 67-76.

BAI Xinyu, YUAN Xiaochun, ZENG Quanxin, ZHANG Xiaoqing, SUN Hao, ZHANG Qiufang, LU Jiaohong, CUI Juyan, CHEN Yuemin. Change of soil dissolved organic matter and its association with fungal communities after short-term nitrogen addition in different tree species[J]. Chinese Journal of Applied Ecology, 2025, 36(1): 67-76.

参考文献

[1] Ackerman D, Millet DB, Chen X. Global estimates of inorganic nitrogen deposition across four decades. Global Biogeochemical Cycles, 2019, 33: 100-107
[2] Wang R, Goll D, Balkanski Y, et al. Global forest carbon uptake due to nitrogen and phosphorus deposition from 1850 to 2100. Global Change Biology, 2017, 23: 4854-4872
[3] Yu GR, Jia YL, He NP, et al. Stabilization of atmospheric nitrogen deposition in China over the past decade. Nature Geoscience, 2019, 12: 424-429
[4] Du EZ, De Vries W. Nitrogen-induced new net primary production and carbon sequestration in global forests. Environmental Pollution, 2018, 242: 1476-1487
[5] Gilliam FS, Burns DA, Driscoll CT, et al. Decreased atmospheric nitrogen deposition in eastern North America: Predicted responses of forest ecosystems. Environmental Pollution, 2019, 244: 560-574
[6] Forsmark B, Nordin A, Maaroufi NI, et al. Low and high nitrogen deposition rates in northern coniferous forests have different impacts on aboveground litter production, soil respiration, and soil carbon stocks. Ecosystems, 2020, 23: 1423-1436
[7] 张政, 蔡小真, 唐偲頔, 等. 可溶性有机质输入对杉木人工林表层土壤有机碳矿化的激发效应. 生态学报, 2017, 37(22): 7660-7667
[8] Minor EC, Oyler AR. Dissolved organic matter in large lakes: A key but understudied component of the carbon cycle. Biogeochemistry, 2023, 164: 295-318
[9] Hu A, Choi M, Tanentzap AJ, et al. Ecological networks of dissolved organic matter and microorganisms under global change. Nature Communications, 2022, 13: 3600
[10] Qiu QY, Wu LF, Ouyang Z, et al. Effects of plant-derived dissolved organic matter (DOM) on soil CO₂ and N₂O emissions and soil carbon and nitrogen sequestrations. Applied Soil Ecology, 2015, 96: 122-130
[11] Yuan XC, Cui JY, Wu LZ, et al. Relationship between soil bacterial communities and dissolved organic matter in a subtropical Pinus taiwanensis forest after short-term nitrogen addition. Forest Ecology and Management, 2022, 512: 120165
[12] Pregitzer KS, Zak DR, Burton AJ, et al. Chronic nitrate additions dramatically increase the export of carbon and nitrogen from northern hardwood ecosystems. Biogeochemistry, 2004, 68: 179-197
[13] Hobbie SE. Plant species effects on nutrient cycling: Revisiting litter feedbacks. Trends in Ecology and Evolution, 2015, 30: 357-363
[14] Xu PD, Shi L, Yang X, et al. Influence mechanisms of N addition on the concentration of soil dissolved organic matter in China. Land Degradation & Development, 2023, 34: 4690-4698
[15] Wu XQ, Wu LY, Liu YN, et al. Microbial interactions with dissolved organic matter drive carbon dynamics and community succession. Frontiers in Microbiology, 2018, 9: 359717
[16] Boberg JB, Ihrmark K, Lindahl BD. Decomposing capacity of fungi commonly detected in Pinus sylvestris needle litter. Fungal Ecology, 2011, 4: 110-114
[17] Rezacova V, Hrselova H, Gryndlerova H, et al. Modifications of degradation-resistant soil organic matter by soil saprobic microfungi. Soil Biology and Biochemistry, 2006, 38: 2292-2299
[18] Li YP, Wang SR, Zhang L, et al. Composition and spectroscopic characteristics of dissolved organic matter extracted from the sediment of Erhai Lake in China. Journal of Soils and Sediments, 2014, 14: 1599-1611
[19] Yang Y, Chen XL, Liu LX, et al. Nitrogen fertilization weakens the linkage between soil carbon and microbial diversity: A global meta-analysis. Global Change Biology, 2022, 28: 6446-6461
[20] 元晓春, 崔琚琰, 林开淼, 等. 黄山松土壤可溶性有机质对氮添加的响应及其与细菌群落的关联. 应用生态学报, 2021, 32(11): 4085-4094
[21] Su SJ, Liu JF, He ZS, et al. Ecological species groups and interspecific association of dominant tree species in Daiyun Mountain National Nature Reserve. Journal of Mountain Science, 2015, 12: 637-646
[22] 张晓晴, 曾泉鑫, 元晓春, 等. 氮添加诱导的磷限制改变了亚热带黄山松林土壤微生物群落结构. 应用生态学报, 2023, 34(1): 203-212
[23] 戴辉, 曾泉鑫, 周嘉聪, 等. 罗浮栲林土壤微生物碳利用效率对短期氮添加的响应. 应用生态学报, 2022, 33(10): 2611-2618
[24] Shi YL, Cui SH, Ju XT, et al. Impacts of reactive nitrogen on climate change in China. Scientific Reports, 2015, 5: 8118
[25] 苏涛, 司美茹, 王朝辉, 等. 土壤矿质氮分析方法的影响因素研究. 农业环境科学学报, 2005, 24(6): 1238-1242
[26] Martin JK, Correll RL. Measurement of microbial biomass phosphorus in rhizosphere soil. Plant and Soil, 1989, 113: 213-221
[27] 林开淼, 元晓春, 曾泉鑫, 等. 氮添加对戴云山黄山松林土壤有机氮解聚酶活性的影响及其调控因素. 生态学报, 2023, 43(16): 6550-6559
[28] Saiya-Cork KR, Sinsabaugh RL, Zak DR. The effects of long term nitrogen deposition on extracellular enzyme activity in an Acer saccharum forest soil. Soil Biology and Biochemistry, 2002, 34: 1309-1315
[29] Bu XL, Wang LM, Ma WB, et al. Spectroscopic cha-racterization of hot-water extractable organic matter from soils under four different vegetation types along an elevation gradient in the Wuyi Mountains. Geoderma, 2010, 159: 139-146
[30] 元晓春, 陈岳民, 袁硕, 等. 氮沉降对杉木人工幼林土壤溶液可溶性有机物质浓度及光谱学特征的影响. 应用生态学报, 2017, 28(1): 1-11
[31] Yuan XC, Si YT, Lin WS, et al. Effects of short-term warming and nitrogen addition on the quantity and quality of dissolved organic matter in a subtropical Cunninghamia lanceolata plantation. PLoS One, 2018, 13: e0191403
[32] 曾阿莹, 胡伟芳, 张林海, 等. 盐度和淹水程度对短叶茳芏枯落物分解初期DOM含量及其组成结构的影响. 生态学报, 2020, 40(8): 2751-2762
[33] Lu M, Zhou XH, Luo YQ, et al. Minor stimulation of soil carbon storage by nitrogen addition: A meta-analysis. Agriculture, Ecosystems & Environment, 2011, 140: 234-244
[34] Ma K, Li YY, Liu X, et al. Bacteria rather than fungi mediate the chemodiversity of dissolved organic matter in a mudflat intertidal zone. Science of the Total Environment, 2023, 893: 164835
[35] Li Y, Heal K, Wang SZ, et al. Chemodiversity of soil dissolved organic matter and its association with soil microbial communities along a chronosequence of Chinese fir monoculture plantations. Frontiers in Microbiology, 2021, 12: 729344
[36] Collado S, Oulego P, Suarez-Iglesias O, et al. Biodegradation of dissolved humic substances by fungi. Applied Microbiology and Biotechnology, 2018, 102: 3497-3511
[37] Shen J, Liang ZY, Kuzyakov Y, et al. Dissolved organic matter defines microbial communities during initial soil formation after deglaciation. Science of the Total Environment, 2023, 878: 163171
[38] Li S, Zhang SR, Pu YL, et al. Dynamics of soil labile organic carbon fractions and C-cycle enzyme activities under straw mulch in Chengdu Plain. Soil and Tillage Research, 2016, 155: 289-297
[39] Fan YX, Yang LM, Zhong XJ, et al. N addition increased microbial residual carbon by altering soil P availability and microbial composition in a subtropical Castanopsis forest. Geoderma, 2020, 375: 114470
[40] Zhang YL, Heal KV, Shi MJ, et al. Decreasing molecular diversity of soil dissolved organic matter related to microbial community along an alpine elevation gradient. Science of the Total Environment, 2022, 818: 151823
[41] Zuo YW, Yang LX, Wang Q, et al. Divergent fungal community dynamics of Thuja sutchuenensis in arid environments. Microorganisms, 2024, 12: 446
[42] Han KH, Choi WJ, Han GH, et al. Urea-nitrogen transformation and compost-nitrogen mineralization in three different soils as affected by the interaction between both nitrogen inputs. Biology and Fertility of Soils, 2004, 39: 193-199
[43] 方华军, 耿静, 程淑兰, 等. 氮磷富集对森林土壤碳截存的影响研究进展. 土壤学报, 2019, 56(1): 1-11
[44] Keeler BL, Hobbie SE, Kellogg LE. Effects of long-term nitrogen addition on microbial enzyme activity in eight forested and grassland sites: Implications for litter and soil organic matter decomposition. Ecosystems, 2009, 12: 1-15
[45] Mannisto M, Ganzert L, Tiirola M, et al. Do shifts in life strategies explain microbial community responses to increasing nitrogen in tundra soil? Soil Biology and Biochemistry, 2016, 96: 216-228
[46] Fierer N, Lauber CL, Ramirez KS, et al. Comparative metagenomic, phylogenetic and physiological analyses of soil microbial communities across nitrogen gradients. The ISME Journal, 2012, 6: 1007-1017
[47] de Vries FT, Hoffland E, van EekerenN, et al. Fungal/bacterial ratios in grasslands with contrasting nitrogen management. Soil Biology and Biochemistry, 2006, 38: 2092-2103
[48] Rousk J, Brookes PC, Baath E. Contrasting soil pH effects on fungal and bacterial growth suggest functional redundancy in carbon mineralization. Applied and Environmental Microbiology, 2009, 75: 1589-1596
[49] Nonghuloo IM, Kharbhih S, Suchiang BR, et al. Production, decomposition and nutrient contents of litter in subtropical broadleaved forest surpass those in coniferous forest, Meghalaya. Tropical Ecology, 2020, 61: 5-12

短期氮添加下不同树种土壤可溶性有机质的变化及其与真菌群落的关联

Change of soil dissolved organic matter and its association with fungal communities after short-term nitrogen addition in different tree species

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