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应用生态学报 ›› 1997, Vol. 8 ›› Issue (5): 527-530.

• 研究论文 • 上一篇    下一篇

粉刺侧孢霉产木质素过氧化物酶酶活性与pH值的关系

丁佐龙, 高慧   

  1. 安徽农业大学林产工业研究所,合肥 230036
  • 收稿日期:1997-07-21 修回日期:1997-08-28 出版日期:1997-09-25 发布日期:1997-09-25
  • 基金资助:

    中英友好奖学金资助项目

Relationship between lignin peroxidase activity of Phanerochaete chrysosporium and pH value

Ding Zuolong, Gao Hui   

  1. Anhui Agricultural University, Hefei 230036
  • Received:1997-07-21 Revised:1997-08-28 Online:1997-09-25 Published:1997-09-25

摘要: 研究了粉刺侧孢霉(Phanerochaetechrysosporium)在以微晶纤维素为C源时,木素过氧化酶与pH值的关系.试验采用37~39°C下液体振荡培养,藜芦醇为产酶诱导剂,测试不同起始pH值所得木素过氧化物酶活性.结果表明,起始pH值6.0所得酶活最高达0.126U·ml-1,说明以微晶纤维素代替葡萄糖为C源,其最佳pH值相对较高.菌丝球直径与酶活呈正相关.

关键词: 粉刺侧孢霉, 微晶纤维素, 木质素过氧化物酶, pH值, 微生物生物量碳, 微生物生物量氮, 氮沉降, 天然常绿阔叶林

Abstract: Phanerochatet chrysosporium was liquid cultured with avicel as carbon source and with veratric alcohol as enzyme inducing agent.Determinations of its lignin peroxidase activity at different initial pH values showed that the highest enzyme activity was obtained at pH 6.0, indicating that the optimum pHvalue was relatively higher when avicel was used to substitute glucose as a carbon source.The enzyme activity had a positive relationship with the diameter of hyphal pellet.

Key words: Phanerochaete chrysosporium, Avicel, Lignin peroxidase, pH valuei, natural evergreen broad-leaved forest, microbial biomass nitrogen, nitrogen deposition, microbial biomass carbon