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应用生态学报 ›› 1995, Vol. 6 ›› Issue (4): 406-410.

• 研究论文 • 上一篇    下一篇

用水生开花植物作指示植物的生物测定法──水和土壤水溶液中西玛津的测定

区自清, 任丽萍, 贾良清, 张海荣, 常士俊, 孙铁珩   

  1. 中国科学院沈阳应用生态研究所陆地生态系统痕量物质生态过程开放实验室, 沈阳 110015
  • 收稿日期:1995-01-25 修回日期:1995-06-23 出版日期:1995-10-25 发布日期:1995-10-25
  • 基金资助:

    中国科学院陆地生态系统痕量物质生态过程开放实验室基金

Bioassay of simazine in water and soil water solution using aquatic flowering plants as indicators

Ou Ziqing, Ren Liping, Jia Liangqing, Zhang Hairong, Chang Shijun, Sun Tieheng   

  1. Laboratory of of Ecological Process of Trace Substance in Terrestrial Ecosystem, Institute of Applied Ecology, Academia Sinica, Shenyang 110015
  • Received:1995-01-25 Revised:1995-06-23 Online:1995-10-25 Published:1995-10-25

摘要: 建立了一个简单、快速、灵敏的,用水生开花植物作指示植物的生物测定法,可用于水和土壤溶液中有效态光合作用抑制型除草剂(西玛津)的测定。该方法是根据西玛津对植物光合作用氧释放的影响,用氧电极直接测定溶液中溶解氧来计算西玛津的含量。作用10min即可测定,西玛津的最低检出浓度可达0.02mg·L-1.五刺金鱼藻、比毛莨和泽泻更适于作指示植物。其平均口收率为96-100%,与C18柱提取/HPLC法的平均回收率(98-100%)非常接近,测定结果几乎完全一致。

关键词: 生物测定, 水生开花植物, 西玛津, 除草剂, 水稻, 镉污染, 稻米镉含量, 碱性肥料

Abstract: A simple, rapid and sensitiye bioassay using aquatic flowering plants as indicators is used for measuring available photosynthesis-inhibiting herbicides (simazine) in water and soil water solution.This method is based on the effect of simazine on oxygen release of photosynthesis, which can be directly measured using an oxygen electrode, and with which, the amount of simazine can be calculated.Measurement can be made within 10 min of treatment.This method is capable of measuring a minimum simazine concentration of 0.02mg·L-1.Ceratophyllum oryzetorum is a better indicator than Ranunculus trichophyllus and Alisma planlago-aquatica.The mean recoveries of simazine by bioassay using C.oryzetorum are 96 to 100%, which is comparable to those (98 to 100%) obtained by C18 column extraction and HPLC measurement.

Key words: Bioassay, Aquatic flowering plant, Simazine, Herbicide, Cd pollution, rice, alkaline fertilizer, Cd concentration of rice