欢迎访问《应用生态学报》官方网站,今天是 分享到:

应用生态学报 ›› 1999, Vol. 10 ›› Issue (6): 735-738.

• 研究论文 • 上一篇    下一篇

水稻体内细菌的动态研究

刘云霞1, 张青文2, 周明牂2   

  1. 1. 中国农业科学院生物防治研究所, 北京100081;
    2. 中国农业大学植保系, 北京100094
  • 收稿日期:1997-11-10 修回日期:1998-03-16 出版日期:1999-11-25 发布日期:1999-11-25
  • 通讯作者: 刘云霞,女,33岁,博士,助研.主要从事植物微生物昆虫的互作关系及昆虫病原菌的研究,发表相关论文6篇.
  • 基金资助:

    瑞士青年科学基金(IFS:AC/11134)和农业部农作物病虫草害生物防治资源研究与利用重点开放实验室(LOBCR-96-04)资助项目.

Population dynamics of endophytic bacteria in symptom free rice plants

Liu Yunxia1, Zhang Qingwen2, Zhuo Mingzang2   

  1. 1. Institute of Biological Control, Chinese Academy of Agricultural Sciences, Beijing 100081;
    2. Department of Plant Protection, China Agricultural University, Beijing 100094
  • Received:1997-11-10 Revised:1998-03-16 Online:1999-11-25 Published:1999-11-25

摘要: 经过对水稻两品种(沈农319、中百4号)不同时期、不同组织内生细菌动态变化研究结果表明,根组织带菌量最高,其次是叶,茎最低。发育阶段以孕穗期带菌量显着增高,随着组织衰老而降低。对分离到的4个主要种群显着性检验结果表明,巨大芽孢杆菌为两品种体内细菌优势种。通过对水稻这一世界性粮食作物体内细菌的种类,以及随生育期、组织间菌体数量变化的探讨研究,为水稻害虫的生物防治,提供遗传改良工程杀虫细菌的有效载体菌。

关键词: 水稻, 体内细菌, 巨大芽孢杆菌, 生防

Abstract: The population dynamics of endophytic bacteria in different tissues of rice plants (cultivars Shennong 319 and Zhongbai 4) was studied during their development stages. The results showed that the population of endophytic bacteria was the largest in roots, followed by in leaves and stems. And the population size was the largest at tillering stage, and gradually decreased with the senescence of tissues. Among the four isolated bacterial species, Bacillus megaterium was the dominant endophytic bacterium in the two rice cultivars. The studies on the species of endophytic bacteria and their population dynamics in tissues with the growth of rice plants could provide effective carrier bacteria to select the insect killing bacteria in genetic improvement engineering for biological control of rice insects.

Key words: Rice, Endophytic bacteria, Bacillus megaterium, Biological control