土壤、植物样品中多环芳烃(PAHs)分析方法研究

应用生态学报 ›› 1995, Vol. 6 ›› Issue (1): 92-96.

土壤、植物样品中多环芳烃(PAHs)分析方法研究

宋玉芳, 区自清, 孙铁珩, A. Yediler, G. Lorinci, A. Kettrup

1. 中国科学院沈阳应用生态研究所, 沈阳 110015;
2. 德国环境与辐射研究中心生态化学研究所

收稿日期:1994-07-07 修回日期:1994-08-03 出版日期:1995-01-25 发布日期:1995-01-25
基金资助:
中－德生态合作(CERP)研究项目

Analytical method of polycyclic aromatic hydrocarbons (PAHs) in soil and plant samples

Song Yufang, Ou Ziqing, Sun Tieheng, A. Yediler, G. Lorinci, A. Kettrup

1. Institute of Applied Ecology, Academia Sinica, Shenyang 110015;
2. Forschungszentrum für Umwelt und Gesundheil Gm bH, Institute of Ecological Chemistry, Nurherberg, Germany D-85758

Received:1994-07-07 Revised:1994-08-03 Online:1995-01-25 Published:1995-01-25

摘要/Abstract

摘要： 土壤、植物和籽实样品分别用四氢呋喃、甲醇、乙酸乙酯以超声技术提取。提取液经旋转浓缩蒸发仪浓缩,经硅胶柱净化后。由高效液相色谱(HPLC)分离,萤光检测分析。对于土壤、植物和籽实样品,其方法回收率根据各个PAH化合物的理化性质不同分别为45.68-93.42、77.59-108.13和79.11-98.96%.结果表明,二氯甲烷、四氢呋喃适合作为土壤样品的提取剂;甲醇、乙酸乙酯分别适合于植物和籽实样品的提取剂。本分析方法简单、快速、灵敏、可靠,可用于土壤-植物系统多环芳烃的研究。

关键词: 高效液相色谱, 土壤、植物分析, 多环芳烃, 生长阶段, 土壤微生物生物量碳, 土壤理化性质, 植被恢复

Abstract: Soil, plant and seed samples are respectively extracted by tetrahydrafuran, methanol andethyl acetate ultrasonically.The extracts are evaporated by rotary evaporator, cleaned upon silica gel column and analyzed on HPLCwith fluorescence detector.The recovery ofPAHs is 45.68-93.42% for soil, 77.59-108.13% for plant, and 79.11-98.96% forseed, depending on the physico-chemical properties of individual PAHcompounds.Dichloromethane and tetrahydrafuran are the suitable extracting agents for soil, andmethanol and ethyl acetate for plant and seed.respectively.This method has the characteristics of simplicity, rapidity, sensitivity and reliance, and can be used for the study ofPAHs in soil-plant system.

Key words: Polycyclic aromatic hydrocarbons, HPLC Soil and plant analysis, vegetation restoration, soil microbial biomass carbon, soil physical-chemical property, growth stage

宋玉芳, 区自清, 孙铁珩, A. Yediler, G. Lorinci, A. Kettrup. 土壤、植物样品中多环芳烃(PAHs)分析方法研究[J]. 应用生态学报, 1995, 6(1): 92-96.

Song Yufang, Ou Ziqing, Sun Tieheng, A. Yediler, G. Lorinci, A. Kettrup. Analytical method of polycyclic aromatic hydrocarbons (PAHs) in soil and plant samples[J]. Chinese Journal of Applied Ecology, 1995, 6(1): 92-96.

参考文献

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