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• 研究简报 • 上一篇    

微囊藻毒素与饮用水消毒副产物复合诱导草鱼淋巴细胞凋亡

倪婉敏1;张杭君1,2;张建英1;姚超英3   

  1. 1浙江大学环境与资源学院生态环境研究中心, 杭州 310028;2杭州师范大学生命与环境科学学院, 杭州 310036; 3杭州市职业技术学院, 杭州 310004
  • 收稿日期:2008-06-02 修回日期:1900-01-01 出版日期:2009-01-20 发布日期:2009-01-20

Apoptosis of grass carp (Ctenopharngodon idellus) lymphocytes induced by the combination of microcystins and disinfection by-products in drinking water.

NI Wan-min1;ZHANG Hang-jun1,2;ZHANG Jian-ying1;YAO Chao-ying3   

  1. 1Environment and Ecology Research Center, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310028, China;2College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China;3Hangzhou Vocational and Technical College, Hangzhou 310004, China
  • Received:2008-06-02 Revised:1900-01-01 Online:2009-01-20 Published:2009-01-20

摘要: 针对饮用水中消毒副产物与微囊藻毒素的低剂量复合污染问题,采用离体细胞培养诱导方法,以草鱼淋巴细胞为暴露受体,研究了MCLR、MCRR两种微囊藻毒素和CHClBr2、CHCl2Br两种消毒副产物的单独与联合毒效应.结果表明:4种污染物在试验浓度下暴露2 h即能使草鱼淋巴细胞发生凋亡,且呈显著的剂量-效应关系;1 nmol·L-1MCLR、MCRR分别和1~100 nmol·L-1的CHCl2Br、CHClBr2的联合作用均表现为相加作用,并且均呈显著的剂量-效应关系.草鱼淋巴细胞凋亡率可作为一种有效的指标来评价微囊藻毒素及消毒副产物复合污染引起的细胞毒性.

关键词: 稳态转换, 替代稳态, 生态系统, 生态阈值, 环境管理

Abstract: Aiming at the combined pollution of low dose microcystins (MCs) and di sinfection by-products (DBPs) in drinking water, the individual and combined cy totoxic effects of two MCs (MCLR and MCRR) and two DBP compounds (CHClBr2 and CH Cl2Br) on the apoptosis of isolated grass carp (Ctenopharngodon idellus) lymphoc ytes were examined by using in vitro bioassays. The results showed that after 2 hours exposure to the four pollutants within the range of test concentrations, t he apoptosis of isolated lymphocyte occurred, and there existed significant dose -effect relationship. The combined cytotoxic effects of 1 nmol·L-1 MCLR or MCRR and 1〖KG-*2〗-〖KG-*7〗100 nmol·L-1 CHClBr2 or CHCl2Br were all of additive, and the apoptosis percent was highly correlated with the exposure dose. It was suggested that the apoptosis percent of grass carp lymphocytes could be used as an effective indicator to evaluate the cytotoxicity caused by the co mbined pollution of MCs and DBPs.

Key words: ecosystem, environmental management., alternative stable state, ecological threshold, regime shift