氟喹诺酮类抗生素的环境污染及其对微生物介导氮循环的影响

doi:10.13287/j.1001-9332.202311.032

应用生态学报 ›› 2023, Vol. 34 ›› Issue (11): 3114-3126.doi: 10.13287/j.1001-9332.202311.032

氟喹诺酮类抗生素的环境污染及其对微生物介导氮循环的影响

温丽联^1,3, 宋金明^1,2,3,4*, 李学刚^1,2,3,4, 马骏^1,4, 戴佳佳^1,4, 袁华茂^1,2,3,4, 段丽琴^1,2,3,4, 王启栋^1,4

¹中国科学院海洋研究所, 中国科学院海洋生态与环境科学重点实验室, 山东青岛 266071;
²青岛海洋科学与技术试点国家实验室海洋生态与环境科学功能实验室, 山东青岛 266237;
³中国科学院大学, 北京 100049;
⁴中国科学院海洋大科学研究中心, 山东青岛 266071

收稿日期:2023-07-03 修回日期:2023-09-21 出版日期:2023-11-15 发布日期:2024-05-15
通讯作者: *E-mail: jmsong@qdio.ac.cn
作者简介:温丽联, 女, 1994年生, 博士研究生。主要从事人工合成药物的海洋生物地球化学过程研究。E-mail: wenlilian@qdio.ac.cn
基金资助:
中国科学院A类先导专项项目(XDA23050501)和中国科学院特别研究助理资助项目

Environmental pollution of fluoroquinolones and its relationship with nitrogen cycling mediated by microorganisms.

WEN Lilian^1,3, SONG Jinming^1,2,3,4*, LI Xuegang^1,2,3,4, MA Jun^1,4, DAI Jiajia^1,4, YUAN Huamao^1,2,3,4, DUAN Liqin^1,2,3,4, WANG Qidong^1,4

¹CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, Shandong, China;
²Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, Shandong, China;
³University of Chinese Academy of Sciences, Beijing 100049, China;
⁴Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, Shandong, China

Received:2023-07-03 Revised:2023-09-21 Online:2023-11-15 Published:2024-05-15

摘要/Abstract

摘要： 氟喹诺酮类抗生素(FQs)是使用量最大的抗生素之一,是一种在环境中具有“伪持久性”的新型污染物,具有巨大的生态风险。FQs会改变微生物群落结构和功能从而影响微生物介导的氮循环过程,改变环境中各种类型氮的组分,进而对全球氮循环产生重要影响。本文总结了FQs污染现状及对微生物介导氮循环的影响,以阐明FQs对氮循环各个关键过程的作用,从而为揭示FQs的生态效应研究提供科学依据。FQs普遍存在于各类环境介质中,不同环境中FQs浓度和种类存在显著差异,其中氧氟沙星、诺氟沙星、环丙沙星和恩诺沙星是检出频率和浓度最高的4种FQs。FQs对氮循环的作用过程具有典型的剂量和种类依赖型特征。FQs主要通过降低氨氧化过程相关的amoA基因丰度和氨氧化细菌丰度、组成来抑制硝化过程;主要通过降低相关酶活性和narG、nirS、norB和nosZ等基因丰度,以及降低反硝化功能属微生物丰度、组成来抑制反硝化过程;通过降低厌氧氨氧化细菌丰度、组成和hzo基因丰度来限制厌氧氨氧化过程;最终导致环境中活性氮去除的降低和氧化亚氮(N₂O)释放的增加,甚至进一步诱发水体富营养化和温室效应等环境问题。未来应重点关注低浓度FQs和复合抗生素对氮循环过程的影响,进一步加强FQs对氮循环微生物单体和群落变化影响的研究。

关键词: 氟喹诺酮类抗生素(FQs), 微生物, 氮循环, 抑制作用, 功能基因

Abstract: Fluoroquinolone antibiotics (FQs) are one of the most widely used antibiotics, which are new pollutants with ‘pseudo persistence’ in the environment, causing great ecological risks. FQs could change the structure and function of microbial communities and affect nitrogen cycling mediated by microorganisms. Consequently, FQs would change the composition of various types of nitrogen in the environment and exert a significant impact on the global nitrogen cycling. We encapsulated the distribution of FQs in the environment and its impacts on nitrogen cycling mediated by microorganisms, explained the role of FQs in each key process of nitrogen cycling, aiming to provide an important reference for revealing the ecological effects of FQs. Generally, FQs could be detected in various environmental media, with significant differences in the concentration and types of FQs in different environments. Ofloxacin, norfloxacin, ciprofloxacin, and enrofloxacin are the four types of FQs with the highest detection frequency and concentration. The effect of FQs on nitrogen cycling deeply depends on typical characteristics of concentration and species. FQs mainly inhibit nitrification by reducing the abundance of amoA gene related to ammoxidation process and the abundance and composition of ammoxidation bacteria. FQs inhibits nitrification by reducing the abundance and composition of microbial communities. The denitrification process is mainly inhibited due to the reduction of the activity of related enzymes and the abundance of genes such as narG, nirS, norB, and nosZ genes, as well as the abundance and composition of denitrifying functional microorganisms. The process of anammox is restricted due to the reduction of the abundance, composition and hzo gene abundance of anaerobic anammox bacteria. FQs lead to the reduction of active nitrogen removal and the increase of N₂O release in the environment, with further environmental problems such as water eutrophication and greenhouse effect. In the future, we should pay attention to the effects of low concentration FQs and complex antibiotics on the nitrogen cycling, and focus on the effects of FQs on the changes of nitrogen cycle-related microbial monomers and communities.

Key words: fluoroquinolones antibiotics (FQs), microorganism, nitrogen cycle, inhibition, functional gene

温丽联, 宋金明, 李学刚, 马骏, 戴佳佳, 袁华茂, 段丽琴, 王启栋. 氟喹诺酮类抗生素的环境污染及其对微生物介导氮循环的影响[J]. 应用生态学报, 2023, 34(11): 3114-3126.

WEN Lilian, SONG Jinming, LI Xuegang, MA Jun, DAI Jiajia, YUAN Huamao, DUAN Liqin, WANG Qidong. Environmental pollution of fluoroquinolones and its relationship with nitrogen cycling mediated by microorganisms.[J]. Chinese Journal of Applied Ecology, 2023, 34(11): 3114-3126.

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氟喹诺酮类抗生素的环境污染及其对微生物介导氮循环的影响

Environmental pollution of fluoroquinolones and its relationship with nitrogen cycling mediated by microorganisms.

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