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应用生态学报 ›› 1990, Vol. 1 ›› Issue (2): 172-176.

• 研究论文 • 上一篇    下一篇

红松越冬针叶的电子自旋共振(ESR)波谱

靳月华1, 陶大立1, 杜英君1, 王淼1, 高均成2, 杜亚经2   

  1. 1. 中国科学院沈阳应用生态研究所, 沈阳 110015;
    2. 中国计量科学院, 北京 100013
  • 收稿日期:1989-07-10 出版日期:1990-04-25 发布日期:1990-04-25
  • 基金资助:

    国家自然科学基金资助课题

Electron spin resonance spectrum from overwintered korean pine seedlings

Jin Yuehua1, Tao Dali1, Du Yingjun1, Wang Miao1, Gao Juneheng2, Du Yiajin2   

  1. 1. Institute of Applied Ecology, Academia Siniea, Shenyang 110015;
    2. Chinese Academy of Measurement, Beijing 100013
  • Received:1989-07-10 Online:1990-04-25 Published:1990-04-25

摘要: 用ESR波谱仪在越冬红松针叶及叶绿体冻干粉中检出了g值分别为2.0044和2.0037的明确的单峰信号。针叶伤害愈重,信号愈强。此信号可被Vc或富含SOD等多种自由基清除剂的沙棘果汁所抑制。针叶受害愈重,沙棘果汁对信号的抑制作用愈强,Vc的抑制作用愈弱。据此认为红松苗越冬伤害和有机自由基的形成有关。在伤害发展的不同阶段,有机自由基的种类组成也不同。

关键词: 有机自由基, ESR, 红松, 叶绿体, 类囊体, 光氧化

Abstract: Single-peaked ESRsignals with g factor 2.0044 and 2.0037 were clearely shown respecti vely from overwintered korean pine needles and their lyophylized chloroplasts. The more serious the injury of the needles, the stronger the signal. The signals were diminished by adding Vc or fruit juice of Elaeagnus angustifolia containing rich SODand other free-radical scavengers.The more serious the injury, the more effective of the fruit juice, but Jess of Vc in inhibiting the ESRsignals. The results indicate that the winter injury of korean pine seedlings is related to the formation of organic free radicals. The major kinds of the radicals are differ in advancing stages of the injury development.

Key words: Organic free radicals, ESR, Pinus koraiensis, Chloroplast, Thylakoid, Photooxi dation