外源褪黑素对高温胁迫下菊花光合和生理特性的影响

doi:10.13287/j.1001-9332.202107.025

应用生态学报 ›› 2021, Vol. 32 ›› Issue (7): 2496-2504.doi: 10.13287/j.1001-9332.202107.025

外源褪黑素对高温胁迫下菊花光合和生理特性的影响

齐晓媛, 王文莉^*, 胡少卿, 刘梦雨, 郑成淑, 孙宪芝

山东农业大学园艺科学与工程学院/作物生物学国家重点实验室, 山东泰安 271018

收稿日期:2020-10-30 修回日期:2021-04-25 出版日期:2021-07-15 发布日期:2022-01-15
通讯作者: *wangwenli169@163.com
作者简介:齐晓媛,女,1996年生,硕士研究生。主要从事观赏植物生理生态研究。E-mail:qixiaoyuan53@163.com
基金资助:
国家重点研发计划项目(2019YFD1001505)和山东省林业科技创新项目(LYCX06-2018-33)

Effects of exogenous melatonin on photosynthesis and physiological characteristics of chry-santhemum seedlings under high temperature stress

QI Xiao-yuan, WANG Wen-li^*, HU Shao-qing, LIU Meng-yu, ZHENG Cheng-shu, SUN Xian-zhi

College of Horticultural Science and Engineering, Shandong Agricultural University/State Key Laboratory of Crop Biology, Tai'an 271018, Shandong, China

Received:2020-10-30 Revised:2021-04-25 Online:2021-07-15 Published:2022-01-15
Contact: *wangwenli169@163.com
Supported by:
National Key R&D Program of China (2019YFD1001505) and the Forestry Science and Technology Innovation Project in Shandong Province (LYCX06-2018-33).

摘要/Abstract

摘要： 以切花菊品种‘神马'为试材,研究外源褪黑素(MT)对菊花抗高温胁迫的影响。将供试菊花叶面喷施200 μmol·L^-1的MT后,进行40 ℃(昼)/35 ℃(夜)高温胁迫,观察菊花叶片叶绿体和内囊体超微结构,测定光合和生理指标。结果表明:与常温对照(CK)相比,高温胁迫下菊花叶片叶绿体和类囊体受损,叶绿素含量和最大荧光(F_m)显著降低,OJIP曲线发生变化,K点和J点荧光升高,净光合速率(P_n)、蒸腾速率(T_r)、气孔导度(g_s)均显著降低,胞间CO₂浓度(C_i)均显著增加;相对电导率(REC)、丙二醛(MDA)、活性氧(ROS)、渗透调节物质含量和抗氧化酶活性显著增加。外源喷施MT可维持高温胁迫下植株叶绿体和类囊体结构的完整性,明显降低OJIP曲线中K点和J点上升的幅度,F_m、P_n、g_s、T_r和光合色素含量显著提高,C_i显著下降,缓解了高温胁迫对菊花光合和荧光作用的抑制。同时,外源喷施MT处理显著降低了高温胁迫下菊花REC、MDA和ROS含量,增强了菊花叶片中渗透调节物质含量和抗氧化酶活性。可见,外源MT可通过保护菊花叶片叶绿体结构的完整性,增强光合作用,抑制高温胁迫下菊花植株体内ROS的过度产生,提高抗氧化酶系统的活性,降低膜质过氧化水平和保护脂膜的完整性,从而提高菊花植株抗高温胁迫能力。

关键词: 菊花, 高温胁迫, 褪黑素, 光合, 生理机制

Abstract: We examined the effects of exogenous melatonin (MT) on the resistance of Chrysanthemum morifolium ‘Jinba' to high temperature stress. Chrysanthemum leaves were sprayed with 200 μmol·L^-1MT, and then subjected to high temperature stress at 40 ℃ (day)/ 35 ℃ (night). The ultrastructure of chloroplast and thylakoid of chrysanthemum leaves were observed, and the photosynthetic and physiological indices were measured. The results showed that the chloroplast and thyla-koid structures of chrysanthemum were damaged under high temperature stress. The chlorophyll contents and maximum fluorescence (F_m) were significantly reduced, while the OJIP curve changed with the fluorescence of K and J points increased. The net photosynthetic rate (P_n), transpiration rate (T_r) and stomatal conductance (g_s) were significantly decreased, while the internal CO₂ concentration (C_i) was significantly increased. The relative conductivity (REC), malondialdehyde (MDA), reactive oxygen species (ROS), osmotic adjustment substances content and antioxidant enzyme activity all increased significantly. Spraying exogenous MT onto leaves could maintain the integrity of chloroplast and thylakoid structure under high temperature in chrysanthemum and significantly decrease the increment in the K and J points of OJIP curve. Exogenous application of MT alleviated the inhibition of high temperature stress on photosynthesis and fluorescence of chrysanthemum, as indicated by significantly higher F_m, P_n, g_s, T_r and photosynthetic pigment contents and lower C_i. Exogenous MT also significantly reduced the REC, MDA and ROS contents of chrysanthemum under high temperature stress, and enhanced the osmotic adjustment substances content and antioxidant enzyme activity in chrysanthemum leaves. It suggested that exogenous MT could protect the integrity of chloroplast structure of chrysanthemum leaves, enhance photosynthesis, inhibit the excessive production of ROS in the plants under high temperature stress, improve the activity of antioxidant enzyme system, reduce the level of membrane peroxidation and keep the integrity of lipid membrane, and thus improve the ability of chrysanthemum plants to resist high temperature stress.

Key words: chrysanthemum, high temperature stress, melatonin, photosynthesis, physiologicalmechanism

齐晓媛, 王文莉, 胡少卿, 刘梦雨, 郑成淑, 孙宪芝. 外源褪黑素对高温胁迫下菊花光合和生理特性的影响[J]. 应用生态学报, 2021, 32(7): 2496-2504.

QI Xiao-yuan, WANG Wen-li, HU Shao-qing, LIU Meng-yu, ZHENG Cheng-shu, SUN Xian-zhi. Effects of exogenous melatonin on photosynthesis and physiological characteristics of chry-santhemum seedlings under high temperature stress[J]. Chinese Journal of Applied Ecology, 2021, 32(7): 2496-2504.

参考文献

[1] 解玉玲. 高温胁迫对植物生理影响的研究进展. 吉林农业, 2019(8): 107-108 [Xie Y-L. Research advances on influence of high temperature stress on some physiological characteristics of plants. Jilin Agriculture, 2019(8): 107-108]
[2] 徐暑晖. 外源褪黑素促进盐胁迫下玉米种子萌发的分子机理. 硕士论文. 扬州: 扬州大学, 2018 [Xu S-H. The Molecular Mechanism of Exogenous Melatonin in Promoting the Germination of Maize (Zea mays L.) Seeds under Salt Stress. Master Thesis. Yangzhou: Yang-zhou University, 2018]
[3] 陈亚娇. 切花菊特征特性及实用栽培技术. 中国农技推广, 2019, 35(6): 44 [Chen Y-J. Characteristics and practical cultivation techniques of cut chrysanthemum. China Agricultural Technology Extension, 2019, 35(6): 44]
[4] 刘易超. 高温胁迫对菊花茎叶电阻抗图谱参数及叶绿素荧光特性的影响. 硕士论文. 保定: 河北农业大学, 2012 [Liu Y-C. Effects of High Temperature Stress on Electrical Impedance Spectrum Parameters and Chlorophyll Fluorescence Characteristics of Chrysanthemum. Master Thesis. Baoding: Hebei Agricultural University, 2012]
[5] 刘易超, 肖建忠, 杨继双, 等. 高温胁迫对菊花叶片部分生理参数的影响. 河北农业大学学报, 2011, 34(6): 46-49 [Liu Y-C, Xiao J-Z, Yang J-S, et al. Effects of high temperature stress on physiological parameters of chrysanthemum. Journal of Hebei Agricultural University, 2011, 34(6): 46-49]
[6] 黄陈珏, 刘芳, 马琳琳, 等. 外源褪黑素对樱桃番茄果实品质的影响. 山西农业科学, 2020, 48(4): 527-530 [Huang C-Y, Liu F, Ma L-L, et al. Effect of exogenous melatonin on the quality of cherry tomato fruit. Journal of Shanxi Agricultural Sciences, 2020, 48(4): 527-530]
[7] Dubbels R, Reiter RJ, Klenke E, et al. Melatonin in edible plants identified by radioimmunoassay and by high performance liquid chromatography-mass spectrometry. Journal of Pineal Research, 1995, 18: 28-31
[8] 余雪娜, 李婧娴, 谢永东, 等. 外源褪黑素对高温胁迫下茄子幼苗光合及抗氧化特性的影响. 湖南农业大学学报: 自然科学版, 2016, 42(5): 496-499 [Yu X-N, Li J-X, Xie Y-D, et al. Effects of exogenous melatonin on photosynthetic and antioxidant activity of eggplant seedlings under high temperature stress. Journal of Hunan Agricultural University: Natural Science, 2016, 42(5): 496-499]
[9] Arnao MB, Hernandez-Ruiz J. Growth conditions influen-ce the melatonin content of tomato plants. Food Che-mistry, 2013, 138: 1212-1214
[10] 王蕊, 杨小龙, 须晖, 等. 高等植物褪黑素的合成和代谢研究进展. 植物生理学报, 2016, 52(5): 615-627 [Wang R, Yang X-L, Xu H, et al. Research progress of melatonin biosynthesis and metabolism in higher plants. Plant Physiology Journal, 2016, 52(5): 615-627]
[11] 卞凤娥, 孙永江, 牛彦杰, 等. 高温胁迫下根施褪黑素对葡萄叶片叶绿素荧光特性的影响. 植物生理学报, 2017, 53(2): 257-263 [Bian F-E, Sun Y-J, Niu Y-J, et al. Effect of root-applied melatonin on photosystem Ⅱ in grape leaves under heat stress. Plant Physio-logy Journal, 2017, 53(2): 257-263]
[12] 徐向东, 孙艳, 郭晓芹, 等. 高温胁迫下外源褪黑素对黄瓜幼苗光合作用及叶绿素荧光的影响. 核农学报, 2011, 25(1): 179-184 [Xu X-D, Sun Y, Guo X-Q, et al. Effect of exogenous melatonin on photosynthesis and chlorophyll fluorescence parameters in leaves of cucumber seedlings under high temperature stress. Journal of Nuclear Agricultural Sciences, 2011, 25(1): 179-184]
[13] 陈倩云, 刘海河, 张彦萍, 等. 外源褪黑素对高温胁迫下厚皮甜瓜幼苗光合及抗氧化特性的影响. 河北农业大学学报, 2019, 42(1): 33-37 [Chen Q-Y, Liu H-H, Zhang Y-P, et al. Effects of exogenous melaton in photosynthesis and antioxidant activities of muskmelon seedlings under high temperature stress. Journal of Hebei Agricultural University, 2019, 42(1): 33-37]
[14] Strasser R, Tsimilli MM, Qiang S, et al. Simultaneous in vivo recording of prompt and delayed fluorescence and 820-nm reflection changes during drying and after rehydration of the resurrection plant Haberlea rhodopensis. Biochimica et Biophysica Acta-Biomembranes, 2010, 1797: 1313-1326
[15] Wang K, Zhong M, Wu YH, et al. Overexpression of a chrysanthemum transcription factor gene DgNAC1 improves the salinity tolerance in chrysanthemum. Plant Cell Reports, 2017, 36: 571-581
[16] 赵世杰, 刘华山, 董新纯, 等. 植物生理学实验指导. 北京: 中国农业科技出版社, 1998 [Zhao S-J, Liu H-S, Dong X-C, et al. Guidance of Plant Physiology Experiment. Beijing: China Agricultural Science and Technology Press, 1998]
[17] 左佳琦, 谢佳恒, 薛宇轩, 等. 褪黑素对缓解植物逆境胁迫作用的研究进展. 基因组学与应用生物学, 2014, 33(3): 709-715 [Zuo J-Q, Xie J-H, Xue Y-X, et al. Progress research of melatonin in plant: A multifunctional response to various stress. Genomics and Applied Biology, 2014, 33(3): 709-715]
[18] 李秀, 巩彪, 徐坤. 外源亚精胺对高温胁迫下生姜叶片内源激素及叶绿体超微结构的影响. 中国农业科学, 2015, 48(1): 120-129 [Li X, Gong B, Xu K. Effect of exogenous spermidine on levels of endogenous hormones and chloroplast ultrastructure of ginger leaves under heat stress. Scientia Agricultura Sinica, 2015, 48(1): 120-129]
[19] 马英姿, 张慧, 王志毅, 等. 高温胁迫对蛇足石杉质膜透性及叶绿体结构的影响. 中草药, 2013, 44(18): 2605-2610 [Ma Y-Z, Zhang H, Wang Z-Y, et al. Effects of high temperature stress on plasma membrane permeability and chloroplast structure of Huperzia serrata. Chinese Traditionaland Herbal Drugs, 2013, 44(18): 2605-2610]
[20] 周珩. 外源亚精胺缓解高温胁迫下黄瓜幼苗叶绿素代谢的作用机理研究. 硕士论文. 南京: 南京农业大学, 2016 [Zhou H. Effects of Exogenous Spd on Chlorophyll Metabolism in Cucumber Seedlings under High Temperature Stress. Master Thesis. Nanjing: Nanjing Agricultural University, 2016]
[21] Zhang MP, Zhang CJ, Yu GH, et al. Changes in chloroplast ultrastructure, fatty acid components of thylakoid membrane and chlorophyll a fluorescence transient in flag leaves of a super-high-yield hybrid rice and its parents during the reproductive stage. Journal of Plant Physiology, 2010, 167: 277-285
[22] 李利红, 马培芳, 杨亚军, 等. 外源Ca²⁺对高温强光胁迫下小麦叶绿体D₁蛋白磷酸化及光系统Ⅱ功能的影响. 应用生态学报, 2010, 21(3): 683-688 [Li L-H, Ma P-F, Yang Y-J, et al. Effects of exogenous Ca²⁺ on D₁ protein phosphorylation and PSⅡ performances of wheat leaf chloroplasts under high temperature and illumination stress. Chinese Journal of Applied Ecology, 2010, 21(3): 683-688]
[23] 周君, 肖伟, 陈修德, 等. 喷施氨基酸钙对黄金梨光合性能、果实钙含量及品质的影响. 山东农业大学学报: 自然科学版, 2018, 49(4): 551-555 [Zhou J, Xiao W, Chen X-D, et al. Effect of spraying amino acid-Ca on photosynthetic characteristics, calcium content and quality of ‘Whangkeumbae' pear. Journal of Shandong Agricultural University: Natural Science, 2018, 49(4): 551-555]
[24] 王阳. 外源亚精胺对盐胁迫下薄壳山核桃和山核桃嫁接苗生理生化特性的影响. 硕士论文. 杭州: 浙江农林大学, 2018 [Wang Y. Effects of Exogenous Spermidine on the Physiological and Biochemical Characte-ristics of Grafted Plantlets of Pecan and Hickory under Salt Stress. Master Thesis. Hangzhou: Zhejiang Agriculture and Forestry University, 2018]
[25] 李娟, 彭镇华, 高健, 等. 干旱胁迫下黄条金刚竹的光合和叶绿素荧光特性. 应用生态学报, 2011, 22(6): 1395-1402 [Li J, Peng Z-H, Gao J, et al. Photosynthetic parameters and chlorophyll fluorescence cha-racteristics of Pleioblastus kongosanensis f. aureostriaus under drought stress. Chinese Journal of Applied Eco-logy, 2011, 22(6): 1395-1402]
[26] 吴思政, 梁文斌, 聂东伶, 等. 高温胁迫对不同蓝莓品种光合作用的影响. 中南林业科技大学学报, 2017, 37(11): 1-8 [Wu S-Z, Liang W-B, Nie D-L, et al. Effects of high temperature stress on photosynthesis of different blueberry varieties. Journal of Central South University of Forestry & Technology, 2017, 37(11): 1-8]
[27] 吴雪霞, 张圣美, 张爱冬, 等. 外源褪黑素对高温胁迫下茄子幼苗光合和生理特性的影响. 植物生理学报, 2019, 55(1): 49-60 [Wu X-X, Zhang S-M, Zhang A-D, et al. Effects of exogenous melatonin on photosynthetic and physiological characteristics of eggplant seedlings under high temperature stress. Plant Physiology Journal, 2019, 55(1): 49-60]
[28] 曾庆栋, 许忠民, 张恩慧, 等. 外源褪黑素对高温胁迫下甘蓝幼苗生理特性的影响. 北方园艺, 2017(20): 12-17 [Zeng Q-D, Xu Z-M, Zhang E-H, et al. Effects of exogenous melatonin on physiological characteristics of cabbage seedlings under high temperature stress. Northern Horticulture, 2017(20): 12-17]
[29] 付佳佳, 韩玉林, 李亚亚, 等. Pb、Cd单一及复合胁迫对花菖蒲幼苗生长及部分生理指标的影响. 植物资源与环境学报, 2010, 19(3): 23-27 [Fu J-J, Han Y-L, Li Y-Y, et al. Effects of single and combined stresses of Pb, Cd on growth and some physiological indexes of Iris ensata var. hortensis seedling. Journal of Plant Resources and Environment, 2010, 19(3): 23-27]
[30] Sang QQ, Shu S, Shan X, et al. Effects of exogenous spermidine on antioxidant system of tomato seedlings exposed to high temperature stress. Russian Journal of Plant Physiology, 2016, 63: 645-655
[31] 于金平, 俞珊, 梁有旺, 等. NaCl胁迫对美国白蜡幼苗部分生理指标的影响. 植物资源与环境学报, 2014, 23(1): 110-112 [Yu J-P, Yu S, Liang Y-W, et al. Effect of NaCl stress on some physiological indexes of Fraxinus americana seedling. Journal of Plant Resources and Environment, 2014, 23(1): 110-112]
[32] 宋文静, 赵永长, 况帅, 等. 干旱胁迫下黄腐酸钾对烤烟幼苗保水渗透调节能力的影响. 中国烟草科学, 2017, 38(6): 61-66 [Song W-J, Zhao Y-C, Kuang S, et al. Effect of fulvic acid potassium on osmotic adjustment ability of flue-cured tobacco seedlings under drought stress. Chinese Tobacco Science, 2017, 38(6): 61-66]
[33] 吴燕, 连洪燕, 牟雪姣, 等. 干旱胁迫下叶面喷施褪黑素对滁菊幼苗生理生化特性的影响. 西北植物学报, 2016, 36(11): 2241-2246 [Wu Y, Lian H-Y, Mou X-J, et al. Effects of foliar spraying exogenous melatonin on physiological and biochemical characteristics of Dendranthema morifolium ‘Chuju' seedlings under drought stress. Acta Botanica Boreali-Occidentalia Sinica, 2016, 36(11): 2241-2246]
[34] 王毅, 陈蕤坤, 朱勋路, 等. 不同热激处理对菊花抗氧化相关酶活性的影响. 北方园艺, 2010(9): 98-101 [Wang Y, Chen R-K, Zhu X-L, et al. Effects of different heat treatment on activity of antioxidant-enzyme of chrysanthemum. Northern Horticulture, 2010(9): 98-101]
[35] 任彩虹, 闫桂琴, 郜刚, 等. 高温胁迫对马铃薯幼苗叶片生理效应的影响. 中国马铃薯, 2007(1): 5-10 [Ren C-H, Yan G-Q, Gao G, et al. Physiological effects of high temperature stress on leaves of potato seedlings. Chinese Potato Journal, 2007(1): 5-10]
[36] 樊婕, 张雪莹, 孙宪芝, 等. 茉莉酸甲酯对菊花抗蚜性的影响. 应用生态学报, 2020, 31(12): 4197-4205 [Fan J, Zhang X-Y, Sun X-Z, et al. Effect of methyl jasmonate on aphid resistance of chrysanthemum. Chinese Journal of Applied Ecology, 2020, 31(12): 4197-4205]

外源褪黑素对高温胁迫下菊花光合和生理特性的影响

Effects of exogenous melatonin on photosynthesis and physiological characteristics of chry-santhemum seedlings under high temperature stress

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	邢红爽, 冯秋红, 史作民, 刘顺, 许格希, 陈健, 巩闪闪. 青藏高原东缘川滇高山栎和西南花楸叶片性状对海拔的响应 [J]. 应用生态学报, 2024, 35(3): 606-614.
[2]	郭葳, 王楠, 张凯悦, 宋沛沛, 马占强. Cu₂O纳米颗粒对小麦幼苗快速叶绿素荧光诱导动力学特征及相关基因的影响 [J]. 应用生态学报, 2024, 35(3): 721-730.
[3]	周丹, 李海燕, 王秀军, 李庆卫. 外源褪黑素对盐胁迫下银杏幼苗渗透调节和抗氧化能力的影响 [J]. 应用生态学报, 2024, 35(2): 431-438.
[4]	甘菲菲, 招礼军, 霍灿灿, 王晟. 光照和水分对四季米仔兰幼苗生长的影响 [J]. 应用生态学报, 2024, 35(2): 439-446.
[5]	张雅婷, 叶旺敏, 熊德成, 吴晨, 黄锦学, 陈仕东, 杨智杰. 杉木幼树光合特性与生长的季节变化及其对土壤增温的响应 [J]. 应用生态学报, 2024, 35(1): 195-202.
[6]	苑淑媛, 张鹏, 沈海龙. 天然更新红松苗针叶光合和解剖特性对不同郁闭环境的响应 [J]. 应用生态学报, 2023, 34(9): 2314-2320.
[7]	冷寒冰, 万宁海, 刘群录. 香樟冠层光环境对叶片功能性状和光合特性的影响 [J]. 应用生态学报, 2023, 34(8): 2113-2122.
[8]	王复标, 杨小龙, 康华靖, 叶子飘. 不同环境条件下光合作用对光响应模型的研究进展 [J]. 应用生态学报, 2023, 34(7): 1995-2005.
[9]	上官虹玉, 王传宽, 全先奎. 兴安落叶松叶化学计量特征与光合性状的权衡及其种源差异 [J]. 应用生态学报, 2023, 34(6): 1483-1490.
[10]	张鑫, 马迎杰, 齐边斌, 于波, 吕德国, 秦嗣军. 独脚金内酯类似物GR24对山定子幼苗低氮胁迫的缓解作用 [J]. 应用生态学报, 2023, 34(6): 1592-1600.
[11]	张慧玉, 岳丹斐, 潘晓娇, 郝露露, 刘伟成, 郑春芳. 5-羟色胺在红树植物秋茄幼苗抗寒中的作用 [J]. 应用生态学报, 2023, 34(5): 1263-1271.
[12]	张鹏飞, 刘朋召, 王成龙, 邓明珠, 林延荣, 任小龙, 陈小莉. 大气NH₃浓度升高对不同施氮水平下小麦叶片光合特征和籽粒产量的影响 [J]. 应用生态学报, 2023, 34(4): 1009-1014.
[13]	张鸿志, 张文娥, 樊卫国, 欧阳章维, 蔡虎, 郝振坤, 王瑞璞, 潘学军. 核桃/刺梨复合种植对刺梨生长、果实产量、品质和土壤性质的影响 [J]. 应用生态学报, 2023, 34(3): 699-707.
[14]	惠凯善, 吴召汉, 张永丽. 节水补灌下施磷量对小麦不同茎蘖光合和衰老特性及产量的影响 [J]. 应用生态学报, 2023, 34(2): 451-462.
[15]	王晶, 伏兵哲, 李淑霞, 王星, 宋文学, 叶雨浓, 胡鹏飞, 王通锐. 外源褪黑素对干旱胁迫下沙芦草幼苗生长和生理特性的影响 [J]. 应用生态学报, 2023, 34(11): 2947-2957.