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不同浓度铵态氮对镉胁迫轮叶黑藻生长及抗氧化酶系统的影响

许秋瑾;金相灿;王兴民;扈学文;陈书琴;颜昌宙   

  1. 中国环境科学研究院湖泊生态环境创新基地,国家环境保护湖泊污染控制重点实验室, 北京 100012
  • 收稿日期:2006-01-06 修回日期:2006-11-30 出版日期:2007-02-10 发布日期:2007-02-10

Effects of different concentration ammonium-N on Hydrilla verticillata antioxidant enzymes under Cd stress

XU Qiu-jin; JIN Xiang-can; WANG Xing-min; HU Xue-wen; CHEN Shu-qin; YAN Chang-zhou   

  1. State Environmental Protection Key Laboratory for Lake Pollution Control, Research Center of
    Lake Eco-environment, Chinese Research Academy of Environmental Sciences, Beijing 100012,
    China
  • Received:2006-01-06 Revised:2006-11-30 Online:2007-02-10 Published:2007-02-10

摘要: 用含有不同浓度NH4+-N (0、0.5、2.0和4.0 mg·L-1)和10 mg·L-1Cd的1/10 Hoagland营养液培养沉水植物轮叶黑藻,研究了氨态氮对Cd胁迫下轮叶黑藻的生长及抗氧化酶系统的影响,探讨富营养化污染水体沉水植物退化机理.结果表明,10 mg·L-1Cd对轮叶黑藻能产生明显的胁迫作用,叶绿素合成量明显降低;超氧化物岐化酶(SOD)与过氧化物酶(POD)活性呈先升高后下降的趋势.NH4+-N能加速Cd对植物的胁迫作用,单因子Cd作用3 h时SOD明显升高,而Cd和NH4+-N共同作用0.5 h SOD就明显升高.Cd与NH4+-N共同作用时,相对于叶绿素和蛋白质,抗氧化酶是早期敏感指标,并且SOD比POD更敏感.本试验条件下,NH4+-N与Cd共同作用2 d后,对轮叶黑藻的胁迫作用主要取决于Cd,NH4+-N的作用几乎可以忽略.

关键词: 全球危害, 防治对策, 生态系统, 生物多样性, 风险评估

Abstract: In this paper, submersed plant Hydrilla verticillata was cultivated in a 1/10 Hoagland culture medium added with different concentration NH4+-N (0, 0.5, 2.0, 4.0 mg·L-1) and 10 mg·L-1 cadmium to study the effects of NH4+-N on H. verticillata growth and its antioxidant en-zyme system under Cd stress, and approach the degradation mechanism of submersed macrophyte in eutrophic and polluted water bodies. The results showed that under 10 mg·L-1 Cd stress, the chlorophyll content of H. verticillata decreased obviously, and SOD and POD activities increased first but declined then. NH4+-N accelerated the stress effect of Cd. Under 0.5 h joint stress of Cd and NH4+-N, SOD activity increased significantly, while such an increase needed 3 hours under single Cd stress. Relative to chlorophyll and protein, antioxidant enzyme system was an early sensi-tive index, and SOD was more sensitive than POD. After two days exposure to Cd plus NH4+-N, H. verticillata was mainly stressed by Cd, and NH4+-N stress could be neglected.

Key words: Global consequences, Prevention and control strategies, Ecosystem, Biodiversity, Risk analysis