温度和初始pH对生物结皮中4种优势蓝藻生长的影响

doi:10.13287/j.1001-9332.202402.028

应用生态学报 ›› 2024, Vol. 35 ›› Issue (2): 516-522.doi: 10.13287/j.1001-9332.202402.028

温度和初始pH对生物结皮中4种优势蓝藻生长的影响

王一帆¹, 李璇¹, 罗珊珊², 黄卓超¹, 丁真玉珍¹, 周楠¹, 赵允格^3,4*

¹西北农林科技大学资源环境学院, 陕西杨凌 712100;
²西北农林科技大学生命科学学院, 陕西杨凌 712100;
³西北农林科技大学水土保持科学与工程学院, 陕西杨凌 712100;
⁴西北农林科技大学水土保持研究所, 黄土高原土壤侵蚀与旱地农业国家重点实验室, 陕西杨凌 712100

收稿日期:2023-06-14 修回日期:2023-10-07 出版日期:2024-02-18 发布日期:2024-08-18
通讯作者: ^*E-mail: zyunge@ms.iswc.ac.cn
作者简介:王一帆, 女, 2002年生, 本科生。主要从事资源环境研究。E-mail: 1756637518@qq.com
基金资助:
国家重点研发计划课题(2022YFF1300802)、国家自然科学基金项目(41830758)和中央高校基本科研业务费专项资金(2452020237)

Effects of temperature and initial pH on the growth of four dominant cyanobacteria species in biological soil crusts

WANG Yifan¹, LI Xuan¹, LUO Shanshan², HUANG Zhuochao¹, Dingzhenyuzhen¹, ZHOU Nan¹, ZHAO Yunge^3,4*

¹College of Resources and Environment, Northwest A＆F University, Yangling 712100, Shaanxi, China;
²College of Life Sciences, Northwest A＆F University, Yangling 712100, Shaanxi, China;
³College of Soil and Water Conservation Science and Engineering, Northwest A＆F University, Yangling 712100, Shaanxi, China;
⁴State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Institute of Soil and Water Conservation, Northwest A＆F University, Yangling 712100, Shaanxi, China

Received:2023-06-14 Revised:2023-10-07 Online:2024-02-18 Published:2024-08-18

摘要/Abstract

摘要： 生物土壤结皮对干旱半干旱地区生态环境健康及可持续发展有重要意义。具鞘微鞘藻、伪枝藻、念珠藻和鱼腥藻是生物结皮中蓝藻的优势种,对其大规模培养有着广阔的应用前景。以4种蓝藻为材料,采用室内液体培养方法,研究了5个温度(10、20、25、30、35 ℃)和5个溶液初始pH(4、6、8、10、12)对4种藻生物量和溶液pH的影响。结果表明: 在20 ℃以下温度培养,供试4种藻生物量增长缓慢,在25～35 ℃下4种藻均能正常生长,其中25和30 ℃下藻类生物量增长最快。不同藻种的最适培养温度略有区别,伪枝藻和念珠藻最适培养温度为25～30 ℃,具鞘微鞘藻和鱼腥藻为30 ℃。供试4种藻对溶液pH有极强的调节能力,均能够在5个初始pH环境中进行增殖,在初始pH为4的培养环境下能达到最大生物量和比生长速率,在初始pH为12下生物量和比生长速率最低。研究为生物结皮中优势藻的扩繁提供了科学依据。

关键词: 具鞘微鞘藻, 伪枝藻, 念珠藻, 鱼腥藻, 生物量, pH

Abstract: Biological soil crusts are of great significance for environment health and sustainable development in arid and semi-arid areas. Cyanobacteria, Microcoleus vaginatus, Scytonema sp., Nostoc sp., and Anabaena sp. are the dominant species in microbial community of biological soil crusts worldwide. Considering their broad application prospect, it is meaningful to cultivate them extensively. We examined the effects of temperature (10, 20, 25, 30, 35 ℃) and initial pH (4, 6, 8, 10, 12) on biomass and solution pH towards the four species of cyanobacteria with liquid culture in laboratory. The results showed that the biomass of the four cyanobacterial species grew slowly under 20 ℃, and that all species could grow in 25-35 ℃, with the highest growth rate at 25 and 30 ℃. The optimum culture temperature of different cyanobacterial species was slightly different. The optimum culture temperature was 25-30 ℃ for Scytonema sp. and Nostoc sp., and 30 ℃ for M. vaginatus and Anabaena sp. The four cyanobacterial species had a strong ability to adjust solution pH and proliferate in five different initial pH conditions. The highest maximum biomass and specific growth rate were recorded in the culture environment with initial pH of 4, while the lowest maximum biomass and specific growth rate were observed in initial pH of 12. Our results would provide scientific basis for the propagation of dominant cyanobacteria in biological soil crusts.

Key words: Microcoleus vaginatus, Scytonema sp., Nostoc sp., Anabaena sp., biomass, pH

王一帆, 李璇, 罗珊珊, 黄卓超, 丁真玉珍, 周楠, 赵允格. 温度和初始pH对生物结皮中4种优势蓝藻生长的影响[J]. 应用生态学报, 2024, 35(2): 516-522.

WANG Yifan, LI Xuan, LUO Shanshan, HUANG Zhuochao, Dingzhenyuzhen, ZHOU Nan, ZHAO Yunge. Effects of temperature and initial pH on the growth of four dominant cyanobacteria species in biological soil crusts[J]. Chinese Journal of Applied Ecology, 2024, 35(2): 516-522.

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温度和初始pH对生物结皮中4种优势蓝藻生长的影响

Effects of temperature and initial pH on the growth of four dominant cyanobacteria species in biological soil crusts

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