阿维菌素在土壤中的微生物降解研究

应用生态学报 ›› 2004, Vol. ›› Issue (11): 2175-2178.

阿维菌素在土壤中的微生物降解研究

张卫¹, 虞云龙², 林匡飞¹, 李少南², 吴加伦², 樊德方²

1. 华东理工大学资环学院, 上海 200237;
2. 浙江大学农药环境毒理研究所, 杭州 310029

收稿日期:2002-10-12 修回日期:2003-02-28
通讯作者: 张卫,男,1975年生,博士,主要从事环境安全性评价、生物修复和生态毒理学方面的研究,发表论文10余篇.E-mail:zwzju@sohu.com
基金资助:
国家自然科学基金资助项目(30270880)

Microbial degradation of Abamectin in soil

ZHANG Wei¹, YU Yunlong², LIN Kuangfei¹, LI Shaonan², WU Jialun², FAN Defang ²

1. Resources and Evironmental Engineering Institute, East China University of Science and Technology, Shanghai 200237, China;
2. Pesticide Environmental Toxicological Research Institute, Zhejiang University, Hangzhou 310029, China

Received:2002-10-12 Revised:2003-02-28

摘要/Abstract

摘要： 运用恒温培养法研究了阿维菌素在土壤中的降解动力学.结果表明,非生物+微生物降解、非生物降解及微生物降解的半衰期分别为34.8、277.3和49.9 d,说明阿维菌素在土壤中的降解主要由微生物引起.从试验土壤中分离到1株高效降解阿维菌素的菌株,经16S rDNA鉴定为嗜麦芽寡养单胞菌(Stenotrophomonas maltrophilia).从该降解菌中提取的粗酶液米氏常数(Km)为6.78 nmol·ml^-1,最大降解速率为81.5 nmol·min^-1·mg^-1.

关键词: 阿维菌素, 土壤, 微生物降解, 优势菌, 粗酶液

Abstract: Incubation test on the degradation dynamics of Abamectin in soil showed that the half-life of its non-biodegradation plus microbial biodegradation,non-biodegradation,and microbial biodegradation was 34.8,277.3 and 49.9 d,respectively,and its degradation in soil was mostly by microbes.A dominant bacterium which could effectively degrade Abamectin was isolated from test soil,and identified as Stenotrophomonas maltrophilia by 16S rDNA.The crude enzyme extracted from the dominant bacteria had a Michaelis-Mentn's constant 6.78 nmol穖l^-1 and a maximum rate 81.5 nmol穖in -1穖g^-1.

Key words: Abamectin, Soil, Microbial degradation, Dominant bacteria, Crude enzyme

中图分类号:

X172

张卫, 虞云龙, 林匡飞, 李少南, 吴加伦, 樊德方. 阿维菌素在土壤中的微生物降解研究[J]. 应用生态学报, 2004, (11): 2175-2178.

ZHANG Wei, YU Yunlong, LIN Kuangfei, LI Shaonan, WU Jialun, FAN Defang . Microbial degradation of Abamectin in soil[J]. Chinese Journal of Applied Ecology, 2004, (11): 2175-2178.

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