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应用生态学报 ›› 2009, Vol. 20 ›› Issue (10): 2406-2410.

• 研究报告 • 上一篇    下一篇

渗透胁迫下不同抗旱性小麦幼苗氨同化差异

张慧娜;王志强;崔国金;林同保**   

  1. 河南农业大学农学院作物生长发育调控省部共建教育部重点实验室, 郑州 450002
  • 出版日期:2009-10-19 发布日期:2009-10-19

Difference in seedlings ammonium assimilation of wheat cultivars with different drought resistance under osmotic stress

ZHANG Hui-na|WANG Zhi-qiang|CUI Guo-jin|LIN Tong-bao   

  1. Ministry of Education Key Laboratory for Crop Growth and Development Regulation, College of Agronomy, Henan Agricultural University, Zhengzhou 450002, China
  • Online:2009-10-19 Published:2009-10-19

摘要: 在渗透胁迫下,测定了不同抗旱性小麦(抗旱性强的品种洛旱6号和抗旱性弱的品种周麦18)幼苗氨同化酶及相关参数的变化.结果表明:小麦生物量在渗透胁迫下明显降低,且抗旱性弱的周麦18降幅较大.铵态氮含量随胁迫程度的增加而增加,且周麦18增加较明显;谷氨酰胺合成酶(GS)活性在不同抗旱性品种间表现不同,抗旱性强的洛旱6号在低渗透胁迫下显著增加,在高渗透胁迫下明显降低,而周麦18随胁迫程度的增加逐渐降低;依赖还原型辅酶Ⅰ的谷氨酸脱氢酶(NADH-GDH)活性随胁迫程度的增加逐渐加大,低渗透胁迫下周麦18增加较明显,高渗透胁迫下洛旱6号增幅较大;依赖氧化型辅酶Ⅰ的谷氨酸脱氢酶(NAD+-GDH)和依赖氧化型辅酶Ⅱ的异柠檬酸脱氢酶(NADP-ICDH)活性均随胁迫程度的增加而增加,周麦18的NAD+-GDH活性、洛旱6号的NADP-ICDH活性增幅较大.表明小麦抗旱性的提高与铵态氮同化的增强有关,低渗透和高渗透胁迫下分别依赖GS和NADH-GDH活性的增加.

关键词: 小麦, 抗旱性, 渗透胁迫, 氨同化, 土壤有机碳, 小波变换, 尺度相关, 偏相关系数, 广东山区

Abstract: Taking wheat cultivars drought-resistant Luohan-6 and drought-sensitive Zhoumai-18 as test objects, their seedlings ammonium assimilation enzyme activities and related parameters were determined under osmotic stress. The plant biomass had an obvious decrease under osmotic stress, with a larger decrement for Zhoumai-18 than Luohan-6. Osmotic stress increased the plant ammonium content, especially for Zhoumai-18. The glutamine synthetase (GS) activity varied with wheat cultivars. For Luohan-6, the GS activity increased significantly under low osmotic stress but decreased under high osmotic stress; while for Zhoumai-18, the GS activity decreased with increasing osmotic stress. The NADH-dependent glut
amate dehydrogenase (NADH-GDH) increased with increasing osmotic stress, with a marked increment under low osmotic stress for Zhoumai-18, and under high osmotic stress for Luohan-6. The NAD+-dependent glutamate dehydrogenase (NAD+-GDH) and NADP-dependent isocitrate dehydrogenase (NADP-ICDH) activities also increased with increasing osmotic stress, with a greater increment of NAD+-GDH activity for Zhoumai-18, and of NADP-ICDH activity for Luohan-6. It was suggested that the increased drought resistance of wheat plants could be related to the incre
ased ammonium assimilation resulted from the enhanced GS and NADH-GDH activities under low and high osmotic stress, respectively.

Key words: wheat, drought resistance, osmotic stress, ammonium assimilation, soil organic carbon, wavelet transform, multi-scale correlation, partial correlation coefficient,  mountainous area of Guangdong Province.