长白山赤杨丛枝菌根真菌多样性的半巢式LP-PCR-SSCP检测分析

摘要/Abstract

摘要： 利用半巢式LPPCRSSCP技术,对中国吉林长白山不同海拔生境下3种赤杨共生丛枝菌根真菌的多样性进行检测分析.结果表明,该地区赤杨属东北赤杨、西伯利亚赤杨及色赤杨共生丛枝菌根真菌在科乃至种的水平上并未随宿主的变化表现出丰富的多样性;3个树种在自身属的水平上与共生的球囊霉科(Glomaceae)至少1种AMF,即G.intraradix,在种的水平上表现出不相关于宿主海拔高度的某种相互选择性.

关键词: 赤杨, 丛枝菌根真菌, 半巢式LP-PCR-SSCP

Abstract: The diversity of arbuscular endomycorrhizal fungi colonized on three species of Alnus at Changbai Mountain was identified based on coupling the sensitivity of semi-nested LP-PCR and the specificity afforded by Single Strand Conformation Polymorphism analysis. It is suggested that altitudes and species of Alnus were not the important factors deciding the distribution of AMF in the grade of family, and there was at least one species of Glomaceae, Glomus intraradix, dominated in Alnus at Changbai Mountain.

Key words: Alnus, Arbuscular endomycorrhizal fungi, Semi-nested LP-PCR-SSCP

中图分类号:

Q949.32

李一叶, 何兴元, 张忠泽, 张成刚. 长白山赤杨丛枝菌根真菌多样性的半巢式LP-PCR-SSCP检测分析[J]. 应用生态学报, 2003, (11): 1837-1841.

LI Yiye, HE Xingyuan, ZHANG Zhongze, ZHANG Chenggang . Semi-nested LP-PCR-SSCP identification of arbuscular endomycorrhizal fungi diversity of Alnus at Changbai Mountain[J]. Chinese Journal of Applied Ecology, 2003, (11): 1837-1841.

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