氮添加对草地生态系统土壤pH、磷含量和磷酸酶活性的影响

doi:10.13287/j.1001-9332.202009.034

应用生态学报 ›› 2020, Vol. 31 ›› Issue (9): 2985-2992.doi: 10.13287/j.1001-9332.202009.034

氮添加对草地生态系统土壤pH、磷含量和磷酸酶活性的影响

田沐雨¹, 于春甲², 汪景宽^1*, 丁凡¹, 陈振华³, 姜楠³, 蒋晖⁴, 陈利军³

¹沈阳农业大学土地与环境学院, 沈阳 110866;
²辽宁大学环境学院, 沈阳 110036;
³中国科学院沈阳应用生态研究所, 沈阳 110016;
⁴大连市检验检测认证技术服务中心, 辽宁大连 116037

收稿日期:2019-12-16 接受日期:2020-05-28 出版日期:2020-09-15 发布日期:2021-03-15
通讯作者: * E-mail: j-kwang@163.com
作者简介:田沐雨, 女, 1993年生, 硕士研究生。主要从事草地土壤磷的研究。E-mail: tian_muyu@163.com
基金资助:
国家自然科学基金项目(41877108)和国家重点研发专项(2016YFD0300802)资助

Effect of nitrogen additions on soil pH, phosphorus contents and phosphatase activities in grassland

TIAN Mu-yu¹, YU Chun-jia², WANG Jing-kuan^1*, DING Fan¹, CHEN Zhen-hua³, JIANG Nan³, JIANG Hui⁴, CHEN Li-jun³

¹College of Land and Environment, Shenyang Agricultural University, Shenyang 110866, China;
²School of Environment, Liaoning University, Shenyang 110036, China;
³Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China;
⁴Dalian Center for Certification and Food and Drug Control, Dalian 116037, Liaoning, China

Received:2019-12-16 Accepted:2020-05-28 Online:2020-09-15 Published:2021-03-15
Contact: * E-mail: j-kwang@163.com
Supported by:
the National Natural Science Foundation of China (41877108) and the National Key Research and Development Program of China (2016YFD0300802)

摘要/Abstract

摘要： 磷是植物必需的大量营养元素之一,也是草地生态系统功能的重要限制因子。近年来,随着全球氮沉降的迅速增加,草地生态系统土壤磷及磷酸酶活性受到不同程度的影响。本研究采用整合分析方法,分析了草地的氮添加量、氮源种类、持续时间和土层深度等对土壤pH、全磷(TP)含量、有效磷(AP)含量、碱性磷酸酶(AlP)和酸性磷酸酶(AcP)活性的影响,以及土壤pH与磷酸酶活性的相关性。结果表明: 氮添加降低了土壤pH、TP含量和AlP活性,提高了土壤AcP活性,但对土壤AP含量无显著影响。从氮添加量来看,土壤pH、AlP显著降低在氮添加>5 g·m^-2·a^-1条件下即可发生;高水平氮添加(>10 g·m^-2·a^-1)导致AcP活性显著提高;土壤TP、AP含量显著降低仅发生在氮添加量为5～10 g·m^-2·a^-1条件下。硝酸铵处理显著降低了土壤TP含量,提高了AcP活性;尿素处理显著降低了土壤pH和AlP活性。在所有添加量下,当试验持续时间为3～10年时,土壤TP含量、AlP活性显著降低;持续时间大于3年时,土壤pH显著降低;>10年时,AcP活性显著提高。0～10 cm土层的AP含量显著升高,TP含量和AlP活性显著降低;大于10 cm土层中AP含量显著降低。土壤pH与土壤AcP活性呈显著负相关,表明氮添加引起的土壤pH改变可能是土壤磷酸酶活性变化的重要原因。

关键词: 草地, 氮添加, 土壤pH, 土壤磷, 土壤磷酸酶, 整合分析

Abstract: Phosphorus is a key nutrient for all plant species and a limiting factor for grassland ecosystem function. In recent years, in response to the rapid increase of global nitrogen deposition, soil phosphorus contents and phosphatase activities changed to varying degrees in grassland ecosystems. We conducted a meta-analysis to examine the responses of soil pH, total phosphorus (TP), available phosphorus (AP), as well as activities of alkaline phosphatase (AlP) and acid phosphatase (AcP) in soils to nitrogen addition amount, nitrogen type, experimental duration, and sampling depth. The correlation between soil pH and phosphatase response ratio was investigated. The results showed that nitrogen addition significantly reduced soil pH, TP and AlP activity, while significantly increased AcP activity, but had no significant effect on AP. Soil pH and AlP activity significantly decreased under nitrogen addition >5 g·m^-2·a^-1, and AcP activity significantly increased under high nitrogen addition (>10 g·m^-2·a^-1). The contents of TP and AP significantly decreased when nitrogen addition was 5-10 g·m^-2·a^-1. NH₄NO₃ treatment significantly reduced soil TP and increased AcP activity, while urea treatment significantly reduced soil pH and AlP activity. Across all nitrogen addition amounts, when the experiment duration was 3 to 10 years, soil TP content and AlP activity were significantly reduced. Soil pH was significantly reduced after three years nitrogen addition, and AcP activitiy was significantly increased after 10 years nitrogen addition. In the 0-10 cm soil layer, the TP content and AlP activity significantly decreased, while the AP content significantly increased. In >10 cm soil layer, the AP content was significantly decreased. The significant negative correlation between soil pH and AcP activity indicated that change in soil pH caused by nitrogen addition may be an important factor for the variation of soil phosphatase activity.

Key words: grassland, nitrogen addition, pH, phosphorus, phosphatase, meta-analysis

田沐雨, 于春甲, 汪景宽, 丁凡, 陈振华, 姜楠, 蒋晖, 陈利军. 氮添加对草地生态系统土壤pH、磷含量和磷酸酶活性的影响[J]. 应用生态学报, 2020, 31(9): 2985-2992.

TIAN Mu-yu, YU Chun-jia, WANG Jing-kuan, DING Fan, CHEN Zhen-hua, JIANG Nan, JIANG Hui, CHEN Li-jun. Effect of nitrogen additions on soil pH, phosphorus contents and phosphatase activities in grassland[J]. Chinese Journal of Applied Ecology, 2020, 31(9): 2985-2992.

参考文献

[1] 黄巧云, 林启美, 徐建明. 土壤生物化学. 北京: 高等教育出版社, 2015: 261-269 [Huang Q-Y, Lin Q-M, Xu J-M. Soil Biochemistry. Beijing: Higher Education Press, 2015: 261-269]
[2] 赵琼, 曾德慧. 陆地生态系统磷素循环及其影响因素. 植物生态学报, 2005, 29(1): 157-167 [Zhao Q, Zeng D-H. Phosphorus cycling in terrestrial ecosystems and its controlling factors. Chinese Journal of Plant Eco-logy, 2005, 29(1): 157-167]
[3] Wardle DA, Gundale MJ, Jäderlund A, et al. Decoupled long-term effects of nutrient enrichment on aboveground and belowground properties in subalpine tundra. Ecology, 2013, 94: 904-919
[4] Tian D, Niu S. A global analysis of soil acidification caused by nitrogen addition. Environmental Research Letters, 2015, 10: doi: 10.1088/1748-9326/10/2/024019
[5] Fang Y, Xun F, Bai W, et al. Long-term nitrogen addition leads to loss of species richness due to litter accumulation and soil acidification in a temperate steppe. Plos One, 2012, 7(10): e47369, doi: 10.1371/journal.pone.0047369
[6] Gong S, Zhang T, Guo R, et al. Response of soil enzyme activity to warming and nitrogen addition in a meadow steppe. Soil Research, 2015, 53: 242-252
[7] 田纪辉. 水分/养分添加对不同利用历史草地土壤氮、磷组分及酶促周转的影响. 博士论文. 北京: 中国科学院大学, 2017 [Tian J-H. Effects of Water and Nutrient Additions on Soil Nitrogen and Phosphorus Fractions and their Enzymatic Turnover in Grasslands with Different Land Use History. PhD Thesis. Beijing: University of Chinese Academy of Sciences, 2017]
[8] Zhang GN, Chen ZH, Zhang AM, et al. Nitrogen and phosphorus related hydrolytic enzyme activities influenced by N deposition under semi-arid grassland soil. Advanced Materials Research, 2013, 726-731: 3847-3854
[9] 彭少麟, 郑凤英. Meta分析及MetaWin软件. 土壤与环境, 1999, 8(4): 295-299 [Peng S-L, Zheng F-Y. Introduction of Meta Win Software. Soil and Environmental Sciences, 1999, 8(4): 295-299]
[10] 李焕茹. 碳氮调节对草地土壤元素含量、酶活性及植物生长的影响. 硕士论文. 北京: 中国科学院大学, 2018 [Li H-R. Effects of Carbon and Nitrogen Additions on Soil Element Contents, Enzyme Activities and Plant Growth in Grasslands. Master Thesis. Beijing: University of Chinese Academy of Sciences, 2018]
[11] 朱莹. 氮沉降对呼伦贝尔草地土壤生物学特性的影响. 硕士论文. 北京: 中国科学院大学, 2018 [Zhu Y. Effects of Nitrogen Deposition on Soil Biological Characteristics in Hulun Buir Grassland. Master Thesis. Beijing: University of Chinese Academy of Sciences, 2018]
[12] 赵晓琛, 皇甫超河, 刘红梅, 等. 贝加尔针茅草原土壤酶活性及微生物量碳氮对养分添加的响应. 草地学报, 2016, 24(1): 47-53 [Zhao X-C, Huangfu C-H, Liu H-M, et al. Response of soil enzyme activity and microbial biomass carbon and nitrogen to the nutrient addition of Stipa baicalensis steppe in Inner Mongolia. Acta Agrestia Sinica, 2016, 24(1): 47-53]
[13] Wang RZ, Filley TR, Xu Z, et al. Coupled response of soil carbon and nitrogen pools and enzyme activities to nitrogen and water addition in a semi-arid grassland of Inner Mongolia. Plant and Soil, 2014, 381: 323-336
[14] Kritzler UH, Johnson D. Mineralisation of carbon and plant uptake of phosphorus from microbially-derived organic matter in response to 19 years simulated nitrogen deposition. Plant and Soil, 2010, 326: 311-319
[15] Zhang NY, Guo R, Song P, et al. Effects of warming and nitrogen deposition on the coupling mechanism between soil nitrogen and phosphorus in Songnen Meadow Steppe, northeastern China. Soil Biology and Biochemistry, 2013, 65: 96-104
[16] Long M, Wu HH, Smith MD, et al. Nitrogen deposition promotes phosphorus uptake of plants in a semi-arid temperate grassland. Plant and Soil, 2016, 408: 475-484
[17] 陈继辉, 李炎朋, 熊雪, 等. 磷添加对草地土壤酸度和化学计量学特征的影响. 草业科学, 2017, 34(5): 943-949 [Chen J-H, Li Y-P, Xiong X, et al. Effect of nitrogen and phosphorus addition on soil acidity and stoichiometry characteristics in a typical temperate grassland in Inner Mongolia. Pratacultural Science, 2017, 34(5): 943-949]
[18] 魏金明, 姜勇, 符明明, 等. 水、肥添加对内蒙古典型草原土壤碳、氮、磷及pH的影响. 生态学杂志, 2011, 30(8): 1642-1646 [Wei J-M, Jiang Y, Fu M-M, et al. Effects of water addition and fertilization on soil nutrient contents and pH value of typical grassland in Inner Mongolia. Chinese Journal of Ecology, 2011, 30(8): 1642-1646]
[19] 张宇. 荒漠草原土壤特性对增温和施氮的响应. 硕士论文. 呼和浩特: 内蒙古农业大学, 2014 [Zhang Y. The Response of Soil Properties to Experimental Warming and Nitrogen Addition in Desert Steppe. Master Thesis. Huhhot: Inner Mongolia Agricultural University, 2014]
[20] 孙亚男, 李茜, 李以康, 等. 氮、磷养分添加对高寒草甸土壤酶活性的影响. 草业学报, 2016, 25(2): 18-26 [Sun Y-N, Li Q, Li Y-K, et al. The effect of nitrogen and phosphorus applications on soil enzyme activities in Qinghai-Tibetan alpine meadows. Acta Prataculturae Sinica, 2016, 25(2): 18-26]
[21] Li LJ, Zeng DH, Mao R, et al. Nitrogen and phosphorus resorption of Artemisia scoparia, Chenopodium acuminatum, Cannabis sativa, and Phragmites communis under nitrogen and phosphorus additions in a semiarid grassland, China. Plant, Soil and Environment, 2012, 58: 446-451
[22] 杨山. 水肥添加对内蒙古典型草原土壤微生物学特性的影响. 硕士论文. 沈阳: 沈阳大学, 2014 [Yang S. Effects of Water and Fertilizer Addition on Soil Microbiological Characteristics in a Typical Grassland of Inner Mongolia. Master Thesis. Shenyang: Shenyang University, 2014]
[23] 郭红玉. 模拟增温和氮素添加对高寒草甸草地的影响. 硕士论文. 西宁: 青海大学, 2015 [Guo H-Y. The Impact of the Simulative Warming and Adding Nitrogen on Grassland in Alpine Meadow. Master Thesis. Xining: Qinghai University, 2015]
[24] Wang HY, Wen WZ, Ding R, et al. The impacts of nitrogen deposition on community N:P stoichiometry do not depend on phosphorus availability in a temperate meadow steppe. Environmental Pollution, 2018, 242: 82-89
[25] Luo R, Fan J, Wang W, et al. Nitrogen and phosphorus enrichment accelerates soil organic carbon loss in alpine grassland on the Qinghai-Tibetan Plateau. Science of the Total Environment, 2019, 650: 303-312
[26] Liu J, Zhang X, Wang H, et al. Long-term nitrogen fertilization impacts soil fungal and bacterial community structures in a dryland soil of Loess Plateau in China. Journal of Soils and Sediments, 2018, 18: 1632-1640
[27] 周纪东, 史荣久, 赵峰, 等. 施氮频率和强度对内蒙古温带草原土壤pH及碳、氮、磷含量的影响. 应用生态学报, 2016, 27(8): 2467-2476 [Zhou J-D, Shi R-J, Zhao F, et al. Effects of the frequency and intensity of nitrogen addition on soil pH, the contents of carbon, nitrogen and phosphorus in temperate steppe in Inner Mongolia, China. Chinese Journal of Applied Ecology, 2016, 27(8): 2467-2476]
[28] Stursova M, Crenshaw CL, Sinsabaugh RL. Microbial responses to long-term n deposition in a semiarid grassland. Microbial Ecology, 2006, 51: 90-98
[29] Zeglin LH, Collins SM. Microbial responses to nitrogen addition in three contrasting grassland ecosystems. Oecologia, 2007, 154: 349-359
[30] Johnson DW, Leake JR, Lee JA, et al. Changes in soil microbial biomass and microbial activities in response to 7 years simulated pollutant nitrogen deposition on a heathland and two grasslands. Environmental Pollution, 1998, 103: 239-250
[31] Chen S, Zhang S, Yan Z, et al. Differences in main processes to transform phosphorus influenced by ammonium nitrogen in flooded intensive agricultural and steppe soils. Chemosphere, 2019, 226: 192-200
[32] Zhang Y, Wang C, Li Y. Contrasting effects of nitrogen and phosphorus additions on soil nitrous oxide fluxes and enzyme activities in an alpine wetland of the Tibetan Plateau. Plos One, 2019, 14(8): e216244, doi: 10.1371/journal.pone.0216244
[33] Tian XF, Hu HW, Ding Q, et al. Influence of nitrogen fertilization on soil ammonia oxidizer and denitrifier abundance, microbial biomass, and enzyme activities in an alpine meadow. Biology and Fertility of Soils, 2014, 50: 703-713
[34] Johnson DW, Leake JR, Read D, et al. Liming and nitrogen fertilization affects phosphatase activities, microbial biomass and mycorrhizal colonisation in upland grassland. Plant and Soil, 2005, 271: 157-164
[35] Li Y, Nie C, Liu Y, et al. Soil microbial community composition closely associates with specific enzyme activities and soil carbon chemistry in a long-term nitrogen fertilized grassland. Science of the Total Environment, 2019, 654: 264-274
[36] Shi Y, Sheng L, Wang Z, et al. Responses of soil enzyme activity and microbial community compositions to nitrogen addition in bulk and microaggregate soil in the temperate steppe of Inner Mongolia. Eurasian Soil Science, 2016, 49: 1149-1160
[37] Tao Z, Shaobo Y, Rui G, et al. Warming and nitrogen addition alter photosynthetic pigments, sugars and nutrients in a temperate meadow ecosystem. Plos One, 2016, 11(6): e155375, doi: 10.1371/journal.pone.0155375
[38] Li LJ, Zeng DH, Yu ZY, et al. Soil microbial properties under N and P additions in a semi-arid, sandy grassland. Biology and Fertility of Soils, 2010, 46: 653-658
[39] Messiga AJ, Ziadi N, Belanger G, et al. Relationship between soil phosphorus and phosphorus budget in grass sward with varying nitrogen applications. Soil Science Society of America Journal, 2014, 78: 1481-1488
[40] Dillard SL, Wood CW, Wood BH, et al. Effects of nitrogen fertilization on soil nutrient concentration and phosphatase activity and forage nutrient uptake from a grazed pasture system. Journal of Environmental Management, 2015, 154: 208-215
[41] Zhang N, Wan S, Li L, et al. Impacts of urea N addition on soil microbial community in a semi-arid tempe-rate steppe in northern China. Plant and Soil, 2008, 311: 19-28
[42] Wei C, Yu Q, Bai E, et al. Nitrogen deposition wea-kens plant-microbe interactions in grassland ecosystems. Global Change Biology, 2013, 19: 3688-3697
[43] Clegg CD. Impact of cattle grazing and inorganic fertiliser additions to managed grasslands on the microbial community composition of soils. Applied Soil Ecology, 2006, 31: 73-82
[44] 张海芳. 贝加尔针茅草原植物与土壤微生物群落对氮素和水分添加的响应. 博士论文. 北京: 中国农业科学院, 2017 [Zhang H-F. Responses of Plant and Soil Microbial Communities to Nitrogen and Water Addition on the Stipa baicalensis Steppe. PhD Thesis. Beijing: Chinese academy of Agricultural Sciences, 2017]
[45] Copeland SM, Bruna E, Silva LV, et al. Short-term effects of elevated precipitation and nitrogen on soil fertility and plant growth in a Neotropical savanna. Ecosphere, 2012, 3: 1-20
[46] 范珍珍, 王鑫, 王超, 等. 整合分析氮磷添加对土壤酶活性的影响. 应用生态学报, 2018, 29(4): 1266-1272 [Fan Z-Z, Wang X, Wang C, et al. Effect of nitrogen and phosphorus addition on soil enzyme activities: A meta-analysis. Chinses Journal of Applied Ecology, 2018, 29(4): 1266-1272]
[47] Curtis PS, Wang X. A meta-analysis of elevated CO₂ effects on woody plant mass, form, and physiology. Oecologia, 1998, 113: 299-313
[48] Horswill P, Odhran O, Phoenix GK, et al. Base cation depletion, eutrophication and acidification of species-rich grasslands in response to long-term simulated nitrogen deposition. Environmental Pollution, 2008, 155: 336-349
[49] Wang R, Dungait JA, Buss HL, et al. Base cations and micronutrients in soil aggregates as affected by enhanced nitrogen and water inputs in a semi-arid steppe grassland. Science of the Total Environment, 2017, 575: 564-572
[50] Jarvis SC, Stockdale EA, Shepherd MA, et al. Nitrogen mineralization in temperate agricultural soils: Processes and measurement. Advances in Agronomy, 1996, 57: 187-235
[51] 何利元, 郭群, 李胜功, 等. 氮磷添加对内蒙古温带草地地上生物量的影响.应用生态学报, 2015, 26(8): 2291-2297 [He L-Y, Guo Q, Li S-G, et al. Influence of nitrogen and phosphorus addition on the aboveground biomass in Inner Mongolia temperate steppe, China. Chinese Journal of Applied Ecology, 2015, 26(8): 2291-2297]
[52] Zhang YM, Wu N, Zhou GY, et al. Changes in enzyme activities of spruce (Picea balfouriana) forest soil as related to burning in the eastern Qinghai-Tibetan Plateau. Applied Soil Ecology, 2005, 30: 215-225
[53] Bloom AJ. Influence of inorganic nitrogen and pH on the elongation of maize seminal roots. Annals of Botany, 2006, 97: 867-873
[54] Watson C, Miller H, Poland P, et al. Soil properties and the ability of the urease inhibitor N-(n-BUTYL) thiophosphoric triamide (nBTPT) to reduce ammonia volatilization from surface-applied urea. Soil Biology and Biochemistry, 1994, 26: 1165-1171
[55] Spohn M, Kuzyakov Y. Phosphorus mineralization can be driven by microbial need for carbon. Soil Biology and Biochemistry, 2013, 61: 69-75
[56] 郑丽娜, 王先之, 沈禹颖. 保护性耕作对黄土高原塬区作物轮作系统磷动态的影响. 草业学报, 2011, 20(4): 19-26 [Zheng L-N, Wang X-Z, Shen Y-Y. Dynamic of phosphorus with wheat-soybean rotation systems under conservation tillage in the western Loess Plateau. Acta Prataculturae Sinica, 2011, 20(4): 19-26]
[57] Burns RG. Soil Enzyme. New York: Academic Press, 1978: 197-250
[58] Turner BL, Haygarth PM. Phosphatase activity in temperate pasture soils: Potential regulation of labile organic phosphorus turnover by phosphodiesterase activity. Science of the Total Environment, 2005, 344: 27-36
[59] Sakurai M, Wasaki J, Tomizawa Y, et al. Analysis of bacterial communities on alkaline phosphatase genes in soil supplied with organic matter. Soil Science and Plant Nutrition, 2008, 54: 62-71
[60] 刘宪斌. 模拟氮沉降和刈割对内蒙古典型草原土壤微生物量的影响. 中国农学通报, 2010, 26(13): 345-348 [Liu X-B. Effects of stimulated nitrogen disposition and cutting on soil microbial biomass in a typical steppe in Inner Mongolia. Chinese Agricultural Science Bulletin, 2010, 26(13): 345-348]
[61] Geisseler D, Lazicki PA, Scow KM, et al. Mineral nitrogen input decreases microbial biomass in soils under grasslands but not annual crops. Applied Soil Ecology, 2016, 106: 1-10
[62] 薄正熙, 游成铭, 胡中民, 等. 氮素与水分添加对内蒙古温带典型草原生物量的影响. 应用与环境生物学报, 2017, 23(4): 658-664 [Bo Z-X, You C-M, Hu Z-M, et al. The influence of nitrogen and water addition on biomass in a typical steppe of Inner Mongolia. Chinese Journal of Applied and Environmental Biology, 2017, 23(4): 658-664 ]
[63] 高宗宝, 王洪义, 吕晓涛, 等. 氮磷添加对呼伦贝尔草甸草原4种优势植物根系和叶片C∶N∶P化学计量特征的影响. 生态学杂志, 2017, 36(1):80-88 [Gao Z-B, Wang H-Y, Lyu H-T, et al. Effects of nitrogen and phosphorus addition on C:N:P stoichiometry in roots and leaves of four dominant plant species in a meadow steppe of Hulunbuir. Chinese Journal of Ecology, 2017, 36(1): 80-88]
[64] 冯玉龙, 孙国斌. 根系温度对植物的影响(Ⅰ):根温对植物生长及光合作用的影响. 东北林业大学学报, 1995, 23(3): 63-69 [Feng Y-L, Sun G-B. Influence of temperature of root system on plant (Ⅰ): Influence of root temperature on plant growth and photosynthesis. Journal of Northeast Forestry University, 1995, 23(3): 63-69]

氮添加对草地生态系统土壤pH、磷含量和磷酸酶活性的影响

Effect of nitrogen additions on soil pH, phosphorus contents and phosphatase activities in grassland

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	马建国, 李玉满, 王树林, 朱怀德, 姚梦凡, 王晓波. 典型黄帚橐吾型退化草地形成过程中土壤和植被特征 [J]. 应用生态学报, 2023, 34(8): 2153-2160.
[2]	孔东彦, 杨灵芳, 刁静文, 郭鹏. 不同生境下氮沉降对土壤N₂O通量影响的整合分析 [J]. 应用生态学报, 2023, 34(8): 2171-2177.
[3]	吴远秀, 刘婧桐, 丁聪, 张炳川, 梁潇洒, 宁宇, 殷江霞, 吕晓涛. 氮素输入和刈割对草甸草原植食性昆虫多度和物种丰富度的影响 [J]. 应用生态学报, 2023, 34(7): 1975-1980.
[4]	刘珊珊, 王全成, 史加勉, 刘子恺, 沈菊培, 贺纪正, 郑勇. 亚热带森林菌根植物根系真菌群落结构对氮磷添加的响应 [J]. 应用生态学报, 2023, 34(6): 1547-1554.
[5]	解晗, 李俊, 同小娟, 张静茹, 刘沛荣, 于裴洋, 胡海洋, 杨铭鑫. 2000—2018年黄河流域森林和草地物候的时空变化 [J]. 应用生态学报, 2023, 34(3): 647-656.
[6]	韦金菊, 秦国兵, 张庚金, 贾露露, 周建, 吴建富, 魏宗强. 不同粒径生物炭对土壤磷吸附-解吸特性的影响 [J]. 应用生态学报, 2023, 34(3): 708-716.
[7]	谷昌军, 张镱锂, 刘林山, 魏博, 宫殿清, 崔伯豪. 放牧压力表述的分异及其适用场景 [J]. 应用生态学报, 2023, 34(2): 433-441.
[8]	陈天, 程瑞梅, 沈雅飞, 肖文发, 王丽君, 孙鹏飞, 张萌, 李璟. 氮添加对三峡库区马尾松人工林土壤团聚体有机氮组分和氮矿化的影响 [J]. 应用生态学报, 2023, 34(10): 2601-2609.
[9]	肖向前, 张海阔, 冯娅斯, 王吉鹏, 梁辰飞, 陈有超, 朱高荻, 蔡延江. 植物残体对青藏高原高寒草甸土壤、微生物和胞外酶C∶N∶P化学计量特征的影响 [J]. 应用生态学报, 2023, 34(1): 58-66.
[10]	张晓晴, 曾泉鑫, 元晓春, 万晓华, 崔琚琰, 李文周, 林惠瑛, 谢欢, 陈文伟, 吴君梅, 陈岳民. 氮添加诱导的磷限制改变了亚热带黄山松林土壤微生物群落结构 [J]. 应用生态学报, 2023, 34(1): 203-212.
[11]	卢正宽, 刘贺永, 蹇述莲, 徐丽, 肖路, 王汝振, 姜勇, 张红香. 森林草原过渡带持久土壤种子库沿降水梯度的格局 [J]. 应用生态学报, 2022, 33(9): 2363-2370.
[12]	季飞龙, 李雪华, 李晓兰, 贾美玉, 赵志勇. 退化草地物种种内和种间功能性状变异对放牧的响应 [J]. 应用生态学报, 2022, 33(9): 2371-2378.
[13]	侯星辰, 鲁绍伟, 向昌林, 李少宁, 赵娜, 徐晓天. 不同母质温带草地植物群落多样性对人为干扰的响应 [J]. 应用生态学报, 2022, 33(8): 2153-2160.
[14]	曾泉鑫, 元晓春, 周嘉聪, 吴君梅, 李文周, 林惠瑛, 张晓晴, 陈岳民. 氮添加对毛竹林土壤酸性磷酸单酯酶动力学参数的影响 [J]. 应用生态学报, 2022, 33(8): 2178-2186.
[15]	宜树华, 陈世苹, 李英年, 胡中民. 中国生态脆弱区联网协同观测及其在承载力研究中的应用 [J]. 应用生态学报, 2022, 33(8): 2271-2278.