毛乌素沙地中间锦鸡儿根瘤菌的多样性及其抗逆性

摘要/Abstract

摘要： 从内蒙古毛乌素沙地优势物种中间锦鸡儿收集根瘤,分离根瘤菌15株,对其进行同工酶电泳,聚类分析证明中间锦鸡儿的遗传多样性.对其耐盐性、耐酸碱性、生长温度范围、产酸产碱性、过氧化氢酶活性以及唯一碳源利用能力进行比较研究,结果表明,所有中间锦鸡儿根瘤菌均分泌H⁺,都具有过氧化氢酶活性,73.3%的菌株可以在含3%NaCl的YMA培养基上生长,80%的菌株可以抵抗50℃高温,除对乳糖和淀粉利用表现出差异外,中间锦鸡儿根瘤菌对其余碳源没有选择性,证明中间锦鸡儿根瘤菌具有较强的抗逆性,但仍存在菌株间差异,是对毛乌素沙地多种景观生态类型的适应.作为一种新的种质资源,中间锦鸡儿根瘤菌可用于恶劣生境固氮.

关键词: 中间锦鸡儿根瘤菌, 多样性, 抗逆性

Abstract: Fifteen rhizobia strains were isolated from wild shrubby legume Caragana intermedia in Maowusu sandland. A dendrogram was constructed based on esterase profiles, showing a rich diversity of these rhizobia. Many biochemical characteristics were detected, including acid or alkali production, catalase activity, utilization of sole carbon sources, and resistance to salt, acid-alkali and temperature variation. The results indicated that all the rhizobia strains isolated from Caragana intermedia could excrete H⁺ on YMA agar and produce catalase. 73.3% strains could tolerate NaCl stress at 3% concentration, and 80% strains could grow at 50℃.Except the difference in lactose and starch utilization, rhizobia strains had no bias on the rest carbon sources. However, the difference in resistance to stress existed among strains, which might be related to the adaptation of rhizobia to diverse landscapes in Maowusu sandland. It was revealed that rhizobia nodulating Caragana intermedia could be used as a new germplasm to fix nitrogen under severe environment.

Key words: Caragana intermedia Rhizobia, Diversity, Resistance

中图分类号:

高丽锋, 邓馨, 王洪新, 胡志昂. 毛乌素沙地中间锦鸡儿根瘤菌的多样性及其抗逆性[J]. 应用生态学报, 2004, (1): 44-48.

GAO Lifeng, DENG Xin, WANG Hongxin, HU Zhiang. Diversity and resistance of rhizobia isolated from Caragana intermedia in Maowusu sandland[J]. Chinese Journal of Applied Ecology, 2004, (1): 44-48.

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