酶化学计量揭示5年氮添加加剧毛竹林土壤微生物碳磷限制

doi:10.13287/j.1001-9332.202102.038

应用生态学报 ›› 2021, Vol. 32 ›› Issue (2): 521-528.doi: 10.13287/j.1001-9332.202102.038

酶化学计量揭示5年氮添加加剧毛竹林土壤微生物碳磷限制

曾泉鑫^1,2, 张秋芳^1,2, 林开淼^2,3, 周嘉聪^1,2, 元晓春^1,2, 梅孔灿^1,2, 吴玥^1,2, 崔琚琰^1,2, 徐建国^2,3, 陈岳民^1,2*

¹福建师范大学地理科学学院, 福州 350007;
²湿润亚热带山地生态国家重点实验室培育基地, 福州 350007;
³福建戴云山国家级自然保护区管理局, 福建泉州 362500

收稿日期:2020-06-22 接受日期:2020-11-04 出版日期:2021-02-15 发布日期:2021-08-15
通讯作者: ^*E-mail: ymchen@fjnu.edu.cn
作者简介:曾泉鑫, 男, 1995年生, 硕士研究生。主要从事全球变化与森林土壤研究。E-mail: 645823769@qq.com
基金资助:
国家自然科学基金项目(31670620)和福建省自然科学基金项目(2019J05163)资助

Enzyme stoichiometry evidence revealed that five years nitrogen addition exacerbated the carbon and phosphorus limitation of soil microorganisms in a Phyllostachys pubescens forest

ZENG Quan-xin^1,2, ZHANG Qiu-fang^1,2, LIN Kai-miao^2,3, ZHOU Jia-cong^1,2, YUAN Xiao-cun^1,2, MEI Kong-can^1,2, WU Yue^1,2, CUI Ju-yan^1,2, XU Jian-guo^2,3, CHEN Yueh-min^1,2*

¹School of Geographical Science, Fujian Normal University, Fuzhou 350007, China;
²Cultivation Base of State Key Laboratory of Humid Subtropical Mountain Ecology, Fuzhou 350007, China;
³Daiyun Mountain National Nature Reserve Administration Bureau, Quanzhou 362500, Fujian, China

Received:2020-06-22 Accepted:2020-11-04 Online:2021-02-15 Published:2021-08-15
Contact: ^*E-mail: ymchen@fjnu.edu.cn
Supported by:
National Natural Science Foundation of China (31670620) and the Natural Science Foundation of Fujian (2019J05163)

摘要/Abstract

摘要： 土壤胞外酶活性和酶化学计量比能很好地反映土壤养分有效性和微生物对养分的需求变化。然而,氮(N)沉降对亚热带森林土壤微生物养分相对限制情况的影响尚不清楚。通过在亚热带毛竹林进行N添加试验来模拟N沉降,并在试验满5年时进行取样,测定不同处理下土壤养分和与碳(C)、N、磷(P)循环相关的酶活性,利用酶化学计量比及矢量分析探究微生物的养分分配情况。结果表明: N添加显著降低土壤可溶性有机碳、有效磷含量,显著提高有效氮含量。此外,N添加显著降低β-N-乙酰氨基葡糖苷酶(NAG)活性和NAG/微生物生物量碳(MBC),显著提高酸性磷酸酶(ACP)和ACP/MBC。低N和中N处理显著提高酶C/N、矢量长度和矢量角度,但显著降低酶N/P。冗余分析表明,N添加下,土壤有效磷含量的变化是影响土壤酶活性及酶化学计量比变化的主要因子。综上可知,N添加改变了微生物的养分获取策略,即通过减少分配给合成N获取酶的养分来增加合成P获取酶的养分。此外,N添加还加剧了微生物的C、P限制,未来可以施加适量P肥来提高亚热带毛竹林的土壤肥力。

关键词: 氮添加, 酶活性, 酶化学计量, 养分限制

Abstract: The activity and stoichiometry of soil extracellular enzyme can provide a good indication for changes in soil nutrient availability and microbial demands for nutrients. However, it remains unclear how would nitrogen (N) deposition affect nutrient limitation of microbes in subtropical forest soils. We conducted a 5 years N addition experiment in a subtropical Phyllostachys pubescens forest. The soil nutrients and enzyme activities associated with carbon (C), N, and phosphorus (P) cycles were measured. We also examined the nutrient distribution of microorganisms using enzyme stoichiometry and vector analysis. The results showed that N addition significantly decreased the contents of soil soluble organic C and available P and increased that of available N. Furthermore, N addition significantly decreased β-N-acetyl-glucosaminidase (NAG) activity and NAG/ microbial biomass carbon (MBC), and increased acid phosphatase (ACP) and ACP/MBC. The low and moderate N addition levels significantly increased enzyme C/P, vector length, and vector angle, but significantly decreased enzyme N/P. Results of redundancy analysis showed that the change in soil enzyme activity and enzymatic stoichiometry were mainly driven by soil available P content under N addition. In summary, N addition altered the microbial nutrient acquisition strategy, which increased nutrient allocation to P-acquiring enzyme production but reduced that to N-acquiring enzyme production. Moreover, N addition exacerbated the C and P limitation of soil microorganisms. Appropriate amount of P fertilizer could be applied to improve soil fertility of subtropical P. pubescens forest in the future.

Key words: nitrogen addition, enzyme activity, enzyme stoichiometry, nutrient restriction

曾泉鑫, 张秋芳, 林开淼, 周嘉聪, 元晓春, 梅孔灿, 吴玥, 崔琚琰, 徐建国, 陈岳民. 酶化学计量揭示5年氮添加加剧毛竹林土壤微生物碳磷限制[J]. 应用生态学报, 2021, 32(2): 521-528.

ZENG Quan-xin, ZHANG Qiu-fang, LIN Kai-miao, ZHOU Jia-cong, YUAN Xiao-cun, MEI Kong-can, WU Yue, CUI Ju-yan, XU Jian-guo, CHEN Yueh-min. Enzyme stoichiometry evidence revealed that five years nitrogen addition exacerbated the carbon and phosphorus limitation of soil microorganisms in a Phyllostachys pubescens forest[J]. Chinese Journal of Applied Ecology, 2021, 32(2): 521-528.

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酶化学计量揭示5年氮添加加剧毛竹林土壤微生物碳磷限制

Enzyme stoichiometry evidence revealed that five years nitrogen addition exacerbated the carbon and phosphorus limitation of soil microorganisms in a Phyllostachys pubescens forest

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