Investigation on the resistance of wheat germplasm resources to aphid based on fuzzy recognition and electrical penetration graph (EPG)techniques

doi:10.13287/j.1001-9332.202010.033

Abstract

Abstract: We analyzed the resistance of wheat germplasm resources with different genetic backgrounds to aphids by fuzzy recognition technique in field and electrical penetration graph (EPG) in laboratory, with the aim to find new aphid-resistance resource. Results from a two-year field experiment showed that most trititrigia had medium and low resistance, while most triticale were medium and low susceptible. The trititrigia 21 (medium resistance), trititrigia 22 (low resistance), triticale 31 (medium susceptable) and triticale 32 (low susceptable) with stable resistance traits and diffe-rent resistance levels were selected for feeding behavior analysis of Sitobion avenae. We analyzed the basic waveforms, including non-feeding wave (Np), probing wave (P), potential drop (Pd), secretion of water saliva wave (E1), phloem feeding wave (E2), cellular mechanical hindrance wave (F), and xylem feeding wave (G). The time for initial probe of S. avenae on trititrigia was significantly longer than that of triticale, while the duration of E1 wave on trititrigia was significantly longer than that of triticale. The duration of F wave on trititrigia 21 and the Np wave on the trititrigia 22 were the longest, while that of P wave on triticale 31 and the G wave on triticale 32 were the longest. Taking the durations of E1, F and Np waves as indices, the field identification on the resis-tance of wheat germplasm resources to aphid based on fuzzy recognition technique was basically consistent with that using EPG. Our results suggest that E1, F and Np waves could be used to evaluate the resistance levels of wheat to aphid. Tititrigia 21 and 22 were more resistant to aphid, which could be used as the germplasm for breeding aphid-resistance wheat varieties.

Key words: germplasm resouce, wheat, fuzzy recognition technique, electrical penetration graph, plant resistance to aphid

JIANG Ya-xiu, CHANG Wen-jing, ZHAN Yi-di, LIU Zhen, LIU Yong. Investigation on the resistance of wheat germplasm resources to aphid based on fuzzy recognition and electrical penetration graph (EPG)techniques[J]. Chinese Journal of Applied Ecology, 2020, 31(10): 3248-3254.

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