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福建家鸭品种的分子遗传多样性

李慧芳1,2;李碧春1;马月辉3;汤青萍2;陈宽维2;屠云洁2   

  1. 1扬州大学动物科学与技术学院, 江苏扬州 225009;
    2中国农业科学院家禽研究所, 江苏扬州 225003;
    3中国农业科学院畜牧研究所, 北京 100094
  • 收稿日期:2006-01-16 修回日期:2006-11-07 出版日期:2007-02-10 发布日期:2007-02-10

Molecular genetic diversity of Fujian domestic duck breeds

LI Hui-fang1,2; LI Bi-chun1; MA Yue-hui3; TANG Qing-ping2;CHEN Kuan-wei2; TU Yun-jie2   

  1. 1College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu,
    China;
    2Institute of Poultry Science,Chinese Academy of Agricultural Sciences, Yangzhou 225003, Jiangsu,
    China;
    3Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094,China
  • Received:2006-01-16 Revised:2006-11-07 Online:2007-02-10 Published:2007-02-10

摘要: 通过筛选的28个多态性较好的微卫星标记检测了福建省金定鸭、莆田黑鸭、连城白鸭、山麻鸭4个家鸭品种的遗传多样性.利用等位基因频率计算了各群体的遗传参数、群体间的Nei氏标准遗传距离DS和DA遗传距离,并采用邻近法(NJ)和类平均法(UPGMA)进行聚类分析和比较.结果表明,福建省4个家鸭品种全部群体的平均杂合度为0.5353,遗传一致性较好,应加强各保种场(区)多样性的保护;各品种间的遗传距离远近顺序在两种遗传距离DS和DA的结果中完全一致,以DA和DS为基础分别得到的UPGMA和NJ的聚类结果完全相同,表明在应用微卫星标记分析品种的遗传多样性时,使用更多的微卫星位点,才可以获得更准确更具普遍性的结论.4个家鸭品种的聚类与各品种的经济类型、生态地域分布关系密切.

关键词: 小麦进化材料, 水分利用效率, 氮利用效率

Abstract: By using 28 micro-satellite markers with better polymorphism, this paper studied the ge-netic diversity of four Fujian provincial domestic duck breeds Jinding, Putian black, Liancheng white, and Shanma. According to the alleles frequencies, the polymorphic information content, av-erage heterozygosity, anaqular genetic distance (DA) and Nei’s standard genetic distance (DS) for each breed were calculated. Based on DA and DS, four dendrograms were obtained by neighbor-joining (NJ) and UPGMA methods. The results showed that the average heterozygosity of the four duck breeds was 0.5353, indicating that the protection of the genetic diversity of these breeds should be strengthened. The orders of the two types of genetic distances among the breeds were accordant, and the dendrograms were the same, reflecting that much more micro-satellite loci should be adopted to obtain more universal conclusions when the genetic diversity was analyzed. The phy-logenetic relationships among the four duck breeds were in accordance with their economic types and ecological localities.

Key words: Wheat evolution materials, Water use efficiency, Nitrogen use efficiency