种植模式对当归根际细菌群落多样性及代谢通路的影响

doi:10.13287/j.1001-9332.202112.031

应用生态学报 ›› 2021, Vol. 32 ›› Issue (12): 4254-4262.doi: 10.13287/j.1001-9332.202112.031

种植模式对当归根际细菌群落多样性及代谢通路的影响

姜小凤^1,2, 郭凤霞^1*, 陈垣¹, 郭建国², 刘晓峰¹

¹甘肃农业大学生命科学技术学院/农学院/甘肃省干旱生境作物学重点实验室/甘肃省中药材规范化生产技术创新重点实验室/甘肃省药用植物栽培育种工程研究中心, 兰州 730070;
²甘肃省农业科学院旱地农业研究所/植物保护研究所, 兰州 730070

收稿日期:2021-05-07 修回日期:2021-08-27 出版日期:2021-12-15 发布日期:2022-06-15
通讯作者: *E-mail: guofx@gsau.edu.cn
作者简介:姜小凤, 女, 1974年生, 副研究员, 博士研究生. 主要从事土壤生态及药用植物栽培研究. E-mail: jxf_5188@163.com
基金资助:
甘肃省农业科学院科技创新专项(当归绿色高效栽培技术模式研究与示范 2021GAAS11)、国家自然科学基金项目(31560175,31360317,31460547)、甘肃省现代农业中药材产业体系首席专家项目(GARS-ZYC-1)、国家中医药管理局中药材标准化建设项目(ZYBZH-Y-GS-11)和大学生创新训练项目(201810733232,20150802,20160802,20170924)资助

Effect of cropping patterns onbacterial community diversity and metabolic function in rhizosphere soil of Angelica sinensis

JIANG Xiao-feng^1,2, GUO Feng-xia^1*, CHEN Yuan¹, GUO Jian-guo², LIU Xiao-feng¹

¹College of Life Science and Technology/College of Agronomy/Gansu Provincial Key Laboratory of Aridland Crop Science/Gansu Provincial Key Laboratory of Good Agricultural Production for Traditional Chinese Medicines/Gansu Provincial Engineering Research Centre for Medical Plant Cultivation and Breeding,Gansu Agricultural University, Lanzhou 730070, China;
²Dryland Agriculture Institute/Institute of Plant Protection, Gansu Academy of Agricultural Sciences,Lanzhou 730070, China

Received:2021-05-07 Revised:2021-08-27 Online:2021-12-15 Published:2022-06-15
Contact: *E-mail: guofx@gsau.edu.cn
Supported by:
Gansu Academy of Agricultural Sciences Science and Technology Innovation Special Project (Research and Demonstration on Green and Efficient Cultivation Model of Angelica sinensis 2021GAAS11) , the National Natural Science Foundation of China (31560175, 31360317, 31460547), the Chief Expert Project of Modern Agricultural Traditional Chinese Medicine Industry System in Gansu Province (GARS-ZYC-1), the National Standardization Project of Traditional Chinese Medicine of China (ZYBZH-Y-GS-11), and Innovation Training Program for College Students (201810733232, 20150802, 20160802, 20170924)

摘要/Abstract

摘要： 连作障碍严重限制了当归产业的可持续发展。为了探索当归(Angelica sinensis)高效栽培技术,本研究在当归主产区甘肃省渭源县设置5种种植模式(A: 豌豆-当归-当归,对照;B: 豌豆-小麦-当归;C: 豌豆-蒙古黄芪-当归;D: 豌豆-马铃薯-当归;E: 豌豆-休耕-当归),于采挖期分别测定不同种植模式下,当归根际土壤理化特性和细菌基因组DNA相对丰度,分析不同种植模式对当归根际土壤理化特性、细菌群落多样性和代谢通路的影响。结果表明: 1) 5种种植模式下当归根际土壤理化特性差异较大。与对照相比,C模式根际土壤的电导率显著增加,B、D和E模式的电导率略有下降,B、C、D和E模式的土壤CO₂呼吸速率显著提高。2) 5种种植模式的当归根际土壤细菌隶属于26门368属,其中,芽单胞菌门的芽单胞菌属、变形菌门的鞘脂单胞菌属和酸杆菌门的Subgroup_6属为优势菌属。与对照相比,B、C模式变形菌门和放线菌门的相对丰度显著增加,D模式酸杆菌门的相对丰度显著降低;E模式变形菌门、酸杆菌门和放线菌门的相对丰度显著增加。3) 5种种植模式下,当归根际土壤的pH值、电导率、有机质、碱解氮、有效磷和速效钾含量与变形菌门细菌的相对丰度呈显著负相关。4) 5种种植模式下,当归根际土壤中6种代谢通路细菌的相对丰度差异显著。C模式对当归根际土壤理化特性和细菌群落有较好的调节作用,是克服当归连作障碍的主要种植模式。

关键词: 种植模式, 当归, 根际土壤, 理化特性, 细菌群落, 代谢通路

Abstract: The sustainable development of Angelica sinensis industry is seriously restricted by continuous cropping obstacles. In order to explore an efficient cultivation technique for A. sinensis, an experiment with five cropping patterns [A: Pisum sativum (Ps)-A. sinensis (As)-As, control); B: Ps-Triticum aestivum (Ta)-As; C: Ps-Mongolia astragalus (Ma)-As; D: Ps-Solanum tuberosum (St)-As); E: Ps-Fallow (F)-As)] were conducted in major A. sinensis producing areas located in Weiyuan County, Gansu Province. The physicochemical properties and relative abundance of bacterial genomic DNA in rhizosphere soil under different cropping patterns were measured during A. sinensis harvest period to investigate the effects of different cropping patterns on physicochemical properties, bacterial community diversity, and metabolic pathways. The results showed that: 1) the physicochemical properties in A. sinensis rhizosphere soil varied among different cropping patterns. Compared with the control, soil electrical conductivity under C pattern was significantly higher, and lower under B, D and E, CO₂ respiration rate for B, C, D and E were significantly increased. 2) Soil bacteria of A. sinensis rhizosphere soil in the five cropping patterns belonged to 26 phyla and 368 genera. The dominant genera were Gemmatimonas from Gemmatimonadetes, Sphingomonas from Proteobacteria, and Subgroup_6 from Acidobacteria. Compared with the control, the relative abundance of Proteobacteria and Actinobacteria under B and C patterns was significantly higher, Acidobacteria in D pattern was significantly lower, while Proteobacteria, Acidobacteria, and Actinobacteria in E pattern was significantly higher. 3) There were significantly negative relations between soil pH, electrical conductivity, contents of organic matter, available nitrogen, phosphorus and potassium with the relative abundance of Proteobacteria in A. sinensis rhizosphere soil across the five cropping patterns. 4) There was significant difference in relative abundance for bacteria of six metabolic pathways under the five cropping patterns. In conclusion, C pattern had a regulating effect on physicochemical properties and bacterial communities in A.sinensis rhizosphere soil, which could be taken as a major practice to overcome the continuous cropping obstacles.

Key words: cropping patterns, Angelica sinensis, rhizosphere soil, physicochemical properties, bacteria communities, metabolic pathways

姜小凤, 郭凤霞, 陈垣, 郭建国, 刘晓峰. 种植模式对当归根际细菌群落多样性及代谢通路的影响[J]. 应用生态学报, 2021, 32(12): 4254-4262.

JIANG Xiao-feng, GUO Feng-xia, CHEN Yuan, GUO Jian-guo, LIU Xiao-feng. Effect of cropping patterns onbacterial community diversity and metabolic function in rhizosphere soil of Angelica sinensis[J]. Chinese Journal of Applied Ecology, 2021, 32(12): 4254-4262.

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种植模式对当归根际细菌群落多样性及代谢通路的影响

Effect of cropping patterns onbacterial community diversity and metabolic function in rhizosphere soil of Angelica sinensis

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