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应用生态学报 ›› 1995, Vol. 6 ›› Issue (s1): 88-92.

• 研究论文 • 上一篇    下一篇

我国仓贮昆虫病原芽孢杆菌的研究

陈涛, 刘勇, 杨冬菊, 王春生, 夏克祥, 侯建文   

  1. 中国科学院武汉病毒研究所, 武汉430071
  • 收稿日期:1993-09-16 修回日期:1994-03-15 出版日期:1995-12-25 发布日期:1995-12-25
  • 基金资助:

    国家自然科学基金资助项目

Study on insect pathogenic Bacilli in store ecotope of China

Chen Tao, Liu Yong, Yang Dongju, Wang Chunshen, Xia Kexiang   

  1. Wuhan Institute of Virology,Academic Sinica,Wuhan 430071
  • Received:1993-09-16 Revised:1994-03-15 Online:1995-12-25 Published:1995-12-25

摘要: 对951个样品分离鉴定,有747个样品含芽孢杆菌,有菌率为78.55%.共分离得到芽孢杆菌1138株,其中苏云金杆菌(Bacillus thuringiensis,简称B.t)143株,占12.5%;球形芽孢杆菌(Bacillus sphaericus,简称B.s)11株,占0.97%;其他芽孢杆菌984株,占86.40%.从芽孢杆菌中选出产生晶体、苏云金素或磷酸酯酶C(Phosphalipase C,简称PLC)的毒素菌株158株,其中B.t占143株,B.s有5株,其他芽孢杆菌10株.在产毒素菌株中,经测定有120株菌对供试昆虫毒性达标.占77.92%.不同菌株的杀虫毒素、杀虫范围和毒力各异,认为这种差异取决于毒素和虫种两方面的特异性.

关键词: 仓贮生态环境, 芽孢杆菌, 杀虫毒素, 杀虫毒性, 凋落叶分解, 长白山, 底栖动物, 冻结初期, 凋落叶混合效应

Abstract: Identification of 951 samples collected from different store ecotope shows that 747 samples of them have the Bacillus. 1138 strains of the Bacillus were isolated from 951 samples,including 143 strains of B.t (12.5%), 11 strains of B,s (0.97%) and 984 strains ofother Bacilli (86.40%). 158 strains of thuringionsin, phosphalipase C and parasporalcrystals were separated from 1138 store strains. Among 158 strains,there were 143 strainsof B. t, 5 strains of B. s and 10 strains of other Bacilli. In total of 158 toxin strains, 120strains could kill insect. It is showed that relativity exist between insecticidal toxin andtoxicity in the strains. But insecticidal toxins of different Bacillus are different in insecticidal range and toxicity.

Key words: Store ecotope, Bacillus, Insecticidal toxin, Insecticidal toxicity, Changbai Mountains, litter mixing effect, litter decomposition, early frost period, benthic macroinvertebrate