土壤氢肥效应的微生物学机制

doi:10.13287/j.1001-9332.202409.012

摘要/Abstract

摘要： 长期以来,豆科与非豆科作物间作、轮作因可以在减少氮肥施用的同时增加作物产量,是农田生态系统普遍采用的一项重要管理措施。当前,对这一管理方式减肥增效的解释主要集中在豆科对非豆科植物氮(N)磷(P)等养分供应的提高、土壤空间异质性增大导致的养分空间利用效率的提高以及土壤结构和抗病性的改善等方面。然而,这些现有理论还无法完全解释豆科与非豆科作物间作、轮作的增产效益。豆科植物根瘤固氮过程会释放大量副产物氢气(H₂),这些H₂可在豆科植物根瘤附近土壤中大量富集,但人们却很少在其土壤表面监测到H₂的流失。研究发现,根瘤固氮释放的H₂可被氢氧化细菌(HOB)迅速吸收利用(以H₂作为电子供体通过氧化还原反应同化固定CO₂合成细胞物质)并对植物生长产生促进作用。然而,人们至今对这种“氢肥效应”产生的生物学机理和生态学过程仍不清楚。本文综述了国内外关于H₂诱导的植物促生效应及其产生的微生物学机理,旨在开辟农田生态系统生产力提升新途径,为H₂在农业生产中的应用提供科学依据。

关键词: 氢气, 土壤微生物, 氢氧化细菌, 根际促生菌, 土壤过程

Abstract: For a long time, intercropping and rotation of leguminous with non-leguminous crops is widely used to reduce the application of nitrogen fertilizer and increase yield in agroecosystems. At present, most researchers considered that this management measure is helpful for reducing fertilizer consumption and increasing its efficiency, as it can improve nutrient supply of legumestonon-legumes, the spatial nutrient utilization efficiency by enhancing soil spatial heterogeneity, and improve soil structure and disease resistance. However, current theories cannot fully explain the positive effect of crop rotation and inter-cropping systems involving legumes. A large amount of hydrogen (H₂) can be produced as an obligatory by-product of nitrogenase responsible for nitrogen (N₂) fixation in the root nodules of leguminous plants. Despite of substantial amounts of H₂ enriched in the rhizosphere of legumes, only a minor proportion of H₂ is found to leak to soil surface. Increasing evidence showed that most H₂ released in soil is immediately depleted in the surrounding of N₂-fixing nodules by H₂-oxidizing bacteria (HOB) thriving in soil. HOB can use H₂ as an electron donor to assimilate and fix CO₂ through redox reactions to synthesize cellular substances and consequently promote plant growth. To date, however, little is known about the biological mechanism and ecological process behind the “hydrogen fertilizer effect”. Therefore, we review the H₂-induced plant growth-promoting effects and its microbiological mechanisms. Our aims were to explore a new way for enhancing agroecosystem production, and to provide scientific basis for future utilization of H₂ in agricultural production practices.

Key words: hydrogen, soil microorganism, H₂-oxidizing bacteria, plant growth-promoting rhizobacteria, soil processes

冯清山, 毛勤江, 马建国, 李玉满, 杨小倩, 鲁星鑫, 王晓波. 土壤氢肥效应的微生物学机制[J]. 应用生态学报, 2024, 35(9): 2382-2391.

FENG Qingshan, MAO Qinjiang, MA Jianguo, LI Yuman, YANG Xiaoqian, LU Xingxin, WANG Xiaobo. Microbiological mechanism of hydrogen fertilizer effect in soil[J]. Chinese Journal of Applied Ecology, 2024, 35(9): 2382-2391.

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