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土壤碳水化合物的测定方法及其指示作用

张威1,2;解宏图1;何红波1;郑立臣1,2;王鸽3   

  1. 1中国科学院沈阳应用生态研究所陆地生态过程重点实验室, 沈阳 110016;2中国科学院研究生院, 北京 100039;3沈阳农业大学, 沈阳 110161
  • 收稿日期:2005-11-08 修回日期:2006-06-16 出版日期:2006-08-18 发布日期:2006-08-18

Soil carbohydrates: their determination methods and indication functions

ZHANG Wei1,2; XIE Hongtu1; HE Hongbo1; ZHENG Lichen1,2; Wang Ge3   

  1. 1Key Laboratory of Terrestrial Ecological Process, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China; 2Graduate University of Chinese Academy of Sciences, Beijing 100039, China; 3Shenyang Agricultural University, Shenyang 110161, China
  • Received:2005-11-08 Revised:2006-06-16 Online:2006-08-18 Published:2006-08-18

摘要: 碳水化合物是土壤有机质的重要组成成分,也是土壤中易降解的有机成分之一,对土壤有机质的转化和土壤团聚体的形成有着重要影响.土壤碳水化合物的水解方法主要有硫酸、盐酸和三氟乙酸水解法,其检测方法主要有比色分析法、气相色谱法、液相色谱法和高效阴离子交换色谱脉冲电流检测法.文中论述了土壤碳水化合物的水解、纯化与检测方法,并重点介绍了气相色谱的衍生方法及各方法的优缺点,简要概述了碳水化合物对土壤有机质变化的指示作用.

关键词: 土壤水分亏缺, 生态反应, 生物量, 阔叶红松林

Abstract: Soil carbohydrates are the important component of soil organic matter, and play an important role in soil aggregation formation. Their hydrolysis methods involve sulfur acid (H2SO4), hydrochloric acid (HCl), and trifluoroacetic acid (TFA) hydrolysis, and their determination methods include colorimetry, gasliquid chromatography (GLC), high performance liquid chromatography (HPLC), and high performance anionexchange chromatography with pulsed amperometric detection (HPAE-PAD). This paper summarized the methods of carbohydrates’ hydrolysis, purification and detection, with focus on the derived methods of GLC, and briefly introduced the indication functions of carbohydrates in soil organic matter turnover.

Key words: Soil water stress, Ecological response, Biomass, Pine broadleaf forest