碳氮有效性对亚热带人工林土壤微生物呼吸及其代谢响应的影响

doi:10.13287/j.1001-9332.202408.008

摘要/Abstract

摘要： 本研究以福建三明森林生态系统国家野外观测研究站的青冈、格氏栲、马尾松、闽楠、香樟林5种人工林为研究对象,分析了0～10 cm表层土壤微生物呼吸对葡萄糖添加的代谢响应。结果表明:与对照相比,葡萄糖添加使土壤微生物呼吸提高了82.4%～349.5%,不同树种间差异显著。对照中,土壤微生物呼吸与微生物生物量、土壤有机碳及真菌/细菌有显著的相关关系,表明在没有活性碳供应时,微生物代谢受土壤有机碳含量的调控,且与土壤微生物生物量和群落结构有关。在葡萄糖添加处理中,土壤微生物呼吸与土壤总氮、溶解性有机氮和矿质氮呈显著正相关,表明当活性碳供应充足时,微生物代谢主要受土壤氮含量及其有效性制约。微生物呼吸的代谢响应,即葡萄糖添加处理与对照土壤微生物呼吸的比值,主要受土壤碳氮比的影响,呈现出微生物代谢响应随土壤碳氮比的下降而升高。此外,土壤pH也是影响微生物代谢响应的重要因素。土壤碳、氮含量及有效性对微生物呼吸的影响依赖于微生物是否受碳限制,土壤碳含量调控了碳限制时的微生物呼吸,而土壤氮含量及有效性调控了碳限制解除后的微生物呼吸。

关键词: 碳限制, 化学计量学, 外源活性碳输入, 微生物群落结构, 异养呼吸

Abstract: We examined the metabolic response of microbial respiration to glucose addition with the topsoil (0-10 cm) from five plantation types, including Quercus glauca, Castanopsis kawakamii, Pinus massoniana, Phoebe bournei, and Cinnamomum camphora plantations, in the Sanming Forest Ecosystem National Field Observation and Research Station in Fujian Province. The results showed that glucose addition significantly increased microbial respiration by 82.4%-349.5%, with significant difference among tree species. In the control, microbial respiration significantly correlated with microbial biomass carbon, soil organic carbon, and the fungi/bacteria ratio, indicating that microbial metabolism was regulated by soil organic carbon content and was associated with microbial biomass and community structure in the absence of labile carbon supply. In the glucose addition treatment, microbial respiration positively correlated with soil total nitrogen, dissolved organic nitrogen, and mineral nitrogen, indicating that microbial metabolism was mainly constrained by soil nitrogen content and its availability in the presence of adequate labile carbon supply. The metabolic response of microbial respiration, as indicated by the ratio of microbial respiration in the glucose addition treatment to that in the control, was primarily affected by soil carbon/nitrogen ratio, with a decrease in the ratio leading to an increase in the microbial metabolic response. Additionally, soil pH played an important role in mediating microbial metabolic response. The effect of the content and availability of soil carbon and nitrogen on microbial respiration depended on whether microbes were carbon-limited. Soil carbon content media-ted microbial respiration when microbes were carbon-limited, whereas soil nitrogen content and availability mediated microbial respiration after the alleviation of microbial carbon limitation.

Key words: carbon limitation, stoichiometry, exogenous labile C input, microbial community structure, heterotrophic respiration

高红, 王小红, 吴东梅, 范爱连, 贾林巧, 姚晓东, 陈光水. 碳氮有效性对亚热带人工林土壤微生物呼吸及其代谢响应的影响[J]. 应用生态学报, 2024, 35(8): 2025-2034.

GAO Hong, WANG Xiaohong, WU Dongmei, FAN Ailian, JIA Linqiao, YAO Xiaodong, CHEN Guangshui. Effects of carbon and nitrogen availability on soil microbial respiration and its metabolic response in subtropical plantations[J]. Chinese Journal of Applied Ecology, 2024, 35(8): 2025-2034.

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