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Bt毒素在三种矿物表面的吸附与解吸

周学永1, 2;高建保1;蔡鹏3;黄巧云3   

  1. 1武汉科技大学湖北省高温陶瓷与耐火材料重点实验室, 武汉 430081;2 天津农学院食品科学系, 天津 300384;3华中农业大学农业微生物国家重点实验室, 武汉 430070
  • 收稿日期:2007-08-15 修回日期:1900-01-01 出版日期:2008-05-20 发布日期:2008-05-20

Adsorption and desorption of Bt toxin on three kinds of minerals.

ZHOU Xue-yong1,2; GAO Jian-bao1;CAI Peng3;HUANG Qiao-yun3   

  1. 1Key Laboratory of Refractories and Ceramics of Hubei Province, Wuhan University of Science and Technology, Wuhan 430081, China;2Department of Food Science, Tianjin Agricultural College, Tianjin 300384,China;3State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China
  • Received:2007-08-15 Revised:1900-01-01 Online:2008-05-20 Published:2008-05-20

摘要: 研究了Bt库斯塔克亚种(kurstaki)毒素(65 kDa)在高岭土、针铁矿和氧化硅表面的吸附和解吸特性.结果表明:在磷酸盐缓冲体系(pH 8)中,3种矿物的等温吸附曲线均符合Langmuir方程(R2>0.9661),它们对Bt毒素的吸附顺序为:针铁矿﹥高岭土﹥二氧化硅.矿物对Bt毒素的吸附1 h就基本达到了吸附平衡.在pH 6~8范围内,针铁矿、高岭土和二氧化硅对Bt毒素的吸附量随pH值的升高而降低.10 ℃~50 ℃范围内,针铁矿和氧化硅对Bt毒素吸附量随温度升高有所下降(8.39%和47.06%),高岭土对Bt毒素吸附则略有升高(5.91%).红外光谱分析显示,Bt毒素被矿物吸附后结构仅有微小变化.被矿物吸附的Bt毒素不易被去离子水解吸,水洗3次总解吸率为28.48%~42.04%.

关键词: 稻茬小麦, 分蘖能力, 养分吸收利用, 耕作播种方式, 产量

Abstract: This paper studied the characteristics of the adsorption and desorption of Bt (Bacillus thuringiensis)toxin on goethite, kaolinite, and silica. The results showed that in phosphate buffer (pH 8), the adsorption isotherms ofBt toxin on the test minerals followed Langmuir equation (R2>0.9661), and the adsorbed amounts were in the order of goethite﹥kaolinite﹥silica. The Btoxin was easily adsorbed on the minerals, and the adsorption could reach equilibrium after 1 hour. Within the range of pH 6-8, the amounts of Bt toxin adsorbed on goethite, kaolinite and silica decreased with increasing pH; in the range of 10 ℃-50 ℃, the amounts of the toxin adsorbed on goethite and silica decreased by 8.39% and 47.06%, respectively, while that on kaolinite increased slightly (5.91%). The infrared absorption spectrum showed that there was only a minor alteration of Bt toxin after adsorption. The toxin adsorbed on the minerals was not easily desorbed by deionised water, with the desorption rate ranged from 28.48% to 42.04% after three times washing.

Key words: wheat after rice, tillage and sowing practice, soil nutrient uptake and utilization, grain yield, tillering capacity