微生物分子生态学技术及其在环境污染研究中的应用

应用生态学报 ›› 2002, Vol. ›› Issue (2): 247-251.

微生物分子生态学技术及其在环境污染研究中的应用

钟鸣, 周启星

中国科学院沈阳应用生态研究所陆地生态过程开放研究实验室, 沈阳 110016

收稿日期:2001-08-17 修回日期:2001-10-23 出版日期:2002-02-15
通讯作者: 钟鸣,女,1971年生,博士,现在中国科学院沈阳应用生态研究所陆地生态过程开放研究实验室进行博士后工作,研究方向为分子生态学,发表论文6篇.E-mail:mingzh@mail.sy.ln.cn
基金资助:
中国科学院“引进国外杰出人才”及百人计划项目;中国科学院知识创新工程项目(KZCX401)

Molecular-ecological technology of microorganisms and its application to research on environmental pollution

ZHONG Ming, ZHOU Qixing

Open Laboratory of Terrestrial Ecological Process, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016

Received:2001-08-17 Revised:2001-10-23 Online:2002-02-15

摘要/Abstract

摘要： 较为系统地概述了核酸探针检测技术、利用引物的PCR技术、DNA序列分析技术和电泳分离及显示技术在国内外的研究进展,并探讨了这些技术在环境污染研究中的应用及其方向.结果表明,这些被认为是重要的微生物分子生态学技术,在探索微生物与污染环境之间的相互关系中发挥了重要作用,促进了污染环境的微生物遗传适应进化机制的研究、污染物的微生物降解有关基因的定位及微生物工程菌的构建等方面的工作,从而推进了污染环境微生物修复的分子生态学的发展.

关键词: 污染环境, 微生物, 污染物降解基因, 分子生态学

Abstract: Nucleicacid probe detection,PCRtechnique using primer,DNAsequential analysis,and electrophoresis separation and display were summarized and the application of these techniques to research on environmental pollution and future developing directions were discussed.It was pointed out that molecular-ecological technology of microorganisms are playing an important role in studying on relationships between microorganisms and contaminated environment.There are some important advances such as genetic adaptation and evolutionary mechanisms of microorganisms in contaminated environment,the positioning of pollutant-degrading genes of microorganisms and the construction of microbiological engineering bacteria,thus promoting the development of molecular ecology for bioremediation of contaminated environment.

Key words: Contaminated environment, Microorganism, Pollutant-degrading gene, Molecular ecology

中图分类号:

X172

钟鸣, 周启星. 微生物分子生态学技术及其在环境污染研究中的应用[J]. 应用生态学报, 2002, (2): 247-251.

ZHONG Ming, ZHOU Qixing . Molecular-ecological technology of microorganisms and its application to research on environmental pollution[J]. Chinese Journal of Applied Ecology, 2002, (2): 247-251.

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