微生物菌剂对猪粪堆肥中细菌群落结构的影响

doi:10.13287/j.1001-9332.202007.033

应用生态学报 ›› 2020, Vol. 31 ›› Issue (7): 2449-2456.doi: 10.13287/j.1001-9332.202007.033

微生物菌剂对猪粪堆肥中细菌群落结构的影响

王建才^1,2,3, 朱荣生^1,2,3, 刘兴华^1,2,3, 孙守礼^1,2,3, 王怀中^1,2,3, 唐茜^1,2,3, 齐波⁴, 黄保华^1,2,3*

¹山东省农业科学院畜牧兽医研究所, 济南 250100;
²山东省畜禽疫病防治与繁育重点实验室, 济南 250100;
³山东省畜禽健康养殖工程中心, 济南 250100;
⁴济南海华生物科技有限公司, 济南 250108

收稿日期:2019-10-21 接受日期:2020-04-03 出版日期:2020-07-15 发布日期:2021-01-15
通讯作者: E-mail: jn-hbh@163.com
作者简介:王建才, 男, 1985年生, 博士。主要从事耐重金属菌株筛选和重金属污染防治研究。E-mail: wangjiancai66@126.com
基金资助:
中国博士后科学基金项目(2017M612316)、山东省农业科学院农业科技创新工程项目(CXGC2016B13)和山东省重点研发计划项目(2017CXGC0309)资助

Effects of microbial agents on bacterial community composition during swine manure composting

WANG Jian-cai^1,2,3, ZHU Rong-sheng^1,2,3, LIU Xing-hua^1,2,3, SUN Shou-li^1,2,3, WANG Huai-zhong^1,2,3, TANG Qian^1,2,3, QI Bo⁴, HUANG Bao-hua^1,2,3*

¹Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Ji'nan 250100, China;
²Shandong Provincial Key Laboratory of Animal Disease Control and Breeding, Ji'nan 250100, China;
³Shandong Provincial Engineering Technology Center of Animal Healthy Breeding, Ji'nan 250100, China;
⁴Jinan Haihua Biotechnology Co., Ltd., Ji'nan 250108, China

Received:2019-10-21 Accepted:2020-04-03 Online:2020-07-15 Published:2021-01-15
Contact: E-mail: jn-hbh@163.com
Supported by:
This work was supported by the China Postdoctoral Science Foundation (2017M612316), the Agricultural Scientific and Technological Innovation Project of Shandong Academy of Agricultural Sciences (CXGC2016B13) and the Key R&D Project of Shandong Province (2017CXGC0309).

摘要/Abstract

摘要： 以猪粪和小麦秸秆做堆肥试验,处理组添加外源微生物菌剂,利用常规方法对堆肥样品进行理化性状测定,采用高通量测序技术分析堆肥过程中细菌群落特征。理化性状测定结果表明: 添加外源菌剂可延长堆肥高温时间,降低堆肥发酵末期的pH,增加全氮含量,加快C/N的下降。主成分分析表明: 外源菌剂影响堆肥样品细菌群落的稳定性。门分类水平上,厚壁菌门、变形菌门和绿弯菌门的相对丰度在处理组中较高;纲分类水平上,梭状芽孢杆菌纲、α-变形菌纲和γ-变形菌纲在处理组的升温期和高温期相对丰度增加;科分类水平上,小单孢菌科和梭状芽孢杆菌纲的消化链球菌科、梭菌科以及盐厌氧菌科的相对丰度在处理组的升温期和高温期均呈上升趋势。Pearson相关性分析表明,盐胞菌属与外源菌剂呈显著正相关,而氨苄芽孢杆菌属与外源菌剂呈显著负相关。研究表明,猪粪堆肥中添加外源菌剂可使堆肥的理化性质和细菌群落结构均发生显著变化。

关键词: 猪粪, 高通量测序, 外源菌剂, 理化参数, 微生物群落, Pearson相关性热图

Abstract: The process of swine manure and wheat straw aerobic composting was examined, with exogenous microbial agents being added in treatment group. The physicochemical properties were measured by conventional methods, and bacterial community characteristics were investigated by high throughput sequencing analysis. Exogenous microbial agents increased high-temperature duration, reduced pH value at the end of fermentation stage, augmented total nitrogen content, reduced C/N ratio. Results from principal component analysis showed that microbial agents affected the stability of bacterial community during composting. At the phylum level, the relative abundance of Firmicutes, Proteobacteria, and Chloroflexi was higher in the treatment group. At the class level, the relative abundance of Clostridia, Alphaproteobacteria, and Gammaproteobacteria in the treatment group were higher at the mesophilic and thermophilic phases. At the family level, Peptostreptococcaceae, Clostridiaceae_1, and Halanaerobiaceae of the Clostridia and Micromonosporaceae in the treatment group were higher at the mesophilic and thermophilic phases. Halocella was significantly positively correlated with exogenous microbial agents, while Ammoniibacillus was significantly negatively correlated with it. It suggested that microbial agents significantly changed the physicochemical properties and bacterial community structure during swine composting.

Key words: swine manure, high throughput sequencing, exogenous microbial agent, physiochemi-cal parameter, microbial community, Pearson correlation heatmap

王建才, 朱荣生, 刘兴华, 孙守礼, 王怀中, 唐茜, 齐波, 黄保华. 微生物菌剂对猪粪堆肥中细菌群落结构的影响[J]. 应用生态学报, 2020, 31(7): 2449-2456.

WANG Jian-cai, ZHU Rong-sheng, LIU Xing-hua, SUN Shou-li, WANG Huai-zhong, TANG Qian, QI Bo, HUANG Bao-hua. Effects of microbial agents on bacterial community composition during swine manure composting[J]. Chinese Journal of Applied Ecology, 2020, 31(7): 2449-2456.

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微生物菌剂对猪粪堆肥中细菌群落结构的影响

Effects of microbial agents on bacterial community composition during swine manure composting

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