速效氮肥配施双氰胺对减控小白菜镉积累的作用

doi:10.13287/j.1001-9332.202009.035

应用生态学报 ›› 2020, Vol. 31 ›› Issue (9): 3093-3100.doi: 10.13287/j.1001-9332.202009.035

速效氮肥配施双氰胺对减控小白菜镉积累的作用

攸越¹, 吴蔡楠¹, 钟苇杰¹, 侯之琳¹, 刘越¹, 都韶婷^1,2*, 金崇伟³

¹浙江工商大学环境科学与工程学院, 杭州 310018;
²浙江工商大学分析测试中心, 杭州 310018;
³浙江大学环境与资源学院, 杭州 310008

收稿日期:2019-12-24 接受日期:2020-06-10 出版日期:2020-09-15 发布日期:2021-03-15
通讯作者: * E-mail: dushaoting@zjgsu.edu.cn
作者简介:攸越, 女, 1997年生, 硕士研究生。主要从事重金属污染土壤安全生产研究。E-mail: 1621881555@qq.com
基金资助:
国家重点研发计划项目(2016YFD0200103)和国家自然科学基金项目(41977145)资助

Effects of dicyandiamide on cadmium accumulation in pakchoi under instant soluble nitrogen fertilizers

YOU Yue¹, WU Cai-nan¹, ZHONG Wei-jie¹, HOU Zhi-lin¹, LIU Yue¹, DU Shao-ting^1,2*, JIN Chong-wei³

¹College of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310018, China;
²Instrumental Analysis Center, Zhejiang Gongshang University, Hangzhou 310018, China;
³College of Environment and Resources, Zhejiang University, Hangzhou 310008, China

Received:2019-12-24 Accepted:2020-06-10 Online:2020-09-15 Published:2021-03-15
Contact: * E-mail: dushaoting@zjgsu.edu.cn
Supported by:
the Natural Key R&D Program of China (2016YFD0200103) and the National Natural Science Foundation of China (41977145).

摘要/Abstract

摘要： 本文以小白菜为试材,研究了镉(Cd)污染土壤中配施双氰胺(DCD)对不同速效氮肥(硫酸铵,简称全铵;硝酸钠,简称全硝;11硫酸铵和硝酸钠,简称硝铵;尿素)下小白菜生长及Cd含量的影响。结果表明: 与对照相比,Cd污染土壤中配施DCD使全铵、硝铵和尿素处理下的小白菜可食部分鲜重分别增加583.3%、41.5%和206.8%,耐受系数(TI)和光合作用速率均大幅度提高;全硝处理则无明显变化。配施DCD使各氮肥处理下小白菜叶片的丙二醛、过氧化氢含量和超氧自由基产生速率均不同程度地下降,且以全铵和尿素处理的下降幅度最大。与对照相比,全铵、硝铵和尿素处理的小白菜叶片的Cd含量分别减少58.3%、34.0%和44.5%;叶柄的Cd含量分别减少61.8%、29.4%和55.6%。全硝处理下小白菜叶片和叶柄的Cd含量与对照相比无明显差异。配施DCD不同程度降低了各处理根际土壤的酸化可能是造成上述结果的主要原因。在缓冲能力较弱的Cd污染土壤中,施用全铵、硝铵和尿素氮肥时配施DCD可以缓解Cd污染土壤对小白菜生长的胁迫,并有效减少Cd在植物体内的积累。

关键词: 镉, 土壤pH, 耐受系数, 硝化抑制剂, 氧化胁迫, 氮肥

Abstract: We investigated the effects of dicyandiamide (DCD) on the growth and Cd concentrations in pakchoi cultivated under different instant soluble N fertilizers [ammonium sulfate, ammonium sulfate and sodium nitrate (1:1, ammonium/nitrate), and urea] in Cd-contaminated soils. The results showed that the fresh weight of the edible parts of Cd-stressed pakchoi were increased by 583.3%, 41.5%, and 206.8% under ammonium, ammonium/nitrate, and urea treatments in the presence of DCD, respectively compared with control, and the tolerance index and photosynthetic rate significantly increased, whereas no significant changes were observed under nitrate supply. Under all N treatments with DCD, the MDA and H₂O₂ contents and the superoxide radical production rate in the leaves of pakchoi were decreased, with the highest reduction occurred in ammonium and urea treatments. Cd concentrations in the leaves of pakchoi fertilized with ammonium, ammonium/nitrate, and urea were lowered by 58.3%, 34.0%, and 44.5% and those in the petioles were lowered by 61.8%, 29.4%, and 55.6%, respectively. Cd concentration in the leaves and petioles of pakchoi in the nitrate treatment did not differ significantly from control. These changes could be attributable to the reduction in the acidification of rhizosphere soil in response to the combined application of N fertilizer and DCD. Accordingly, in Cd-contaminated soils with a low buffering capacity, the application of DCD combined with ammonium, ammonium/nitrate, or urea N fertili-zers could alleviate Cd-induced growth stress and inhibit photosynthesis in pakchoi plants and effectively minimize the Cd accumulation.

Key words: cadmium, soil pH, tolerance index, nitrification inhibitor, oxidative stress, nitrogen fertilizer

攸越, 吴蔡楠, 钟苇杰, 侯之琳, 刘越, 都韶婷, 金崇伟. 速效氮肥配施双氰胺对减控小白菜镉积累的作用[J]. 应用生态学报, 2020, 31(9): 3093-3100.

YOU Yue, WU Cai-nan, ZHONG Wei-jie, HOU Zhi-lin, LIU Yue, DU Shao-ting, JIN Chong-wei. Effects of dicyandiamide on cadmium accumulation in pakchoi under instant soluble nitrogen fertilizers[J]. Chinese Journal of Applied Ecology, 2020, 31(9): 3093-3100.

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速效氮肥配施双氰胺对减控小白菜镉积累的作用

Effects of dicyandiamide on cadmium accumulation in pakchoi under instant soluble nitrogen fertilizers

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