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应用生态学报 ›› 1999, Vol. 10 ›› Issue (6): 729-731.

• 研究论文 • 上一篇    下一篇

环境温度条件对口蘑菌L1纤维素酶和木聚糖酶合成的影响

蔡敬民1, 吴克1, 张洁1, 刘云峰1, 潘仁瑞1, 丁佐龙2   

  1. 1. 合肥联合大学生化系, 合肥230022;
    2. 安徽农业大学林产工业研究所, 合肥230036
  • 收稿日期:1998-02-25 修回日期:1999-03-04 出版日期:1999-11-25 发布日期:1999-11-25
  • 通讯作者: 蔡敬民,男,1963年生,副教授,微生物学专业方向,在国内外学术刊物上发表论文28篇.
  • 基金资助:

    安徽省自然科学基金(98241515)和安徽省教委科研基金资助项目(96JL0155).

Effect of environmental temperature on formation of cellulase and xylanase in Thricholomataceae L1

Cai Jingmin1, Wu Ke1, Zhang Jie1, Liu Yunfeng1, Pan Renrui1, Ding Zuolong2   

  1. 1. Hefei Union University, Hefei 230022;
    2. Anhui Agricultural University, Hefei 230036
  • Received:1998-02-25 Revised:1999-03-04 Online:1999-11-25 Published:1999-11-25

摘要: 较高的环境温度有利于口蘑菌L1的生长和蛋白质的合成。当环境温度为38℃时,纤维素酶的比活力达最高值,为23-4Iu·mg-1蛋白质,该菌在此温度下培养90h,纤维素酶活力为33Iu·g-1干培养基。木聚糖酶的最适合成温度为32℃,此温度下木聚糖酶的比活力可达92Iu·mg-1蛋白质,培养67h,木聚糖酶活力可达126Iu·g-1干培养基。提示环境温度对口蘑菌L1细胞内酶的合成具有调节作用。

关键词: 温度, 口蘑菌, 纤维素酶, 木聚糖酶

Abstract: Higher environmental temperature is beneficial to the growth of Tricholomataceae L1 and its protein synthesis. When temperature is 38℃, the specific activity of cellulase is the highest, reaching 23.4Iu·mg-1 protein. After cultivated for 93 hours at this temperature, its cellulase activity is 33Iu·g-1 dry medium. The optimal temperature for the formation of xylanase is 32℃. Under this temperatrue, the specific activity of xylanase is 92Iu·mg-1 protein, and after cultivated for 67 hours, it is 126Iu·g-1 dry medium. It is suggested that environmental temperature has a regulation effect on the formation of intracellular enzyme of Tricholomataceae L1.

Key words: Temperature, Tricholomataceae, Cellulase, Xylanase