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水杨酸对高温强光胁迫下小麦叶绿体D1蛋白磷酸化及光系统Ⅱ功能的影响

马培芳1;李利红2;杨亚军1;赵会杰1;付晓记1;张超男1   

  1. 1河南农业大学生命科学学院, 郑州 450002; 2郑州牧业工程高等专科学校, 郑州 450001
  • 收稿日期:2008-05-20 修回日期:1900-01-01 出版日期:2008-12-20 发布日期:2008-12-20

Effects of salicylic acid on D1 protein phosphorylation and PSⅡ performance in wheat leaf chloroplasts under high temperature and high light stress.

MA Pei-fang1;LI Li-hong2 ;YANG Ya-jun1;ZHAO Hui-jie1;FU Xiao-ji1;ZHANG Chao-nan1   

  1. 1College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China;2Zhengzhou College of Animal Husbandry Engineering, Zhengzhou 450001, China
  • Received:2008-05-20 Revised:1900-01-01 Online:2008-12-20 Published:2008-12-20

摘要: 为了研究水杨酸(SA)对高温强光胁迫下小麦叶片类囊体膜D1蛋白磷酸化和PSⅡ功能的影响,用0.5 mmol·L-1 SA溶液预处理灌浆期小麦叶片,以水预处理为对照,然后将预处理植株进行高温强光(35 ℃,1 600 μmol·m-2·s-1)处理,测定胁迫处理过程中小麦旗叶光合电子传递速率、净光合速率、叶绿素荧光参数及D1蛋白的变化.结果表明:SA预处理有效抑制了高温强光下D1蛋白的净降解,保持了较高的D1蛋白磷酸化水平、全链电子传递速率和PSⅡ电子传递速率,维持了较高的PSⅡ原初光化学效率(Fv/Fm)、实际光化学效率(ФPSⅡ)、光化学淬灭系数(qP)和净光合速率(Pn).表明外源SA通过调节小麦叶绿体D1蛋白的周转,减轻了高温强光胁迫对叶片光合机构的损伤,有利于PSⅡ的正常运转.

关键词: 光合特性, 遮阴, 叶绿素, 紫堇属, 叶绿素荧光

Abstract: To study the effects of salicylic acid (SA) on the D1 protein phosphorylation and PSⅡ performance in wheat (Tritivum aestivum L.) leaf chloroplasts under high temperature and high light, the wheat leaves at grain-filling stage were sprayed with SA solution (0.5 mmol·L-1) or water (as control), and then subjected to 35 ℃ and 1 600 μmol·m-2·s-1 for various hours. The changes in electron transport rate (ETR), net photosynthetic rate, chlorophyll fluorescence parameters, and relative amount of phosphorylated and nonphosphorylated D1 protein in thylakoid membrane were compared. The results showed that foliar spraying SA effectively inhibited the degradation of D1 protein under high temperature and high light stress, and maintained the D1 protein phosphorylation, ETR of whole chain and PSⅡ, Fv/Fm (the maximal photochemical efficiency of PSⅡ), ФPSⅡ (the actual photochemical efficiency of PSⅡ), qP (the photochemical quenching coefficient), and Pn (net photosynthetic rate) at a higher level, indicating that exogenous SA could mitigate the damage effect of high temperature and high light on wheat leaf photosynthetic apparatus and benefit PSⅡperformance via regulating the turnover of D1 protein in chloroplasts.

Key words: photosynthetic characteristics, shading, chlorophyll fluorescence, Corydalis, chlorophyll