黄土高原不同植被带人工刺槐林土壤氮磷转化酶动力学参数及其温度敏感性

doi:10.13287/j.1001-9332.202008.003

应用生态学报 ›› 2020, Vol. 31 ›› Issue (8): 2515-2522.doi: 10.13287/j.1001-9332.202008.003

黄土高原不同植被带人工刺槐林土壤氮磷转化酶动力学参数及其温度敏感性

赵子文^1,2, 王国梁^1,3, 吴阳⁴, 陈文静⁴, 李秧秧^1,3*, 刘国彬^1,3, 薛萐^1,4

¹中国科学院水利部水土保持研究所, 黄土高原土壤侵蚀与旱地农业国家重点实验室, 陕西杨凌 712100;
²中国科学院大学, 北京 100049;
³西北农林科技大学水土保持研究所, 陕西杨凌 712100;
⁴西北农林科技大学林学院, 陕西杨凌 712100

收稿日期:2019-12-30 修回日期:2020-05-25 出版日期:2020-08-15 发布日期:2021-02-15
通讯作者: * E-mail: yyli@ms.iswc.ac.cn
作者简介:赵子文, 男, 1996年生, 硕士研究生。主要从事土壤微生物生态研究。E-mail: zhaozw18@163.com
基金资助:
国家重点研发计划项目(2016YFC0501707)和国家自然科学基金项目(41771557)资助

Kinetic parameters and temperature sensitivity of soil nitrogen and phosphorus transforming enzymes in Robinia pseudoacacia plantations under different vegetation zones on the Loess Plateau, China

ZHAO Zi-wen^1,2, WANG Guo-liang^1,3, WU Yang⁴, CHEN Wen-jing⁴, LI Yang-yang^1,3*, LIU Guo-bin^1,3, XUE Sha^1,4

¹State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Institute of Soil and Water Conservation, Chinese Academy of Sciences and Ministry of Water Resources, Yangling 712100, Shaanxi, China;
²University of Chinese Academy of Sciences, Beijing 100049, China;
³Institute of Soil and Water Conservation, Northwest A&F University, Yangling 712100, Shaanxi, China;
⁴College of Forestry, Northwest A&F University, Yangling 712100, Shaanxi, China

Received:2019-12-30 Revised:2020-05-25 Online:2020-08-15 Published:2021-02-15
Supported by:
This work was supported by the National Key R&D Program of China (2016YFC0501707) and the National Natural Science Foundation of China (41771557).

摘要/Abstract

摘要： 土壤酶是有机质降解的催化剂,其动力学特征是表征酶催化性能的重要指标,对评价土壤健康质量有重要作用。本研究选择黄土高原3种植被带下人工刺槐林土壤为对象,探讨了土壤酶动力学参数对温度变化的响应及其温度敏感性(Q₁₀)的变化特征。结果表明: 随着培养温度的升高,土壤丙氨酸转氨酶、亮氨酸氨基肽酶和碱性磷酸酶的潜在最大反应速率(V_max)和半饱和常数(K_m)均呈线性增加,且V_max呈现出森林带>森林草原带>草原带的地带性规律。V_max的温度敏感性(Q10(V_max))为1.14～1.62,K_m的温度敏感性(Q_{10(K_m)})为1.05～1.47,且两者在森林草原带的值均低于其他植被带。在低、高温区,不同土壤酶的Q₁₀在各植被带间的变化也不尽相同。冗余分析显示,Q₁₀与环境变量尤其是土壤养分有显著的相关关系,这表明Q₁₀可能还受到除温度以外其他环境因子的影响。

关键词: 黄土高原, 植被带, 土壤酶动力学参数, 温度敏感性

Abstract: Soil enzymes are catalysts for organic matter decomposition, the kinetic characteristics of which are important indicators of the catalytic performance of enzymes, with important role in evalua-ting soil health quality. We examined the responses of soil enzyme kinetic parameters to temperature change and the variation characteristics of their temperature sensitivity (Q₁₀) in Robinia pseu-doacacia plantation soil under three different vegetation zones on the Loess Plateau. The results showed that the potential maximum reaction rate (V_max) and the half-saturation constant (K_m) of alanine transaminase (ALT), leucine aminopeptidase (LAP) and alkaline phosphatase (ALP) all increased linearly with the increasing incubation temperature. The zonal regularity of forest zone > forest-steppe zone > steppe zone was presented in V_max. The temperature sensitivity of V_max(Q10(V_max)) ranged from 1.14 to 1.62, and the temperature sensitivity of K_m(Q_{10(K_m)}) ranged from 1.05 to 1.47, with both values being lower in forest-steppe zone than other vegetation zones. In low and high temperature regions, the variations of Q₁₀ in different soil enzymes differed among vegetation zones. Results from redundancy analysis showed that Q₁₀ had a significant correlation with environmental variables, especially soil nutrients, indicating that Q₁₀ would be affected by other environmental factors besides temperature.

Key words: Loess Plateau, vegetation zone, soil enzyme kinetic parameter, temperature sensitivity

赵子文, 王国梁, 吴阳, 陈文静, 李秧秧, 刘国彬, 薛萐. 黄土高原不同植被带人工刺槐林土壤氮磷转化酶动力学参数及其温度敏感性[J]. 应用生态学报, 2020, 31(8): 2515-2522.

ZHAO Zi-wen, WANG Guo-liang, WU Yang, CHEN Wen-jing, LI Yang-yang, LIU Guo-bin, XUE Sha. Kinetic parameters and temperature sensitivity of soil nitrogen and phosphorus transforming enzymes in Robinia pseudoacacia plantations under different vegetation zones on the Loess Plateau, China[J]. Chinese Journal of Applied Ecology, 2020, 31(8): 2515-2522.

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黄土高原不同植被带人工刺槐林土壤氮磷转化酶动力学参数及其温度敏感性

Kinetic parameters and temperature sensitivity of soil nitrogen and phosphorus transforming enzymes in Robinia pseudoacacia plantations under different vegetation zones on the Loess Plateau, China

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