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Bt毒蛋白Cry1Ac在人造土壤中对赤子爱胜蚓毒理及生化影响

肖能文 戈峰 刘向辉   

  1. 中国科学院动物研究所 农业虫鼠害综合治理研究国家重点实验室,北京 100080
  • 收稿日期:2004-09-02 修回日期:2005-03-15 出版日期:2005-08-18

Effects of Bt toxin Cry1Ac on biochemical responses of Eisenia fetida in an artificial soil

XIAO Nengwen,GE Feng,LIU Xianghui   

  1. State Key Laboratory of Integrated Management of Pest Insects and Rodents,Institute of Zoology,Chinese Academy of Sciences,Beijing 100080,China
  • Received:2004-09-02 Revised:2005-03-15 Online:2005-08-18

摘要: Bt毒素能通过转基因作物的花粉、根和残株进入土壤.为评估转基因作物对土壤动物的影响,本文模拟转基因棉的Bt毒素进入土壤的发生程度,用含不同浓度Bt毒蛋白Cry1Ac的人造土壤处理蚯蚓,测定蚯蚓存活率、重量变化及体内总蛋白含量和过氧化氢酶(CAT)、乙酰胆碱酯酶(AchE)、谷胱甘肽?S?转移酶(GST)和纤维素酶活性.结果表明,Bt毒蛋白对蚯蚓的生物量和生理水平影响均不明显,不存在急毒性和亚致死毒性影响,对蚯蚓比较安全.

关键词: 坡耕地, 水肥效应, 水分利用效率, 黄绵土

Abstract: Bt toxin Cry1Ac could be introduced into soil by pollen,root exudates,and plant residues.To investigate its toxic effects,the Bt toxin was added into an earthworm-cultured artificial soil,and the survival,growth rate,sperm count, protein content,and activities of acetylcholinesterase (AchE),catalase (CAT), glutathione-S-transferase (GST) and cellulase in Eisenia fetida were determined. The results suggested that Bt toxin Cry1Ac had little effects on the biomass and physiological characteristics of E.fetida,with no acute and subchronic toxicity,and was safe for earthworm at field dose level.

Key words: Slopeland, Effect of soil water and fertilizer, Water use efficiency, Loess soil