工程受损区耐寒溶磷菌的筛选、鉴定及促生效应

doi:10.13287/j.1001-9332.202604.038

应用生态学报 ›› 2026, Vol. 37 ›› Issue (4): 1247-1256.doi: 10.13287/j.1001-9332.202604.038

工程受损区耐寒溶磷菌的筛选、鉴定及促生效应

杨贵巧^1,2,3, 詹娟^1,3, 许维宏⁴, 刘庆华^1,3, 汪鹏鹏^1,3, 张胜², 王健美², 庞学勇^1,3*

¹中国科学院成都生物研究所, 山地生态恢复与生物多样性保护四川省重点实验室, 成都 610213;
²四川大学生命科学学院, 成都 610059;
³中国科学院茂县山地生态系统定位研究站, 四川茂县 623299;
⁴茂县林业和草原局, 四川茂县 623299

收稿日期:2025-11-28 修回日期:2026-03-17 出版日期:2026-04-18 发布日期:2026-05-29
通讯作者: *E-mail: pangxy@cib.ac.cn
作者简介:杨贵巧, 女, 2000年生, 硕士研究生。主要从事土壤微生物研究。E-mail: 2023222045183@stu.scu.edu.cn
基金资助:
国家自然科学基金项目(32572029,32171756)、四川省中央引导地方科技发展专项(2025ZYD0007)、西藏自治区科技计划技术创新引导项目(XZ202501JX0012)和中国电力建设集团有限公司藏区水电开发工程项目(DJ-ZDXM-2024-28)

Screening, identification, and growth-promoting effects of cold-tolerant phosphate-solubilizing bacteria from high-altitude cold engineering area

YANG Guiqiao^1,2,3, ZHAN Juan^1,3, XU Weihong⁴, LIU Qinghua^1,3, WANG Pengpeng^1,3, ZHANG Sheng², WANG Jianmei², PANG Xueyong^1,3*

¹Mountain Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610213, China;
²School of Life Sciences, Sichuan University, Chengdu 610059, China;
³Mao-xian Mountain Ecosystem Research Station, Chinese Academy of Sciences, Maoxian 623299, Sichuan, China;
⁴Mao-xian Bureau of Forestry and Grassland, Maoxian 623299, Sichuan, China

Received:2025-11-28 Revised:2026-03-17 Online:2026-04-18 Published:2026-05-29

摘要/Abstract

摘要： 高寒工程受损区生态恢复因土壤磷素匮乏与低温环境而面临严峻挑战。化学磷肥效率低下且易造成环境风险,而常规溶磷菌在低温下活性受限。因此,筛选兼具耐寒特性与高效溶磷促生功能的微生物资源,对于实现高寒地区的绿色生态修复至关重要。本研究从川西高原工程扰动区土壤中分离筛选耐寒溶磷菌,通过多温度梯度(6、10、28 ℃)评估其溶磷能力以及分泌有机酸、铁载体和吲哚乙酸(IAA)的能力,并通过盆栽试验验证其促生效应。共获得3株高效耐寒溶磷菌(P-6、P-18与P-25),鉴定为假单胞菌属。它们在6~28 ℃范围内均保持良好的溶磷能力,其中P-25在10 ℃时溶磷量最高(481.26 mg·L^-1)。机理分析揭示,3株菌株主要通过分泌有机酸与铁载体活化无机磷,P-18与P-25还能合成IAA。盆栽试验表明,接种菌株可显著提高土壤有效磷含量,促进垂穗披碱草生长,其中P-25处理效果最佳,植株磷含量、生物量及株高分别较对照提升109.2%、65.2%和6.7%。3株耐寒溶磷菌兼具高效溶磷与促生功能,在高寒工程迹地生态修复中具有应用潜力。

关键词: 耐寒溶磷菌, 生态恢复, 促生作用, 溶磷机制

Abstract: Ecological restoration of degraded alpine engineering areas faced severe challenges due to soil phospho-rus deficiency and low-temperature. Chemical phosphorus fertilizers had low efficiency and easily caused environmental risks, while conventional phosphate-solubilizing bacteria showed lower activity at low temperatures. Therefore, screening microbial resources with both cold tolerance and high-efficiency phosphate-solubilizing and growth-promoting functions was crucial for achieving green ecological restoration in alpine regions. In this study, we isolated and screened cold-tolerant phosphate-solubilizing bacteria from soils of disturbed engineering areas on the Wes-tern Sichuan Plateau. We evaluated their phosphate-solubilizing capacity and abilities to secrete organic acids, si-derophores, and indole-3-acetic acid (IAA) across a temperature gradient (6, 10, and 28 ℃), and verified their growth-promoting effects in a pot experiment. We obtained three highly efficient cold-tolerant phosphate-solubilizing strains (P-6, P-18, and P-25), which were identified as members of Pseudomonas genus. These strains maintained good phosphate-solubilizing capacity within the range of 6-28 ℃, among which strain P-25 exhibited the highest phosphorus solubilization (481.26 mg·L^-1) at 10 ℃. Mechanistic analysis revealed that the three strains activated inorganic phosphorus mainly by secreting organic acids and siderophores, and strains P-18 and P-25 could synthesize IAA. Inoculation with these strains significantly increased soil available phosphorus content and promoted the growth of Elymus nutans. The P-25 treatment performed the best, with plant phosphorus content, biomass, and plant height being increased by 109.2%, 65.2%, and 6.7%, respectively. All the three cold-tolerant phosphate-solubilizing bacteria strains possessed both high-efficiency phosphate-solubilizing and growth-promoting functions, and thus had application potential in the ecological restoration of alpine engineering sites.

Key words: cold-tolerant phosphate-solubilizing bacteria, ecological restoration, growth promotion, phosphate-solubilizing mechanism

杨贵巧, 詹娟, 许维宏, 刘庆华, 汪鹏鹏, 张胜, 王健美, 庞学勇. 工程受损区耐寒溶磷菌的筛选、鉴定及促生效应[J]. 应用生态学报, 2026, 37(4): 1247-1256.

YANG Guiqiao, ZHAN Juan, XU Weihong, LIU Qinghua, WANG Pengpeng, ZHANG Sheng, WANG Jianmei, PANG Xueyong. Screening, identification, and growth-promoting effects of cold-tolerant phosphate-solubilizing bacteria from high-altitude cold engineering area[J]. Chinese Journal of Applied Ecology, 2026, 37(4): 1247-1256.

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工程受损区耐寒溶磷菌的筛选、鉴定及促生效应

Screening, identification, and growth-promoting effects of cold-tolerant phosphate-solubilizing bacteria from high-altitude cold engineering area

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