长白山苔原植物深色有隔内生真菌物种多样性

doi:10.13287/j.1001-9332.202406.029

应用生态学报 ›› 2024, Vol. 35 ›› Issue (6): 1689-1694.doi: 10.13287/j.1001-9332.202406.029

长白山苔原植物深色有隔内生真菌物种多样性

苏丹¹, 孙蕊¹, 许榕凡¹, 张文浩¹, 吕国忠^2*

¹沈阳大学生命科学与工程学院, 沈阳 110044;
²大连民族大学环境与资源学院, 辽宁大连 116600

收稿日期:2023-11-01 接受日期:2024-03-25 出版日期:2024-06-18 发布日期:2024-12-18
通讯作者: ^*E-mail: lvgz@dlnu.edu.cn
作者简介:苏丹, 女, 1984年生, 副教授。主要从事微生物资源与利用等研究。E-mail: sdsyau@163.com
基金资助:
国家自然科学基金项目(3177011075)、中国国家留学基金项目(202008210100)、辽宁省教育厅青年育苗计划项目(2020JYT02)、生物技术与资源利用教育部重点实验室开放课题(KF2021002)、2021年辽宁省教育厅青年扶持项目和辽宁省普通本科高等学校校际合作项目

Species diversity of dark septate endophytes in tundra plants of Changbai Mountains, Northeast China

SU Dan¹, SUN Rui¹, XU Rongfan¹, ZHANG Wenhao¹, LYU Guozhong^2*

¹College of Life Science and Engineering, Shenyang University, Shenyang 110044, China;
²College of Environment and Resources, Dalian Minzu University, Dalian 116600, Liaoning, China

Received:2023-11-01 Accepted:2024-03-25 Online:2024-06-18 Published:2024-12-18

摘要/Abstract

摘要： 从长白山自然保护区苔原带典型植物牛皮杜鹃、毛毡杜鹃、笃斯越橘、仙女木的根系分离获得深色有隔内生真菌(DSE)菌株,研究其侵染定殖情况。采用形态特征与rDNA ITS序列分析相结合进行分类鉴定,研究了4种苔原植物的DSE真菌群落组成和物种多样性。结果表明: 4种苔原植物的根系内均形成DSE的典型结构“微菌核”。共分离出69株DSE菌株,分属于10个属12个种,分别是福廷瓶头霉、链格孢、细极链格孢、黑附球菌、小孢金丝酵母、白蜡树炭疽菌、茶树炭疽菌、小球腔菌属、枝状枝孢霉、茎点霉属、淡领瓶霉属、 Discosia italica,具有丰富的物种多样性,且所有种均为我国高山苔原带首次报道的DSE真菌,其中福廷瓶头霉为各苔原植物的共有种和优势种。4种苔原植物DSE真菌的Simpson、Pielou和 Shannon多样性指数均差异显著。表明苔原植物具有丰富的DSE真菌多样性,且二者能够形成良好的共生关系,增强苔原植物对环境的适应性。

关键词: 深色有隔内生真菌, 侵染特征, 群落组成, 物种多样性

Abstract: We isolated the dark septate endophytic (DSE) fungi from roots of typical plant species in the tundra of Changbai Mountains Nature Reserve, including Rhododendron aureum, R. conferentiatum, Vaccinium uliginosum, and Dryas octopetala, and studied their colonization. We further investigated the DSE community composition and species diversity of the four tundra plant species by using morphological characteristics combined with rDNA ITS sequence analysis. The results showed that DSE formed a typical structure of “microsclerotia” in roots of the four plant species. A total of 69 strains of DSE fungi were isolated from the root samples, belonging to 10 genera, and 12 species. They were Phialocephala fortinii, Alternaria alternata, A. tenuissima, Epicocum nigrum, Canariomyces microsporus, Colletotrichum spaethianum, C. camelliae, Leptophoria sp., Cladosporium cladosporioides, Phoma sp., Cadophora sp., and Discosia italica, respectively. The DSE fungal species diversity was rich, and all these fungal species were firstly reported as DSE fungi in the alpine tundra belt of China. Among them, Phialocephala fortinii was the common and dominant species of all tundra plants. The Simpson, Pielou, and Shannon diversity indices of DSE fungi of the four plant species of tundra differed significantly. Our results showed that tundra plants have rich diversity of DSE fungi, and they can form a good symbiotic relationship, which enhance the adaptability of tundra plants to the harsh environment.

Key words: dark septate endophytic fungi, infection characteristics, community composition, species diversity

苏丹, 孙蕊, 许榕凡, 张文浩, 吕国忠. 长白山苔原植物深色有隔内生真菌物种多样性[J]. 应用生态学报, 2024, 35(6): 1689-1694.

SU Dan, SUN Rui, XU Rongfan, ZHANG Wenhao, LYU Guozhong. Species diversity of dark septate endophytes in tundra plants of Changbai Mountains, Northeast China[J]. Chinese Journal of Applied Ecology, 2024, 35(6): 1689-1694.

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长白山苔原植物深色有隔内生真菌物种多样性

Species diversity of dark septate endophytes in tundra plants of Changbai Mountains, Northeast China

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