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应用生态学报 ›› 2009, Vol. 20 ›› Issue (08): 2032-2037.

• 研究报告 • 上一篇    下一篇

镉胁迫对平邑甜茶脂肪酸构成及脂质过氧化的影响

由淑贞;杨洪强;张龙;邵小杰   

  1. 山东农业大学园艺科学与工程学院作物生物学国家重点实验室| 山东泰安 271018
  • 收稿日期:2009-01-04 出版日期:2009-08-20 发布日期:2009-08-20

Effects of cadmium stress on fatty acid composition and lipid peroxidation of Malus hupehensis.

YOU Shu-zhen;YANG Hong-qiang;ZHANG Long;SHAO Xiao-jie   

  1. State Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271018, Shandong, China
  • Received:2009-01-04 Online:2009-08-20 Published:2009-08-20

摘要: 以平邑甜茶幼苗为试材,研究了镉胁迫下幼苗叶片和根系膜脂肪酸构成、活性氧、脂氧合酶和丙二醛含量的变化.结果表明:氯化镉处理后7~12 h,脂肪酸种类及其相对含量变化最为明显.处理后7 h,叶片和根系脂肪酸不饱和水平升至最高,含量分别达8282%和7243%;叶片可检测到的脂肪酸在处理后12 h由11种增至14种,根系则在处理17 h后由4种增至6种.O2.-产生速率在处理3 h、H2O2含量在处理7 h时升至最高,丙二醛含量和脂氧合酶活性则随着处理时间的延长逐渐增加.镉胁迫通过诱导活性氧和脂氧合酶来改变平邑甜茶脂肪酸构成,并引起脂质过氧化;镉处理12 h前,脂质过氧化是活性氧和脂氧合酶的共同结果;但处理12 h后,脂质过氧化加剧主要在于脂氧合酶活性的持续增加.

关键词: 镉, 平邑甜茶, 脂肪酸, 脂氧合酶, 黄土高原, 半干旱区, 苹果园地, 密度, 水分生产力, WinEPIC模型

Abstract: This paper studied the fatty acid composition, reactive oxygen species (ROS), lipoxygenase (LOX) activity, and malondialdehyde (MDA) content in the leaves and roots of Malus hupehensis seedlings under effects of cadmium (Cd) stress. Noticeable changes were observed in the kinds and relative contents of fatty acids after treated with CdCl2 for 7〖KG-*2〗-〖KG-*7〗12 hours. The relative contents of unsaturated fatty acids in leaves and roots reached the maximum after treated for 7 hours, being 8282% and 7243%, respectively. The kinds of fatty acids in leaves increased from 11 to 14 after treated for 12 hours, while those in roots increased from 4 to 6 after treated for 17 hours. The O2〖SX(B-*3〗-〖〗·〖SX)〗 generation rate and the H2O2 content reached the maximum after treated for 3 and 7 hours, respectively, and the MDA content and LOX activity increased with treating time. Cd stress altered the fatty acid composition of
Malus hupehensis via the inducement of reactive oxygen species and lipoxygenase, and induced lipid peroxidation, which was caused by both ROS and LOX within the first 12 hours of CdCl2 treatment and mainly by the increase of LOX activity since then.

Key words: cadmium, Malus hupehensis, fatty acid, lipoxygenase, Loess Plateau, semi-arid region, apple orchard, planting density, water productivity, WinEPIC model.