干旱胁迫下华北落叶松幼苗接种木霉的生理变化

doi:10.13287/j.1001-9332.202103.004

应用生态学报 ›› 2021, Vol. 32 ›› Issue (3): 853-859.doi: 10.13287/j.1001-9332.202103.004

干旱胁迫下华北落叶松幼苗接种木霉的生理变化

孙悦燕, 王秀丽, 高润梅^*, 李进

山西农业大学林学院, 山西晋中 030801

收稿日期:2020-08-03 接受日期:2021-01-03 出版日期:2021-03-15 发布日期:2021-09-15
通讯作者: * E-mail: sxndgrm@163.com
作者简介:孙悦燕, 女, 1983年生, 硕士研究生。主要从事森林生态学及植物生理生态学研究。E-mail: sunyueyan_20081108@163.com
基金资助:
国家自然科学基金项目(31400536)、山西省回国留学人员科研资助项目(2015-062)和山西省林业科技创新项目(LYCX201961)资助

Physiological changes of Larix principis-rupprechtii seedlings inoculated with Trichoderma spp. under drought stress

SUN Yue-yan, WANG Xiu-li, GAO Run-mei^*, LI Jin

College of Forestry, Shanxi Agricultural University, Jinzhong 030801, Shanxi, China

Received:2020-08-03 Accepted:2021-01-03 Online:2021-03-15 Published:2021-09-15
Contact: * E-mail: sxndgrm@163.com
Supported by:
National Natural Science Foundation of China (31400536), the Research Project Supported by Shanxi Scholarship Council of China (2015-062), and the Forestry Science and Technology Innovation Project of Shanxi Province (LYCX201961)

摘要/Abstract

摘要： 水分匮缺显著影响林木更新,根际接种木霉可减缓林木所受干旱胁迫的影响。本研究通过人工控水试验,分析了根际接种木霉对干旱条件下(轻度、中度和重度干旱,培养基质相对含水量分别为50%～60%、35%～50%、20%～35%)华北落叶松幼苗针叶抗氧化系统和渗透物质的生理影响。结果表明: 随着干旱胁迫加剧,幼苗针叶的超氧化物歧化酶(SOD)和过氧化物酶(POD)活性显著增强,丙二醛和脯氨酸含量持续上升,可溶性蛋白质和淀粉含量呈现下降的趋势。接种木霉后,干旱胁迫下幼苗针叶的SOD和POD活性增幅更大,丙二醛含量上升幅度减小。中度干旱条件下,接菌苗的POD活性是未接菌苗的1.8倍,丙二醛含量是未接菌苗的62.9%。接菌后,干旱胁迫下幼苗针叶的脯氨酸、可溶性蛋白质、可溶性糖和淀粉含量均高于未接菌幼苗。根际接种木霉后,受干旱胁迫的华北落叶松幼苗的抗氧化系统和渗透物质调节能力增强,幼苗对干旱胁迫的耐受性增强。在华北地区气候日趋暖干化的趋势下,可将根际接种木霉技术推广用于华北落叶松人工造林或林下抚育。

关键词: 华北落叶松, 木霉, 干旱, 生理变化

Abstract: Tree regeneration is significantly affected by water deficiency, which could be alleviated by the inoculation of Trichoderma spp. In this study, the effects of rhizosphere inoculation with Trichoderma spp. on the antioxidant system and osmotic substances in the seedlings of Larix principis-rupprechtii was investigated under experimental drought stresses, with the relative water content 50%-60%, 35%-50%, and 20%-35% as the light, moderate and severe drought stress, respectively. The results showed that, with increasing drought stress, the activities of protective enzymes, SOD and POD in the needles of seedlings were significantly promoted, the contents of MDA and proline were remarkably increased, whereas the contents of soluble protein and starch showed a decreasing trend. Inoculation with Trichoderma spp. promoted much stronger increase in the activities of SOD and POD in the needles, yet the MDA content showed a lower level of increase. Under moderate drought, the activity of POD in the seedling inoculated with Trichoderma spp. was 1.8 folds of that in the control, while the content of MDA was 62.9% of the control. The contents of proline, soluble protein, soluble sugar, and starch in the inoculated seedlings were higher than that in the uninoculated seedlings. Drought-resistance of L. principis-rupprechtii seedlings inoculated by Trichoderma spp. was significantly enhanced due to the augmenting regulation of antioxidant system and osmotic adjustment substances. Therefore, under the trend of warming and drying in North China, the technique of rhizosphere inoculation of Trichoderma spp. could be popularized for forest plantation or understory tending.

Key words: Larix principis-rupprechtii, Trichoderma spp., drought, physiological change

孙悦燕, 王秀丽, 高润梅, 李进. 干旱胁迫下华北落叶松幼苗接种木霉的生理变化[J]. 应用生态学报, 2021, 32(3): 853-859.

SUN Yue-yan, WANG Xiu-li, GAO Run-mei, LI Jin. Physiological changes of Larix principis-rupprechtii seedlings inoculated with Trichoderma spp. under drought stress[J]. Chinese Journal of Applied Ecology, 2021, 32(3): 853-859.

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干旱胁迫下华北落叶松幼苗接种木霉的生理变化

Physiological changes of Larix principis-rupprechtii seedlings inoculated with Trichoderma spp. under drought stress

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