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甘蔗抗旱性生理生化鉴定指标

高三基;罗俊;张华;陈如凯;林彦铨   

  1. 福建农林大学农业部甘蔗生理生态与遗传改良重点开放实验室,福州 350002
  • 收稿日期:2005-06-27 修回日期:2006-03-26 出版日期:2006-06-18 发布日期:2006-06-18

Physiological and biochemical indexes of drought resistance of sugarcane (Saccharum spp.)

GAO Sanji; LUO Jun; ZHANG Hua; CHEN Rukai; LIN Yanquan   

  1. Key Laboratory of Ecophysiology & Genetic Improvement for Sugarcane of Ag
    riculture Ministry, Fujian Agriculture and Forestry University, Fuzhou 350002, China
  • Received:2005-06-27 Revised:2006-03-26 Online:2006-06-18 Published:2006-06-18

摘要: 利用因子分析和灰色关联度分析方法研究了甘蔗叶片相对含水量、膜脂过氧化代谢、活性氧代谢、光合参数及蔗茎产量性状等指标与抗旱性的关系.结果表明,干旱胁迫下甘蔗叶片的MDA含量和PMP明显提高,而RWC、SOD活性、Chl含量、Fv/FmFv/Fo、△Fv/Ft、△Fv/Fo和蔗茎单茎重(SSW)8个抗旱性指标均显著降低.SSW与其它9个生理生化指标的相关性大小依次为PMP>SOD活性>MDA含量>RWC>Fv/FoFv/Fm>Chl含量>Fv/FoFv/Ft,其中,SSW与Fv/Fo和ΔFv/Ft相关性不显著.通过因子分析将10个甘蔗抗旱性指标用4个公共因子表示,累加方差贡献率达到92.08%.因子l主要是反映光合作用特性指标对甘蔗品种抗旱性起支配作用,因子2主要是反映叶片相对含水量及活性氧代谢指标对甘蔗品种抗旱性起支配作用,因子3和因子4分别只有SSW和Chl含量有较大载荷.灰色关联度分析表明,各抗旱性生理生化指标与SSW关联密切程度依次为Fv/Fm>PMP>Fv/Fo>RWC>MDA含量>SOD活性>ΔFv/Ft>Chl含量>Fv/Fo.

关键词: 大羽藓, 垂藓, 光合作用, 光响应, 温度响应, 水分响应

Abstract: Through factor analysis and grey correlative degree analysis, this paper studied the relationships of sugarcane leaf relative water content (RWC), membrane lipid peroxidation metabolism, active oxygen metabolism, photosynthesis, and stalk yield with drought resistance of sugarcane. The results suggested that under drought stress, the plasma membrane permeability (PMP) and malondialdehyde (MDA) content were markedly increased, while RWC, superoxide dismutase (SOD) activity, chlorophyll (Chl) content, PSⅡ photochemical efficiency (Fv/Fm), PSⅡ potential activity (Fv/Fo), variable fluorescence quenching rate (ΔFv/Fo), efficiency of potential quantum conversion (ΔFv/Ft), and single stalk weight (SSW) were remarkably decreased. The correlation of SSW with test indexes was decreased in the order of PMP, SOD activity, MDA content, RWC, Fv/Fo, Fv/Fm, Chl content, ΔFv/Fo and ΔFv/Ft. Through factor analysis, the test indexes were divided into four common factors, among which, the indexes of photosynthesis played important roles in the first factor, RWC and the indexes of active oxygen metabolism dominated in the second factor, while SSW and Chl content played primary roles in the third and fourth factor, respectively. The grey correlative degree of SSW with other indexes was in the rank of Fv/Fm, PMP, Fv/Fo, RWC, MDA content, SOD activity, ΔFv/Ft, Chl content, and ΔFv/Fo.

Key words: Thuidium cymbifolium, Chrysocladium retrorsum, Photosynthesis, Light response, Temperature response, Water relation