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应用生态学报 ›› 1997, Vol. 8 ›› Issue (5): 531-534.

• 研究论文 • 上一篇    下一篇

主要生态环境变化对离体瘤胃菌丛降解粗纤维能力的影响

胡代泽, 邓茂常, 郎家文, 谭祖群, 王淑娟, 杨忠   

  1. 四川省自然资源研究所,成都 610015
  • 收稿日期:1995-10-20 修回日期:1996-08-09 出版日期:1997-09-25 发布日期:1997-09-25
  • 基金资助:

    国家自然科学基金资助项目(39270017)

Influence of eco-environment on crude fibre resolvability of ruminal microbial flora in vitro culture

Hu Daize, Deng Maochang, Lang Jiawen, Tan Zuqun, Wang Shujuan, Yang Zhong   

  1. Sichuan Natural Resources Institute, Chengdu 610015
  • Received:1995-10-20 Revised:1996-08-09 Online:1997-09-25 Published:1997-09-25

摘要: 瘤胃微生物动物体外发酵是在模拟反刍动物瘤胃生态环境的装置中进行的.用长期在动物体外培养和筛选、继代的瘤胃菌丛905231在人工瘤胃装置中研究主要生态条件变化对其降解粗纤维能力的影响,发现在温度30~40℃,扰动60~72r·min-1、20min·h-1和底物C/N比为10/1的生态条件下分解粗纤维的能力最强.发酵5d可达到纤维分解率36~41%的水平.

关键词: 瘤胃菌丛, 生态条件, 粗纤维分解率, 酶, 微生物, 砂姜黑土, 耕作方式, 氮素, 小麦/玉米复种系统

Abstract: With the equipments simulating the environment in a ruminant's paunch the in vitro fermentation of ruminal microbial flora was performed to study the influence of major ecological conditions on crude fibre resolvability. It shows that the crude fibre resolvability is the strongest at the temperature of 30~40℃, stirring intensity of 60~72 r·min-1 with 20 min·h-1 and C/N of 10/1 in culture medium. The fibre resolving rate may reach 36~41% after five days of fermentation.

Key words: Ruminal microbial flora, Ecological condition, Crude fibre resolvability, lime concretion black soil, microorganism, winter-wheat/summer maize multiple cropping system, enzyme, tillage, nitrogen