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绿僵菌大孢变种的生物学特征及其对蛴螬的毒力研究

林华峰;李世广;张磊;王萍莉;周义文   

  1. 安徽农业大学植保学院,合肥 230036
  • 收稿日期:2005-04-21 修回日期:2005-10-20 出版日期:2006-02-18 发布日期:2006-02-18

Biological characteristics of Metarhizium anisopliae var.major and its virulence to white grubs

LIN Huafeng;LI Shiguang;ZHANG Lei;WANG Pingli;ZHOU Yiwen   

  1. School of Plant Protection,Anhui Agricultural University,Hefei 230036,China
  • Received:2005-04-21 Revised:2005-10-20 Online:2006-02-18 Published:2006-02-18

摘要: 以报道较多的蛴螬病原真菌布氏白僵菌、绿僵菌小孢变种作参照菌,系统研究了新分离鉴定的绿僵菌大孢变种的生物学特性,测定了其在偏低的自然气温下对蛴螬的致病能力.结果表明,绿僵菌大孢变种与参照菌的培养要求接近,最适宜的温度为25 ℃.3种供试的培养基中,PPDA为最适宜的培养基,其次为SDAY.在偏低的温度环境下绿僵菌大孢变种对蛴螬的毒杀能力强于布氏白僵菌,土壤和喷雾处理后累计僵虫率分别达88.23%和76.47%,有很好的田间应用前景.

关键词: 干旱胁迫, 基因表达, ABA, 信号转导, 转基因植物

Abstract: With Beauveria brongniartii and Metarhizium anisopliae var.anisopliae as the reference,this paper studied the biological characteristics and invading ability of Metarhizium anisopliae var.major,a new variety separated from white grubs.The results showed that the optimal culture condition of M.anisopliae var.major was similar with that of other entomopathogenic species,and the optimal temperature was 25 ℃.Among the three test media,PPDA was the most appropriate one,followed by SDAY and Czapek.M.anisopliaevar.major had a stronger virulence than B.brongniartii at lower temperature,with an accumulative mortality of white grubs being 88.23% and 76.47%,respectively,after treated with soil and through spraying,suggesting that M.anisopliae var.major had a good prospect to widely apply in field.

Key words: Drought stress, Gene expression, ABA, Signaling transduction, Transgenic plant